Figure S5 from Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells

Abstract

<p>(A) CAR T cells, day 0 before infusion, were characterized by flow cytometry. Data shown is mean +/- s.e.m. of three independent experiments. "ns" (not significant) by unpaired t test. (B) Representative flow cytometry plots of CAR T cell CD8+ and CD4+ ratios day 5 after infusion referenced in Figure 2D. (C) Representative flow cytometry plots of PD-1, LAG-3, and TIM-3 inhibitory receptor expression of CD8+ CAR T cells referenced in Figure 2E. (D) CAR T cells, day 5 after infusion, were characterized by flow cytometry. m1928�IL-12 CAR T cells, gated on CD8+ T cells, expressed higher levels of double positive inhibitory receptors, LAG-3;TIM-3 (*p=0.0397) and LAG-3;PD-1 (*p=0.001), and showed a trend toward higher levels of TIM-3;PD-1 (*p=0.0563). Data obtained is mean +/- s.e.m. of three independent experiments analyzed by unpaired t test.</p>