Figure S3 from Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells

Abstract

<p>(A) CAR T cells were co-cultured with EL4mCD19 tumor cells and then assessed for cytotoxicity 4 hours later, using a luciferase killing assay. There was no difference in tumor lysis between CAR T cells and vex-GFP tagged CAR T cells (two-way ANOVA). Data shown is representative of two independent experiments. (B) Flow cytometry gating strategy to determine CD8+ and CD4+ ratio and PD-1, TIM-3, and LAG-3 inhibitory receptor expression.</p>