Field Evaluation of Benzopyrene Hydroxylase Induction as a Monitor for Marine Petroleum Pollution

Payne, J. F.

doi:10.1126/science.1251203

Cited by 222 publications

(49 citation statements)

References 2 publications

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“…P-450. Payne (1976) found elevated levels of hepatic aryl hydrocarbon hydroxylase (AHH) activity in fish from petroleum-contaminated sites, and suggested that AHH induction could be used as an indicator of petroleum contamination. Field observations by Kurelec et al (1977 confirmed the induction of AHH acitivity in fish from polluted areas.…”

Section: Introductionmentioning

confidence: 99%

Variation in hepatic aryl hydrocarbon hydroxylase activity in flounder,Platichthys flesus: A baseline study

Tarlebø

Solbakken

Palmork

1985

Helgolander Meeresunters

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This investigation is concerned with the natural variations in aryl hydrocarbon hydroxylase (AHH) activity of flounder (P1atichthys flesus L.) throughout the year. A general trend towards higher activity in males was observed. It became significant during gonadal maturation, a period during which the activity in females was inhibited. Addition of a-naphthoflavone inhibited AHH activity except in females with maturing gonads. Enzyme activity measured at the optimal temperature for incubation showed highest activity during spring followed by a significant decrease during summer. Activity increased again during autumn, followed by a second decrease in the winter season. When activity was calculated based on ambient water temperature at time of sampling, smaller fluctuations between different seasons were observed; the only significant variation was high activity in June. The results obtained indicate that AHH activity is affected by both exogenous and endogenous factors, which should be taken into consideration if AHH activity is used as a biological indicator of marine pollution effects.

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Section: Introductionmentioning

confidence: 99%

Variation in hepatic aryl hydrocarbon hydroxylase activity in flounder,Platichthys flesus: A baseline study

Tarlebø

Solbakken

Palmork

1985

Helgolander Meeresunters

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“…Les premières études effectuées in situ datent des années 70 (PAYNE et PENROSE, 1975 ;PAYNE, 1976). L'induction peut être quantifiée en particulier par la mesure de l'activité monooxygénase EROD (EthoxyRésorufine-O-Dééthylase) catalysée spécifiquement par le cytochrome P450 1A.…”

Section: Mesure De L'induction De L'erodunclassified

Marqueurs Biochimiques De Pollution Dans Les Écosystèmes Aquatiques Continentaux. Exemples d'UTILISATION Et Perspectives Pour Le Gestionnaire.

Flammarion

Devaux

Garric

2001

Bull. Fr. Pêche Piscic.

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RÉSUMÉEn complément des analyses chimiques, la mesure de biomarqueurs permet de disposer d'informations sur la nature et le niveau de la contamination chimique mais aussi sur la santé des organismes vivants et des populations des écosystèmes aquatiques. Parmi les biomarqueurs, la mesure de marqueurs biochimiques chez les poissons paraît particulièrement intéressante en termes de sensibilité, spécificité et précocité. En cela, les biomarqueurs viennent en complément des mesures traditionnelles de bioindication sur les populations d'invertébrés in situ. L'induction de l'activité enzymatique Ethoxy Résorufine-O-Dééthylase (EROD) est le signe de l'exposition des poissons aux Hydrocarbures Aromatiques Polycycliques (HAPs), PolyChloroBiphényles coplanaires (PCBs) et dioxines, tandis que l'inhibition de l'activité AcétylCholinEstérase (AChE) traduit l'exposition à certains produits phytosanitaires (organophosphorés et carbamates). D'autre part, la mesure d'adduits à l'ADN et de cassures de brins d'ADN renseigne sur la pression génotoxique à laquelle sont exposés les poissons. La synthèse de vitellogénine chez le poisson mâle est quant à elle le signe de l'exposition des poissons à des polluants qui miment l'activité des oestrogènes, entraînant alors un impact sur la reproduction. Ces exemples de biomarqueurs, en voie de validation complète, pourraient être intégrés utilement dans un réseau pérenne de mesure en routine de la qualité et de la santé des écosystèmes aquatiques. Mots-clés BIOCHEMICAL MARKERS OF ENVIRONMENTAL CONTAMINATION IN CONTINENTAL AQUATIC ECOSYSTEMS. EXAMPLES OF USE AND PROSPECTS FOR MANAGEMENT. ABSTRACTIn addition to conventional chemical analyses, biomarkers are considered as useful tools for monitoring chemical contamination and assessing health of organisms and populations in aquatic ecosystems. Biochemical markers represent sensitive and early warning systems of the exposure of aquatic organisms to pollutants, thus complementing more global studies on field invertebrate populations on a long-term time scale. Among biochemical markers, induction of the enzymatic EthoxyResorufin-O-Deethylase (EROD) activity in fish is specific of exposure to Polycyclic Aromatic Hydrocarbons (PAHs), coplanar PolyChlorinated Biphenyis (PCBs) and dioxins. Moreover, inhibition of AcetylChoIinEsterase (AChE) activity can indicate the response of fish to pesticides like organophosphorous compounds and carbamates. Genotoxicity assessment through the measurement of DNA adducts or DNA strand breaks gives rise to relevant information about genetic damage encountered by exposed species. Furthermore, the measure of vitellogenin synthesis in male fish has been successfully used as a biomarker of estrogenic effect of xenobiotics that leads to adverse effects on reproduction. These biomarkers should be integrated in extensive biomonitoring networks in order to complete their validation.

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“…It has been suggested for several years that the cytochrome P-4S0 monooxygenase system (more specifically, AHH activity) should be a convenient moni tor of environmental quality given its character istic induction response (Payne, 1976). Nevertheless the utility of this catalytic activity applied as a diagnostic tool requires cr i tical evaluation by a definition of baseline condi tions and careful consideration of confounding var iables (Stegeman, 1981).…”

Section: Monooxygenase Activities In Aquatic Speciesmentioning

confidence: 99%

“…Elevated levels of AHH in fish are attributed on the basis of kinetic evidence to environmental induction in several cases (Payne, 1976;Stegeman, 1978;Kuralec et al, 1977;. It has been suggested for several years that the cytochrome P-4S0 monooxygenase system (more specifically, AHH activity) should be a convenient moni tor of environmental quality given its character istic induction response (Payne, 1976).…”

Section: Monooxygenase Activities In Aquatic Speciesmentioning

confidence: 99%

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

Klotz¹

1983

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Field Evaluation of Benzopyrene Hydroxylase Induction as a Monitor for Marine Petroleum Pollution

Abstract: Fish from petroleum-contaminated sites in the marine environment have elevated levels of benzopyrene hydroxylase activity in liver and gill tissue. This sublethal response appears to be a practical biological monitor for marine petroleum pollution.

Cited by 222 publications

References 2 publications

Variation in hepatic aryl hydrocarbon hydroxylase activity in flounder,Platichthys flesus: A baseline study

Variation in hepatic aryl hydrocarbon hydroxylase activity in flounder,Platichthys flesus: A baseline study

Marqueurs Biochimiques De Pollution Dans Les Écosystèmes Aquatiques Continentaux. Exemples d'UTILISATION Et Perspectives Pour Le Gestionnaire.

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

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