Field Evaluation of a Real-Time Fluorescence Loop-Mediated Isothermal Amplification Assay, RealAmp, for the Diagnosis of Malaria in Thailand and India

Patel, Jaymin C.; Lucchi, Naomi W.; Srivastava, Priyanka; Lin, Jessica T.; Sugaram, Rungniran; Aruncharus, Supannee; Bharti, Praveen K.; Shukla, Man Mohan; Congpuong, Kanungnit; Satimai, Wichai; Singh, Neeru; Udhayakumar, Venkatachalam; Meshnick, Steven R.

doi:10.1093/infdis/jiu252

Cited by 70 publications

(74 citation statements)

References 34 publications

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“…Several malaria LAMP assays have been described2367918192021222324. However, to date, the only truly field deployment commercial LAMP kits that we are aware of are the Loopamp MALARIA kit (Eiken Chemical Co)22526 and the Illumi gene Malaria LAMP described here.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

Lucchi

Gaye

Diallo

et al. 2016

Sci Rep

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Isothermal nucleic acid amplification assays such as the loop mediated isothermal amplification (LAMP), are well suited for field use as they do not require thermal cyclers to amplify the DNA. To further facilitate the use of LAMP assays in remote settings, simpler sample preparation methods and lyophilized reagents are required. The performance of a commercial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using two sample preparation workflows (simple filtration prep (SFP)) and gravity-driven filtration prep (GFP)) and pre-dispensed lyophilized reagents. Laboratory and clinical samples were tested in a field laboratory in Senegal and the results independently confirmed in a reference laboratory in the U.S.A. The Illumigene Malaria LAMP assay was easily implemented in the clinical laboratory and gave similar results to a real-time PCR reference test with limits of detection of ≤2.0 parasites/μl depending on the sample preparation method used. This assay reliably detected Plasmodium sp. parasites in a simple low-tech format, providing a much needed alternative to the more complex molecular tests for malaria diagnosis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

Lucchi

Gaye

Diallo

et al. 2016

Sci Rep

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“…Additionally, the high sensitivity and specificity of non- falciparum diagnosis observed in the current study is similar with previous controlled laboratory studies conducted in Calgary and London (31, 35). A major benefit of LAMP over RDT is the identification of non- falciparum cases which is critical for regions approaching elimination in Southeast Asia and Latin America (30). We have demonstrated equal efficacy of LAMP-based pathogen infection detection utilizing the NINA electricity-free platform heaters and a programmable PCR thermo-cycler for consistent maintenance of temperature throughout the assay.…”

Section: Discussionmentioning

confidence: 99%

“…Along with in-house methods, a commercial kit for LAMP assay was developed by Eiken Chemical Company, Japan and evaluated (14, 31). Real-time fluorescence LAMP with a portable, rechargeable fluorescence reader was also used for malaria detection in the field (30). Importantly, the LAMP method is robust and tolerant to amplification in the presence of blood impurities and therefore does not require pure RNA/DNA like PCR.…”

Section: Introductionmentioning

confidence: 99%

NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria

Mohon

Lee

Bayih

et al. 2016

Diagnostic Microbiology and Infectious Disease

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Microscopy and field adaptable rapid diagnostic tests (RDTs) are not sensitive and specific in certain conditions such as poor training of microscopists, lack of electricity, or the inability to detect non-falciparum malaria. More sensitive point of care testing (POCT) would reduce delays in diagnosis and initiation of therapy. In the current study, we have evaluated the efficacy of non-instrumented nucleic acid amplification (NINA) coupled with LAMP for detection of traveler’s malaria (n = 140) in comparison with microscopy, nested PCR, and the only FDA-approved rapid diagnostic test. NINA-LAMP was 100% sensitive and 98.6% specific when compared to nested PCR. For non-falciparum detection, NINA-LAMP sensitivity was 100% sensitive compared to nested PCR whereas RDT sensitivity was 71%. LAMP is highly sensitive and specific for symptomatic malaria diagnosis regardless of species in a POCT setting.

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“…6,7 The technical requirements for LAMP are much less onerous than traditional PCR, 7 and as shown here, LAMP for meningococcal disease is rapid and accurate. The relative simplicity of LAMP makes the test an attractive option.…”

mentioning

confidence: 77%

Illuminating meningococcal diagnosis with LAMP

Bahr

Boulware

2015

The Lancet Infectious Diseases

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Field Evaluation of a Real-Time Fluorescence Loop-Mediated Isothermal Amplification Assay, RealAmp, for the Diagnosis of Malaria in Thailand and India

Abstract: RealAmp was comparable to PCR in detecting malaria parasites and shows promise as a tool to detect submicroscopic infections in malaria control and elimination programs worldwide.

Cited by 70 publications

References 34 publications

Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria

Illuminating meningococcal diagnosis with LAMP

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