Field Applicability of a Rapid‐Format Anti–Ov‐16 Antibody Test for the Assessment of Onchocerciasis Control Measures in Regions of Endemicity

Lipner, Ettie M.; Dembélé, N.; Souleymane, Sanou; Alley, William Soumbey; Prevots, D. Rebecca; Toé, Laurent; Boatin, B A; Weil, Gary J.; Nutman, Thomas B.

doi:10.1086/505081

Cited by 85 publications

(82 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These surveys should include more sensitive diagnostic methods including the detection of O. volvulus DNA in skin snips [32] and specific antibodies using the OV16 immunochromatic cards [23,33] to exclude very low-level infections and histologic analysis of the viability and fertility of female worms in onchocercal nodules (if present). Detailed vectors studies might be required to examine the flight range and dispersal patterns of Simulium blackflies from communities in the Río Cayapas where transmission persists because of the potential risk of dispersal of infected Simulium .…”

Section: Discussionmentioning

confidence: 99%

Impact of long-term treatment of onchocerciasis with ivermectin in Ecuador: potential for elimination of infection

Vieira

Cooper

Lovato

et al. 2007

BMC Med

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of long-term treatment of onchocerciasis with ivermectin in Ecuador: potential for elimination of infection

Vieira

Cooper

Lovato

et al. 2007

BMC Med

View full text Add to dashboard Cite

show abstract

“…Blood samples were drawn by digital puncture with a sterile lancet. In 2001, capillary blood samples (collected by finger prick) were obtained from children and examined using Ov-16 card tests as described by Lipner et al [21]. In 2004 and 2007 capillary blood samples were spotted onto filter paper and processed at the Centers for Disease Control (CDC) in Guatemala, using the ELISA technique for IgG4 antibodies against the Ov-16 recombinant antigen [22] following the methodology described by Linblade et al [23] in 2007.…”

Section: Methodsmentioning

confidence: 99%

Elimination of onchocerciasis from Colombia: first proof of concept of river blindness elimination in the world

et al. 2018

View full text Add to dashboard Cite

BackgroundOnchocerciasis is a chronic parasitic infection originally endemic in 13 discrete regional foci distributed among six countries of Latin America (Brazil, Colombia, Ecuador, Guatemala, Mexico and Venezuela). In Colombia, this disease was discovered in 1965 in the Pacific Coast of the country. The National Onchocerciasis Elimination Program was established in 1993 with the aim of eliminating disease morbidity and infection transmission. In 2013, the World Health Organization (WHO) verified Colombia as free of onchocerciasis, becoming the first country in the world to reach such a goal. This report provides the empirical evidence of the elimination of Onchocerca volvulus transmission by Simulium exiguum (s.l.) after 12 years of 6-monthly mass drug administration of Mectizan® (ivermectin) to all the eligible residents living in this endemic area.MethodsFrom 1996 onwards, a biannual community-based mass ivermectin administration programme was implemented, complemented by health education and community participation. In-depth parasitological, serological and entomological surveys were conducted periodically between 1998 and 2007 to evaluate the impact of ivermectin treatment according to the 2001 WHO guidelines. When the interruption of parasite transmission was demonstrated, the drug distribution ceased and a three-year post-treatment surveillance (PTS) period (2008–2010) was initiated.ResultsAfter 23 rounds of treatment, parasitological and ophthalmological assessments showed absence of microfilariae in skin and anterior chamber of the eyes. Serological tests proved lack of antibodies against O. volvulus in children under 10 years-old. A total of 10,500 S. exiguum flies tested by PCR had no L3 infection (infectivity rate = 0.0095%; 95% CI: 0.0029–0.049) during 2004, indicating interruption of parasite transmission. However, biannual ivermectin treatments continued until 2007 followed by a 3-year PTS period at the end of which 13,481 flies were analyzed and no infective flies were found (infectivity rate = 0%; 95% CI: 0.0–0.014).ConclusionsThese results fulfilled the WHO criteria for onchocerciasis elimination. Consequently, in 2013 Colombia was verified as free of onchocerciasis, demonstrating that elimination of this neglected tropical disease is an achievable goal and paving the way for an elimination agenda to be followed by other endemic countries in Latin America and Africa.

show abstract

“…22 The prevalence of immunoglobulin G4 (IgG4) antibodies to Ov-16, a recombinant antigen of Onchocerca volvulus, was used as a measure of infection/exposure in children. [25][26][27] A sample size of 3,000 children is needed to exclude 0.1% antibody prevalence with 95% confidence.…”

Section: Methodsmentioning

confidence: 99%

“…These results were compared with those results collected in the 2005 and 2011 follow-up surveys that used similar methods, with the exception that the 2011 survey added a fifth community. Another variant in the 2011 follow-up survey was that 87 children less than 10 years of age were examined from Buriri (8), Bubungi (27), Bunabutiti (22), Bunabatsu (15), and Bukikoso (15) communities. Two skin snips were taken from the iliac crest posteriorly for every selected adult.…”

Section: Methodsmentioning

confidence: 99%

Transmission of Onchocerca volvulus by Simulium neavei in Mount Elgon Focus of Eastern Uganda Has Been Interrupted

Katabarwa¹,

Lakwo²,

Habomugisha³

et al. 2014

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The study determined that Simulium neavei-transmitted onchocerciasis in Mount Elgon onchocerciasis focus had been interrupted. Annual mass treatment with ivermectin changed to two times per year along with vector elimination in 2007. Then, baseline microfilaria (mf) prevalence data of 1994 in five sentinel communities were compared with follow-up data in 2005 and 2011. Blood spots from 3,051 children obtained in 2009 were analyzed for Onchocerca volvulus immunoglobulin G4 antibodies. Fresh water crab host captures and blackflies collected indicated their infestation with larval stages of S. neavei and presence or absence of the vector, respectively. Mf rates dropped from 62.2% to 0.5%, and 1 (0.03%) of 3,051 children was positive for O. volvulus antibodies. Crab infestation dropped from 41.9% in 2007 to 0%, and S. neavei biting reduced to zero. Both remained zero for the next 3 years, confirming interruption of onchocerciasis transmission, and interventions were halted.

show abstract

Field Applicability of a Rapid‐Format Anti–Ov‐16 Antibody Test for the Assessment of Onchocerciasis Control Measures in Regions of Endemicity

Abstract: The Ov-16 card test is field applicable, exhibits high sensitivity and specificity for O. volvulus infection, and has great potential as a tool for surveillance and for evaluating the success of onchocerciasis control measures.

Cited by 85 publications

References 16 publications

Impact of long-term treatment of onchocerciasis with ivermectin in Ecuador: potential for elimination of infection

Impact of long-term treatment of onchocerciasis with ivermectin in Ecuador: potential for elimination of infection

Elimination of onchocerciasis from Colombia: first proof of concept of river blindness elimination in the world

Transmission of Onchocerca volvulus by Simulium neavei in Mount Elgon Focus of Eastern Uganda Has Been Interrupted

Contact Info

Product

Resources

About