Rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in South America particularly in Brazil where few epidemiological reports have been published. We describe a serosurvey for Rickettsia rickettsii, R. typhi, Coxiella burnetii, Bartonella henselae, B. quintana, and Ehrlichia chaffeensis in 437 healthy people from a Brazilian rural community. The serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 was used. The seroprevalence rates for R. rickettsii, R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis were respectively 1.6% (7 samples (WHO 1993, Rozental et al. 2002, Walker 2004a. Until recently the rickettsiales order comprised the genus Rickettsia, Coxiella, Bartonella, Orientia, Anaplasma, and Ehrlichia (Raoult & Roux 1997) and despite of the resulting withdraw of some of them from this order especially due to 16S rRNA studies, they all can be functionally considered rickettsial agents (Raoult & Roux 1997, Walker 2004b.Most of the available publications of Brazilian rickettsial infections have focused on spotted fever group rickettsioses especially R. rickettsii infections (Lemos et al. 1994, Del Guercio et al. 1997, Rozental et al. 2002, Galvão et al. 2003. This issue has been extensively reviewed recently by Galvão (2004). Despite of the fact that new rickettsial agents have been described more recently in Brazil (Oliveira et al. 2002), the whole scenario of rickettsial infections still have black holes reinforcing the nonexistence concept for such infections in this part of the world.The Piau county as well as the southern part of the state of Minas Gerais had been considered free of any rickettsial diseases until 1994 when a family cluster of Brazilian spotted fever was reported (Hallack et al. 1996). Thereafter reports of Brazilian spotted fever have been Given these above mentioned facts and the much-appropriated local ecologic conditions for rickettsial infections we choose this particular region for studying the seroprevalence rates for R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis as well as R. rickettsii infections among healthy individuals from a Brazilian rural community.
MATERIALS AND METHODSSerum samples from 437 healthy individuals of both sexes aged from 5 to 92 years old, living in the county of Piau, state of Minas Gerais, Brazil were tested for the presence of R. rickettsii, R. typhi, C. burnetii phase I and phase II, B. henselae, B. quintana, and E. chaffeensis IgG and IgM antibodies. This county (Fig. 1), located at latitude 21º30'34''S and longitude 43º19'25''W, has 3008 inhabitants, 58% of them living in the small village and the remainders scattered throughout the county, mostly in small farms. The local economics is somehow linked to cattle farming and the entire population has continuous contact with several domestic animals. Tick attack history is widespread affecting roughly 100% of the population. From March to April 2001, health officials offe...