Fibrotic skin diseases. Clinical presentations, etiologic considerations, and treatment options

Uitto, J.

doi:10.1001/archderm.126.5.661

Cited by 14 publications

(9 citation statements)

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“…Scleroderma is a rare autoimmune disease that involves fibrosis of the skin and in some cases other internal tissues and organs such as skeletal muscle, lungs, gastrointestinal tract, and kidneys. Skin trauma, surgery wounds, and metabolic and immunologic disorders can also underlie cutaneous scars (Uitto and Jimenez, 1990).…”

Section: Dermal Fibrosismentioning

confidence: 99%

Mesenchymal Stem Cells in Fibrotic Disease

et al. 2017

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Fibrosis is associated with organ failure and high mortality and is commonly characterized by aberrant myofibroblast accumulation. Investigating the cellular origin of myofibroblasts in various diseases is thus a promising strategy for developing targeted anti-fibrotic treatments. Recent studies using genetic lineage tracing technology have implicated diverse organ-resident perivascular mesenchymal stem cell (MSC)-like cells and bone marrow-MSCs in myofibroblast generation during fibrosis development. In this Review, we give an overview of the emerging role of MSCs and MSC-like cells in myofibroblast-mediated fibrotic disease in the kidney, lung, heart, liver, skin, and bone marrow.

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Section: Dermal Fibrosismentioning

confidence: 99%

Mesenchymal Stem Cells in Fibrotic Disease

et al. 2017

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“…Fibroblast cells have been cultured in vitro from several species and tissues (Normand and Karasek 1995;Harper et al 2007;Li et al 2009a, b). These cultured cells have been used for in vitro analysis of fibroblast growth, migration, and collagen metabolism (Gay et al 1976), to study glycoprotein metabolism in normal and mannasidasedeficient goats (Hancock et al 1986;Amancha et al 2007), to study the pathophysiological processes (Roperto et al 1999), and to diagnose inborn errors of metabolism and pathophysiology of fibrotic skin diseases in humans (Uitto and Jimenez 1990). Fibroblasts play a crucial role in wound healing and thus have been extensively studied to develop biomaterial implants, secreting growth factors for treatment of diabetic foot ulcers (Mansbridge et al 1999;Han and Kim 2009).…”

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confidence: 99%

Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines

Singh

Sharma

2010

In Vitro Cell.Dev.Biol.-Animal

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Three different commercially available media, known to support human and porcine-specific fibroblast cultures, were tested for their growth potential on goat skin explants. Although outgrowth of fibroblasts was observed in all media tested, irrespective of breed, porcine-specific media exhibited higher rate of growth. Using this media, three fibroblast cell lines (GSF289, GSF737, and GSF2010) from ear skin explants of normal healthy dairy goats of Kiko and Saanen breed were successfully established in culture. Liquid nitrogen stocks of these frozen cells had a viability rate of 96.2% in in vitro cultures. These cells were morphologically indistinguishable from the cell stocks prior to freezing. Analysis of the growth of a fifth passage culture revealed an 'S' shaped growth curve with a population doubling time of 25 h. The cell lines were found negative for microbial, fungal, and mycoplasma contaminations. These goat skin fibroblast lines and the simple method of their isolation and freezing with high rate of viability will provide additional tools to study molecular mechanisms that regulate fibroblast function and for genetic manipulation of small ruminants.

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“…Excessive production and cross-linking of collagen fibers result in abnormal (hypertrophic and keloid) scar tissue formation that leads to tissue stiffening. 67,68 Because dermal fibroblasts play a critical role in abnormal scar tissue formation, we show the capability to culture human dermal fibroblasts in EKGel and characterize their metabolic activity as well as their collagen and fibronectin synthesis.…”

Section: ■ Introductionmentioning

confidence: 99%

Nanofibrillar Hydrogel Recapitulates Changes Occurring in the Fibrotic Extracellular Matrix

et al. 2021

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Fibrosis is a pathological condition that leads to excessive deposition of collagen and increased tissue stiffness. Understanding the mechanobiology of fibrotic tissue necessitates the development of effective in vitro models that recapitulate its properties and structure; however, hydrogels that are currently used for this purpose fail to mimic the filamentous structure and mechanical properties of the fibrotic extracellular matrix (ECM). Here, we report a nanofibrillar hydrogel composed of cellulose nanocrystals and gelatin, which addresses this challenge. By altering the composition of the hydrogel, we mimicked the changes in structure, mechanical properties, and chemistry of fibrotic ECM. Furthermore, we decoupled the variations in hydrogel structure, properties, and ligand concentration. We demonstrate that this biocompatible hydrogel supports the three-dimensional culture of cells relevant to fibrotic diseases. This versatile hydrogel can be used for in vitro studies of fibrosis of different tissues, thus enabling the development of novel treatments for fibrotic diseases.

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Fibrotic skin diseases. Clinical presentations, etiologic considerations, and treatment options

Cited by 14 publications

References 0 publications

Mesenchymal Stem Cells in Fibrotic Disease

Mesenchymal Stem Cells in Fibrotic Disease

Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines

Nanofibrillar Hydrogel Recapitulates Changes Occurring in the Fibrotic Extracellular Matrix

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