Fibrocytes and fibroblasts—Where are we now

Chong, Sy Giin; Sato, Seidai; Kolb, Martin; Gauldie, Jack

doi:10.1016/j.biocel.2019.105595

Cited by 55 publications

(60 citation statements)

References 44 publications

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“…In previous studies, fibrocytes have been identified as spindle‐shaped cells that meet the minimum definition criteria, including the detection of collagen production and the surface expression of CD45 and/or CD34 5,9 . In spite of insufficiency, these criteria still apply to this day, as there are still no specific markers for fibrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Fibrocytes can migrate into damaged tissues and usually contribute to their repair 6–8 . The key features of fibrocytes include the expression of myeloid markers such as CD34, CD45, CD14 and CD11a, CD11b, the production of extracellular matrix proteins such as collagen‐I, vimentin, and α‐smooth muscle actin, and the secretion of various humoral factors that activate resident fibroblasts; they also have the potential to differentiate into fibroblasts 5,9 …”

Section: Introductionmentioning

confidence: 99%

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Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain

Kawano

Koyama

Nishimura

et al. 2020

Immunity Inflam & Disease

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Introduction Fibrocytes are emerging myeloid‐derived circulating cells that can migrate into damaged tissues and usually contribute to their repair. Key features of fibrocytes include the expression myeloid markers, production of extracellular matrix proteins, and secretion of various humoral factors that activate resident fibroblasts; they also have the potential to differentiate into fibroblasts. However, no specific surface markers have been identified to identify fibrocytes in vivo. One reason could be that the method used to detect fibrocytes requires intracellular collagen staining. Methods In the present study, to establish an improved method for the detection of lung fibrocytes and to analyze viable fibrocytes, we used collagen I(α)2‐green fluorescent protein (Col‐GFP) reporter mice, which had undergone the intratracheal instillation of bleomycin (BLM). Results Using flow cytometry to gate out cells with autofluorescence, we clearly found that CD45+ GFP+ cells resided in the lungs of Col‐GFP mice at a steady state and these cells increased after BLM injury, peaking at Day 14. These cells expressed not only known cell surface markers of fibrocytes, but also some novel markers, in addition to a low level of collagen I in comparison to CD45− GFP+ cells. Conclusion Our findings suggest that the improved method can be a useful for the detection of pure lung fibrocytes and allows us to further analyze the characteristics of viable fibrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain

Kawano

Koyama

Nishimura

et al. 2020

Immunity Inflam & Disease

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“…Furthermore, chronic periaortitis patients showed circulating FC elevation when compared to healthy donors, further suggesting predictive biomarker value in fibrotic diseases. FC may well be associated with cancer [189]. However, the field of cancer-associated FC or tissue FC is not well understood [190] and consequently remains to be researched.…”

Section: Circulating Fibroblast-like Cells Clinical Usefulnessmentioning

confidence: 99%

The Blood Circulating Rare Cell Population. What Is It and What Is It Good for?

Schreier

Triampo

2020

Cells

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Blood contains a diverse cell population of low concentration hematopoietic as well as non-hematopoietic cells. The majority of such rare cells may be bone marrow-derived progenitor and stem cells. This paucity of circulating rare cells, in particular in the peripheral circulation, has led many to believe that bone marrow as well as other organ-related cell egress into the circulation is a response to pathological conditions. Little is known about this, though an increasing body of literature can be found suggesting commonness of certain rare cell types in the peripheral blood under physiological conditions. Thus, the isolation and detection of circulating rare cells appears to be merely a technological problem. Knowledge about rare cell types that may circulate the blood stream will help to advance the field of cell-based liquid biopsy by supporting inter-platform comparability, making use of biological correct cutoffs and “mining” new biomarkers and combinations thereof in clinical diagnosis and therapy. Therefore, this review intends to lay ground for a comprehensive analysis of the peripheral blood rare cell population given the necessity to target a broader range of cell types for improved biomarker performance in cell-based liquid biopsy.

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“…Moreover, MSC-derived CAFs in breast cancer tissues exhibited reduced PDGF-Rα expression and augmented angiogenic activities, compared with resident fibroblast-derived CAFs [ 28 ]. Another type of bone marrow-derived cells, fibrocytes, which express type I collagen protein as well as a hematopoietic marker, CD45 [ 29 ], exhibited CAF-like phenotypes in chemically-induced rat breast carcinogenesis model [ 30 ]. However, there remain controversies on the contribution of non-resident fibroblasts to the generation of CAFs in solid tumor tissues [ 18 ], due to the lack of specific markers to identify CAFs ( Figure 1 ).…”

Section: Phenotypes and Origins Of Fibroblasts And Cafsmentioning

confidence: 99%

Emergence of Cancer-Associated Fibroblasts as an Indispensable Cellular Player in Bone Metastasis Process

Mukaida

Zhang

Sasaki

2020

Cancers

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Bone metastasis is frequently complicated in patients with advanced solid cancers such as breast, prostate and lung cancers, and impairs patients’ quality of life and prognosis. At the first step of bone metastasis, cancer cells adhere to the endothelium in bone marrow and survive in a dormant state by utilizing hematopoietic niches present therein. Once a dormant stage is disturbed, cancer cells grow through the interaction with various bone marrow resident cells, particularly osteoclasts and osteoblasts. Consequently, osteoclast activation is a hallmark of bone metastasis. As a consequence, the drugs targeting osteoclast activation are frequently used to treat bone metastasis but are not effective to inhibit cancer cell growth in bone marrow. Thus, additional types of resident cells are presumed to contribute to cancer cell growth in bone metastasis sites. Cancer-associated fibroblasts (CAFs) are fibroblasts that accumulate in cancer tissues and can have diverse roles in cancer progression and metastasis. Given the presence of CAFs in bone metastasis sites, CAFs are emerging as an important cellular player in bone metastasis. Hence, in this review, we will discuss the potential roles of CAFs in tumor progression, particularly bone metastasis.

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Fibrocytes and fibroblasts—Where are we now

Cited by 55 publications

References 44 publications

Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain

Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain

The Blood Circulating Rare Cell Population. What Is It and What Is It Good for?

Emergence of Cancer-Associated Fibroblasts as an Indispensable Cellular Player in Bone Metastasis Process

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