Fibrinolysis

Bachman, Frank P.

doi:10.1007/978-1-4613-0753-2_18

Cited by 1 publication

(2 citation statements)

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“…In the process of fibrinolysis, as well as in coagulation, anticoagulation, and the complement system, serine proteases play crucial roles (Bachman, ). They work in amplification cascades where an active serine protease activates another proproteinase (Davie et al, ).…”

Section: Commentarymentioning

confidence: 99%

“…Plasmin is a key serine protease in the fibrinolytic system. Its activation from the zymogen plasminogen results in the dissolution of blood clots, and also promotes pericellular proteolysis, cell migration, and tissue remodeling (Bachman, ; Chapman, ). Interestingly, pathogenic cells recruit plasmin proteolytic activity to their cell surface to facilitate cell invasion and migration through tissue layers (Murakami et al, ).…”

Section: Commentarymentioning

confidence: 99%

See 1 more Smart Citation

Over‐Expression and Purification of Active Serine Proteases and Their Variants from Escherichia coli Inclusion Bodies

Parry

2002

CP Protein Science

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This unit describes the over-expression and purification of active serine proteases and their variants from E. coli inclusion bodies. The strategy includes the folding and purification of a stable zymogen precursor protein, and its later activation with the appropriate convertase to the less stable but active protease. A test to follow the presence of activity in the samples, together with an active-site titration protocol to determine the number of active sites per mole of total protein are provided. It should be emphasized that although most of the protocols described are applied to a specific example, they are fairly representative of the methods and approaches generally used for laboratory-scale preparation of other recombinant serine proteases. The critical steps and how this template protocol can be adapted for the purification of other serine proteases are described.

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Section: Commentarymentioning

confidence: 99%

Section: Commentarymentioning

confidence: 99%