Textile-based stretchable electronic devices are one of the best candidates for future wearable applications, as they can simultaneously provide high compliance and wearing comfort to the human body. Stretchable conductive textile is the fundamental building block for constructing high-performance textile-based stretchable electronic devices. Here, we report a simple strategy for the fabrication of stretchable conductive fabric using commercial knitted cloth as a substrate. Briefly, we coated the fibers of the fabric with a thin layer of poly(styrene-block-butadiene-block-styrene) (SBS) by dip-coating. Then, silver nanoparticles (AgNPs) were loaded on the fabric by sequential absorption and in situ reduction. After loading AgNPs, the conductivity of the fabric could be as high as ∼800 S/m, while its maximal strain at break was higher than 540%. Meanwhile, such fabric also possesses excellent permeability, robust endurance to repeated stretching, long-time washing, and mechanical rubbing or tearing. We further approve that the fabric is less cytotoxic to mammalian skin and antibacterial to microbial, making it safe for on-skin applications. With these multifarious advantages, the fabric developed here is promising for on-skin wearable applications. As a proof-of-concept, we demonstrate its use as an electrode for collecting electrocardiograph signals and electrothermal therapy.