Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model

Beason, David P.; Connizzo, Brianne K.; Dourte, LeAnn M.; Mauck, Robert L.; Soslowsky, Louis J.; Steinberg, David R.; Bernstein, Joseph

doi:10.1016/j.jse.2011.10.021

Cited by 77 publications

(77 citation statements)

References 18 publications

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“…We fabricated collagen scaffolds with different concentrations resulting in different pore sizes and porosities and tested these by ectopic implantation with pressure from surrounding tissues. It was demonstrated that low concentration scaffolds could not sustain their structural integrity and easily collapsed, and that the cells could not infiltrate the collapsed region, similar to that reported by another study [7]. On the other hand, the high concentration scaffold was too compact for cells to infiltrate, because pore sizes of several hundred micrometers and porosity of 90% or higher are conducive for cell infiltration [20].…”

Section: Discussionsupporting

confidence: 69%

“…However, their effects on tenogenic differentiation are usually weaker than tendon-derived decellularized matrices [6]. In addition, synthetic scaffolds have the drawback of poor cell adhesion, and can induce chronic inflammation [7,8]. Hence, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for facilitating repair of massive rotator cuff tendon injuries need to be developed.…”

Section: Introductionmentioning

confidence: 99%

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Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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“…Animals did not need to be restrained for this task as they willingly drank fluid from the syringe after their training period. All animals then underwent bilateral supraspinatus detachment and repair surgeries as described previously in detail [3,27]. Buprenorphine was administered subcutaneously to all animals at a dose of 0.05 mg/kg immediately before surgery and then every 8 to 12 hours for the next 2 days.…”

Section: Methodsmentioning

confidence: 99%

“…The humeral diaphysis was potted in polymethylmethacrylate and placed in a base fixture. Specimens were submerged in a 37°C phosphatebuffered saline bath and tensile tested using an Instron 5543 mechanical test frame (Instron Corp, Norwood MA, USA) using a protocol described previously [3,21]. Briefly, tendons were subjected to 10 cycles of preconditioning between 0.1 and 0.5 N to provide consistent strain history between specimens and then allowed to return to equilibrium over 300 seconds.…”

Section: Methodsmentioning

confidence: 99%

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

Connizzo

Yannascoli

Tucker

et al. 2014

Clinical Orthopaedics &Amp; Related Research

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Background Current clinical treatment after tendon repairs often includes prescribing NSAIDs to limit pain and inflammation. The negative influence of NSAIDs on bone repair is well documented, but their effects on tendon healing are less clear. While NSAIDs may be detrimental to early tendon healing, some evidence suggests that they may improve healing if administered later in the repair process.Questions/purposes We asked whether the biomechanical and histologic effects of systemic ibuprofen administration on tendon healing are influenced by either immediate or delayed drug administration. Methods After bilateral supraspinatus detachment and repair surgeries, rats were divided into groups and given ibuprofen orally for either Days 0 to 7 (early) or Days 8 to 14 (delayed) after surgery; a control group did not receive ibuprofen. Healing was evaluated at 1, 2, and 4 weeks postsurgery through biomechanical testing and histologic assessment. Results Biomechanical evaluation resulted in decreased stiffness and modulus at 4 weeks postsurgery for early ibuprofen delivery (mean ± SD [95% CI]: 10.8 ± 6.4 N/mm [6.7-14.8] and 8.9 ± 5.9 MPa [5.4-12.3]) when compared to control repair .2]) (p = 0.003 and 0.013); however, there were no differences between the delayed ibuprofen group (18.1 ± 7.4 N/mm [14.2-22.1] and 11.5 ± 5.6 MPa [8.2-14.9]) and the control group. Histology confirmed mechanical results with reduced fiber reorganization over time in the early ibuprofen group.

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“…The blend scaffold showed the proliferation of cells throughout the scaffold, whereas the scaffold comprising poly(caprolactone) alone had cells only on the periphery of the scaffold. Yet another group used an aligned nanofibrous scaffold, comprising the same blend of polymers, to successfully repair rotator cuff tendon tears in rat models [59]. Similarly, poly(ethylene glycol) was employed as a sacrificial template in combination with glutaraldehyde cross-linked gelatin fibres.…”

Section: Scaffold Design Strategiesmentioning

confidence: 99%

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

Krishnan

Sethuraman

2013

Nanomaterials and Nanotechnology

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Introduction: Successful tissue engineering strategies leading to the regeneration of a tissue depend on many factors, starting from the choice of appropriate scaffold material, tailoring the surface functionalities and topography, providing the correct amount of chemical and mechanical stimuli at the appropriate time points, and ensuring the uniform and precise localization of cells. Further challenges arise when more than one cell type has to be employed for the effective regeneration of an organ. Importance: Though the use of nanomaterials has improved tissue engineering, many pitfalls still exist that present a roadblock in the translation of tissue engineering strategies to clinical practice. Apart from employing different materials with distinct surface functionalities and mechanical properties, various strategies have been employed to manipulate the surface topography and chemistry of scaffolds to create a biomimetic microenvironment for effective tissue regeneration. Conclusion: This review provides information about the factors influencing tissue engineering, namely geometry, chemistry, mechanics and cells, and the emerging concepts that may well represent the future of regenerative medicine. Electrospinning techniques and their variants, self-assembly, cell-printing techniques and cell sheet engineering, have all been elaborated in detail. These novel techniques may serve to overcome the challenges currently faced in tissue engineering.

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Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model

Cited by 77 publications

References 18 publications

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

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