Ferulic Acid Suppresses Amyloid<i>β</i>Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

Nagai, Noriaki; Kotani, Sachiyo; Mano, Yu; Ueno, Akina; Ito, Yoshimasa; Kitaba, Toshio; Takata, Takumi; Fujii, Nobutaka

doi:10.1155/2017/5343010

Cited by 14 publications

(7 citation statements)

References 38 publications

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“…[30][31][32][33][34][35] We have also reported that the stimulation of ROS hydrogen peroxide augmented gene expression of the proteins related to Ab production, resulting in the production of three types of Ab peptides (Ab 1-40 , Ab , and Ab ) in the human lens epithelial SRA01/04 cells. 36 In addition, we used lens epithelium located on the outer surface of the lens in this FIGURE 7. Gene expression of (A) APP, (B) BACE1, (C) PS1, (D) PS2, (E) ADAM10, (F) NEP, and (G) ECE1 in the lens epithelium of cataract patients with or without cortical opacification.…”

Section: Discussionmentioning

confidence: 99%

Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

Nagai

Mano

Otake

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.We investigated the accumulation of amyloid b (Ab 1-40 , Ab 1-42 , Ab ) in the lens epithelium of patients with opacification of five different types (cortical cataract [COR]; nuclear cataract [NUC]; posterior subcapsular cataract [PSC]; retrodots [RD]; and water clefts [WC]).METHODS. Samples were collected from Japanese patients taken during cataract surgery; Ab levels and mRNA expression were determined by ELISA and a real-time RT-PCR method, respectively.RESULTS. Levels of Ab 1-40 and Ab 1-42 in the lens epithelium of patients with COR, NUC, PSC, RD, and WC showed no significant differences in comparison with transparent lens epithelium. Levels of Ab 1-43 in the lens epithelium of patients with PSC and WC were not detected, and NUC and RD were slightly elevated. In contrast to the results in these cataract types, high Ab 1-43 levels were observed in the lens epithelium of patients with COR, and a close relationship was observed between Ab 1-43 levels and the degree of lens opacification (R ¼ 0.8229, n ¼ 6). The levels of Ab 1-43 were also higher in the lens epithelium of patients with mixed-cataract showing cortical opacification, and the Ab 1-43 levels in the lens epithelium of mixed-cataract patients with cortical opacification was significantly higher than in that of mixed-cataract patients without cortical opacification. In addition, the level of an amyloid precursor protein mRNA in the lens epithelium of mixed-cataract patients with cortical opacification was significantly higher than in transparent lens and mixed-cataract patients without cortical opacification. CONCLUSIONS.We found high levels of Ab 1-43 accumulation in the lens epithelium of Japanese patients with cortical opacification.Keywords: amyloid b, cortical cataract, human, lens epithelium, Japanese A ge-related cataracts are the most common cause of vision loss in the elderly, and the principal cause of blindness in the world. Age-related cataracts are classified by the location of the opacity within the lens: cortical cataracts (COR); nuclear cataracts (NUC); and posterior subcapsular cataracts (PSC). In addition, retrodots (RD) and water clefts (WC) have been found to be associated with visual impairment. Cataracts represent a complex disease with numerous genetic and environmental contributing factors, including smoking, ultraviolet irradiation, diabetes, the cumulative effect of X-rays, nutrition, vitamin C deficiency, hypertension, and alterations in both endocrine and enzymatic equilibria.1-8 On the other hand, it has recently been reported that the accumulation of amyloid b (Ab) peptides in the human lens may be related to the onset of lens opacification, 9-11 but this involvement in terms of etiology and Ab levels is not fully understood. 12 The enzymes a-secretase (a disintegrin and metalloproteinase, ADAM10) 13-15 ; b-secretase (b site APP cleaving enzyme, called BACE1) 16 ; and c-secretase (a presenilin complex, PS1 and PS2) 17 are known to be related to Ab production by cleaving an amyloid precursor protein (APP). The...

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Section: Discussionmentioning

confidence: 99%

Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

Nagai

Mano

Otake

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…The human lens epithelial cell line SRA 01/04 (HLE cell) was cultured under humidified air containing 5% CO 2 at 37 • C, following our previous reports [31,35]. Briefly, HLE cells were cultured in Dulbecco's modified Eagle's medium with gentamicin (10 mg/L) and 10% (v/v) heat-inactivated fetal bovine serum.…”

Section: Measurement Of Toxicity Using Cultured Human Lens Cellsmentioning

confidence: 99%

The Intravitreal Injection of Lanosterol Nanoparticles Rescues Lens Structure Collapse at an Early Stage in Shumiya Cataract Rats

Nagai

Fukuoka

Sato

et al. 2020

IJMS

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We designed an intravitreal injection formulation containing lanosterol nanoparticles (LAN-NPs) via the bead mill method and evaluated the therapeutic effect of LAN-NPs on lens structure collapse and opacification using two rat cataract models (SCR-N, rats with slight lens structure collapse; SCR-C, rats with the combination of a remarkable lens structure collapse and opacification). The particle size of lanosterol in the LAN-NPs was around 50–400 nm. A single injection of LAN-NPs (0.5%) supplied lanosterol into the lens for 48 h, and no irritation or muddiness was observed following repeated injections of LAN-NPs for 6 weeks (once every 2 days). Moreover, LAN-NPs repaired the slight collapse of the lens structure in SCR-N. Although the remarkable changes in the lens structure of SCR-C were not repaired by LAN-NP, the onset of opacification was delayed. In addition, the increase of cataract-related factors (Ca2+ contents, nitric oxide levels, lipid peroxidation and calpain activity levels) in the lenses of SCR-C was attenuated by the repeated injection of LAN-NPs. It is possible that a deficiency of lanosterol promotes the production of oxidative stress. In conclusion, it is difficult to improve serious structural collapse with posterior movement of the lens nucleus with a supplement of lanosterol via LAN-NPs. However, the intravitreal injection of LAN-NPs was found to repair the space and structural collapse in the early stages in the lenses.

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“…The mechanism of action of Cur on AD has been reported to involve the inhibition of Aβ aggregation, an increase in BDNF, and a decrease in tau phosphorylation [ 10 , 30 , 31 ]. On the other hand, research has shown that FA inhibits Aβ production via downregulation of APP and β-secretase [ 32 ], has potent antioxidant properties, and protects neurons from Aβ-induced neurotoxicity mainly although we previously confirmed FA also has anti-amyloidogenic effect for Aβ [ 21 ]. We hypothesized that FA and Cur might complement each other based on such reports.…”

Section: Discussionmentioning

confidence: 92%

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

Ohashi

Tsuji

Oguchi

et al. 2022

IJMS

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Alzheimer’s disease (AD) is a neurodegenerative disease that leads to progressive cognitive decline. Several effective natural components have been identified for the treatment of AD. However, it is difficult to obtain conclusive evidence on the safety and effectiveness of natural components, because a variety of factors are associated with the progression of AD pathology. We hypothesized that a therapeutic effect could be achieved by combining multiple ingredients with different efficacies. The purpose of this study was thus to evaluate a combination treatment of curcumin (Cur) and ferulic acid (FA) for amyloid-β (Aβ)-induced neuronal cytotoxicity. The effect of Cur or FA on Aβ aggregation using thioflavin T assay was confirmed to be inhibited in a concentration-dependent manner by Cur single or Cur + FA combination treatment. The effects of Cur + FA on the cytotoxicity of human neuroblastoma (SH-SY5Y) cells induced by Aβ exposure were an increase in cell viability, a decrease in ROS and mitochondrial ROS, and repair of membrane damage. Combination treatment showed an overall higher protective effect than treatment with Cur or FA alone. These results suggest that the combined action mechanisms of Cur and FA may be effective in preventing and suppressing the progression of AD.

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Ferulic Acid Suppresses AmyloidβProduction in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

Cited by 14 publications

References 38 publications

Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

The Intravitreal Injection of Lanosterol Nanoparticles Rescues Lens Structure Collapse at an Early Stage in Shumiya Cataract Rats

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

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Ferulic Acid Suppresses AmyloidβProduction in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

Cited by 14 publications

References 38 publications

Amyloid β1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

Amyloid β1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

The Intravitreal Injection of Lanosterol Nanoparticles Rescues Lens Structure Collapse at an Early Stage in Shumiya Cataract Rats

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

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Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients

Amyloid β_1–43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients