Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin

Abstract

A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.

Levan-Producing Leuconostoc citreum Strain BD1707 and Its Growth in Tomato Juice Supplemented with Sucrose

“…4). No peaks were found in the anomeric region (5.3 to 4.3 ppm), reflecting the absence of anomeric protons (29). These results provided strong evidence that the polysaccharide from BD1707 is a homopolymer of fructose, which is in accordance with the result of monosaccharide analysis.…”

“…These results provided strong evidence that the polysaccharide from BD1707 is a homopolymer of fructose, which is in accordance with the result of monosaccharide analysis. On the whole, the 1 H NMR spectrum of the BD1707 fructan displayed high similarity to that of the levan from Zymomonas mobilis (ZAG-12L) (29).…”

<b>PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography

“…PVA has already been proposed as support for biomolecule immobilization, such as drugs (Constantin et al, 2007), DNA (Liu et al, 2009), horseradish peroxidase for detoxification of wastewater (Caramori, Fernandes, & Carvalho Junior, 2012) and F1 antigen from Yersinia pestis (Barbosa et al, 2000). Angeli et al (2009) used ferromagnetic levan composite for Cramoll 1,4 purification by affinity chromatography using a 0.3 M glucose solution for elution, but the purified protein presented a decrease in hemagglutinating activity when compared to conventional chromatography methods. These data together indicates our support as a promising biotechnological tool for lectin immobilization.…”

Isolamento de lectinas por cromatografia de afinidade

“…Colombia. 3 YOLUKA ONG Fundación de Investigación en Biodiversidad y Conservación. 4 Instituto Federal do Ceará -IFCE.…”

“…Jaguaribe -Ceará, Brasil. 1 jose.torres@unad.edu.co, 2 myriam.ortega@unad.edu.co, 3 jhon.infante@yoluka.org.co, 4 cicero.carla@uol.com.br…”