Fermented milk with probiotic Lactobacillus rhamnosus S1K3 (MTCC5957) protects mice from salmonella by enhancing immune and nonimmune protection mechanisms at intestinal mucosal level

Kemgang, Tanedjeu Sonfack; Kapila, Suman; Shanmugam, Venkatesa Perumal; Reddi, Srinu; Kapila, Rajeev

doi:10.1016/j.jnutbio.2015.11.018

Cited by 34 publications

(21 citation statements)

References 92 publications

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“…The mice were weighed, and the liver and spleen were weighed immediately after euthanasia. Organ index was calculated as [ 12 ]: Liver or spleen index = [weight of liver or spleen (g)/body weight (g)] × 1000.…”

Section: Methodsmentioning

confidence: 99%

“…Studies have shown that some probiotics promote local immunity and IFN- γ production while inhibiting IL-4 secretion associated with allergies [ 11 ]. Lactic acid bacteria induce sIgA secretion, inhibit the invasion of pathogenic bacteria, and protect the host intestinal tract [ 12 ]. Some probiotics, such as Lactobacillus plantarum , are involved in mediating immune function [ 13 ] and can enhance the immunological function of immunosuppressed mice infected with S. aureus .…”

Section: Introductionmentioning

confidence: 99%

“…LAB induce anti-inflammatory factors, such as IL-4 (Th2 cells), while decreasing the levels of enteric or serum regulatory factors in the late stages of infection. This results in a lower number of Salmonella in the feces [ 12 ]. LAB can inhibit the growth of Escherichia coli , Proteus vulgaris , Enterococcus faecalis , Staphylococcus aureus , and other pathogens under suitable pH conditions; this may be due to lactate production [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Mixed Lactobacillus plantarum Strains Inhibit Staphylococcus aureus Induced Inflammation and Ameliorate Intestinal Microflora in Mice

Ren

Gong

Shu

et al. 2017

BioMed Research International

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Objective. Staphylococcus aureus is an important pathogen that causes intestinal infection. We examined the immunomodulatory function of single and mixed Lactobacillus plantarum strains, as well as their impacts on the structure of the microbiome in mice infected with Staphylococcus aureus. The experiment was divided into three groups: protection, treatment, and control. Serum IFNand IL-4 levels, as well as intestinal sIgA levels, were measured during and 1 week after infection with Staphylococcus aureus with and without Lactobacillus plantarum treatment. We used 16s rRNA tagged sequencing to analyze microbiome composition. IFN-/IL-4 ratio decreased significantly from infection to convalescence, especially in the mixed Lactobacillus plantarum group. In the mixed Lactobacillus plantarum group the secretion of sIgA in the intestine of mice (9.4-9.7 ug/mL) was significantly higher than in the single lactic acid bacteria group. The dominant phyla in mice are Firmicutes, Bacteroidetes, and Proteobacteria. Treatment with mixed lactic acid bacteria increased the anti-inflammatory factor and the secretion of sIgA in the intestine of mice infected with Staphylococcus aureus and inhibited inflammation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mixed Lactobacillus plantarum Strains Inhibit Staphylococcus aureus Induced Inflammation and Ameliorate Intestinal Microflora in Mice

Ren

Gong

Shu

et al. 2017

BioMed Research International

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“…Gagnon et al (2013) showed that Salmonella Typhimurium significantly reduced the TEER of HT29-MTX cells at 90 min of invasion, in agreement with results obtained in this study. Kemgang et al (2016) demonstrated the ability of Salmonella to interfere with monolayer integrity while exposure to Lactobacillus rhamnosus S1K3 cultures aided in maintaining barrier function in the presence of Salmonella. The authors also investigated the expression of claudin-1, a tight junction gene, and observed downregulation in claudin-1 when exposed to Salmonella and upregulation when incubated with L. rhamnosus.…”

Section: Discussionmentioning

confidence: 99%

Effect of fermented milk from Lactococcus lactis ssp. cremoris strain JFR1 on Salmonella invasion of intestinal epithelial cells

Zhang

Corredig

Morales-Rayas

et al. 2019

Journal of Dairy Science

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The process of fermentation contributes to the organoleptic properties, preservation, and nutritional benefits of food. Fermented food may interfere with pathogen infections through a variety of mechanisms, including competitive exclusion or improving intestinal barrier integrity. In this study, the effect of milk fermented with Lactococcus lactis ssp. cremoris JFR1 on Salmonella invasion of intestinal epithelial cell cultures was investigated. Epithelial cells (HT29-MTX, Caco-2, and cocultures of the 2) were treated for 1 h with Lactococcus lactis ssp. cremoris JFR1 fermented milk before infection with Salmonella enterica ssp. enterica Typhimurium. Treatment with fermented milk resulted in increased transepithelial electrical resistance, which remained constant for the duration of infection (up to 3 h), illustrating a protective effect. After gentamicin treatment to remove adhered bacterial cells, enumeration revealed a reduction in numbers of intracellular Salmonella. Quantitative reverse-transcription PCR data indicated a downregulation of Salmonella virulence genes hilA, invA, and sopD after treatment with fermented milk. Fermented milk treatment of epithelial cells also exhibited an immunomodulatory effect reducing the production of proinflammatory IL-8. In contrast, chemically acidified milk (glucono delta-lactone) failed to show the same effect on monolayer integrity, Salmonella Typhimurium invasion, and gene expression as well as immune modulation. Furthermore, an oppA knockout mutant of Salmonella Typhimurium infecting treated epithelial cells did not show suppressed virulence gene expression. Collectively, these results suggest that milk fermented with Lactococcus lactis ssp. cremoris JFR1 is effective in vitro in the reduction of Salmonella invasion into intestinal epithelial cells. A functional OppA permease in Salmonella is required to obtain the antivirulence effect of fermented milk.

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“…(2) Higher secretion of IgA due to probiotic feeding has been reported in mice by Kemgang et al . 20 . (3) In addition to the works by Dykstra et al .…”

Section: Discussionmentioning

confidence: 99%

Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains

Plaza‐Díaz

Robles-Sánchez

Abadía-Molina

et al. 2017

Sci Rep

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We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Leprfa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Leprfa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secretory IgA content in the gut and lipopolysaccharide (LPS)-binding protein (LBP) in serum. Expression of three genes (Adamdec1, Ednrb and Ptgs1/Cox1) was up-regulated in the intestinal mucosa of the obese rats compared with that in the rats when they were still lean. Probiotic administration down-regulated expression of Adamdec1 and Ednrb at the mRNA and protein levels and that of Ptgs1/Cox1 at the mRNA level, and this effect was in part mediated by a decrease in both macrophage and dendritic cell populations. Probiotic treatment also increased secretory IgA content and diminished the LBP concentration. Based on results reported in this work and else where, we propose a possible mechanism of action for these bacterial strains.

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Fermented milk with probiotic Lactobacillus rhamnosus S1K3 (MTCC5957) protects mice from salmonella by enhancing immune and nonimmune protection mechanisms at intestinal mucosal level

Cited by 34 publications

References 92 publications

Mixed Lactobacillus plantarum Strains Inhibit Staphylococcus aureus Induced Inflammation and Ameliorate Intestinal Microflora in Mice

Mixed Lactobacillus plantarum Strains Inhibit Staphylococcus aureus Induced Inflammation and Ameliorate Intestinal Microflora in Mice

Effect of fermented milk from Lactococcus lactis ssp. cremoris strain JFR1 on Salmonella invasion of intestinal epithelial cells

Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains

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