Femtochemistry and Femtobiology: The New Age

Local structure and dynamics of hemeproteins by X-ray absorption near edge structure spectroscopy

“…radiation damage, low signal to noise ratio, ultrafast data acquisition) must be overcome along this perspective as in the case of the "single molecule" X-ray diffraction experiment, which is under development at the X-FEL laboratories [61,62]. Thus, in the near future, the possibility to monitor subtle differences due to light induced or X-ray induced changes into the metal center of a protein, will put XANES spectroscopy applied to biological samples, even more into the hot spot [63].…”

Local structure and dynamics of hemeproteins by X-ray absorption near edge structure spectroscopy

“…radiation damage, low signal to noise ratio, ultrafast data acquisition) must be overcome along this perspective as in the case of the "single molecule" X-ray diffraction experiment, which is under development at the X-FEL laboratories [61,62]. Thus, in the near future, the possibility to monitor subtle differences due to light induced or X-ray induced changes into the metal center of a protein, will put XANES spectroscopy applied to biological samples, even more into the hot spot [63].…”

Ligand Binding Intermediates of Nitrosylated Human Hemoglobin Induced at Low Temperature by X-ray Irradiation

“…In conclusion, our paper, focused on the finding of X-ray induced photolysis, also shows the potential of XANES to reveal, and to distinguish between, intermediate states of hemoglobin. Even if our experimental conditions are far from physiological, the results give a glimpse for the interpretation of future time-resolved XAS investigations, done by pump–probe experiments using synchrotron or X-FEL sources, that could be done at room temperature, ,, being able to discern protein dynamics events related to an heterogeneous system of multiple heme subunits.…”

Ultrafast X-ray Absorption Spectroscopy

“…We mention this example here not only for historical reasons but also because Mössbauer spectroscopy is an extremely powerful tool used in structural research, which may even be viewed as an analogue to XAS in nuclear spectroscopy. We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics …”

“…We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics. 167 In the following, we review the studies that were carried out using the pulsed nature of the X-ray source, descending from the microsecond time domain to the femtosecond time scale.…”

Local structure and dynamics of hemeproteins by X-ray absorption near edge structure spectroscopy

“…radiation damage, low signal to noise ratio, ultrafast data acquisition) must be overcome along this perspective as in the case of the "single molecule" X-ray diffraction experiment, which is under development at the X-FEL laboratories [61,62]. Thus, in the near future, the possibility to monitor subtle differences due to light induced or X-ray induced changes into the metal center of a protein, will put XANES spectroscopy applied to biological samples, even more into the hot spot [63].…”

Ligand Binding Intermediates of Nitrosylated Human Hemoglobin Induced at Low Temperature by X-ray Irradiation

“…In conclusion, our paper, focused on the finding of X-ray induced photolysis, also shows the potential of XANES to reveal, and to distinguish between, intermediate states of hemoglobin. Even if our experimental conditions are far from physiological, the results give a glimpse for the interpretation of future time-resolved XAS investigations, done by pump–probe experiments using synchrotron or X-FEL sources, that could be done at room temperature, ,, being able to discern protein dynamics events related to an heterogeneous system of multiple heme subunits.…”

Ultrafast X-ray Absorption Spectroscopy

“…We mention this example here not only for historical reasons but also because Mössbauer spectroscopy is an extremely powerful tool used in structural research, which may even be viewed as an analogue to XAS in nuclear spectroscopy. We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics …”

“…We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics. 167 In the following, we review the studies that were carried out using the pulsed nature of the X-ray source, descending from the microsecond time domain to the femtosecond time scale.…”

Local structure and dynamics of hemeproteins by X-ray absorption near edge structure spectroscopy

“…radiation damage, low signal to noise ratio, ultrafast data acquisition) must be overcome along this perspective as in the case of the "single molecule" X-ray diffraction experiment, which is under development at the X-FEL laboratories [61,62]. Thus, in the near future, the possibility to monitor subtle differences due to light induced or X-ray induced changes into the metal center of a protein, will put XANES spectroscopy applied to biological samples, even more into the hot spot [63].…”

Ligand Binding Intermediates of Nitrosylated Human Hemoglobin Induced at Low Temperature by X-ray Irradiation

“…In conclusion, our paper, focused on the finding of X-ray induced photolysis, also shows the potential of XANES to reveal, and to distinguish between, intermediate states of hemoglobin. Even if our experimental conditions are far from physiological, the results give a glimpse for the interpretation of future time-resolved XAS investigations, done by pump–probe experiments using synchrotron or X-FEL sources, that could be done at room temperature, ,, being able to discern protein dynamics events related to an heterogeneous system of multiple heme subunits.…”

Ultrafast X-ray Absorption Spectroscopy

“…We mention this example here not only for historical reasons but also because Mössbauer spectroscopy is an extremely powerful tool used in structural research, which may even be viewed as an analogue to XAS in nuclear spectroscopy. We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics …”

“…We feel its extension to time-resolved studies, especially in the short time domain, could open a new area of research in structural dynamics. 167 In the following, we review the studies that were carried out using the pulsed nature of the X-ray source, descending from the microsecond time domain to the femtosecond time scale.…”