Female Reproductive Success Variation in a Pseudotsuga menziesii Seed Orchard as Revealed by Pedigree Reconstruction from a Bulk Seed Collection

El‐Kassaby, Yousry A.; Funda, Tomáš; Lai, Ben S. K.

doi:10.1093/jhered/esp126

Cited by 25 publications

(23 citation statements)

References 39 publications

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“…When the actual gametic contribution is known, then seed crops' genetic worth (represented by average breeding values weighted by parental gametic contribution) and genetic diversity (represented by effective population size) are precisely estimated (Stoehr et al 1998;Slavov et al 2005;El-Kassaby et al 2010). Gene flow within seed orchard populations is affected by three main factors; namely, 1) females' phenological asynchrony and males' fertility variation, 2) level of gene flow from external pollen sources, and 3) level of self-fertilization (Burczyk et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysis

Korecký¹,

El‐Kassaby²

2016

Silva Fenn.

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Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysisKorecký J., El-Kassaby Y.A. (2016). Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysis. Silva Fennica vol. 50 no. 4 article id 1682. 12 p. http:// dx.doi.org/10.14214/sf.1682. Highlights• Important characteristics such as parental reproductive success, pollen contamination, and selfing rate in the second generation Douglas-fir seed orchard have been estimated. • Since this research is a part of a multi-year study, outputs were compared to those from two other years.• Results are in line and differences in pollination dynamics across years are attributable to the various crop management practices. AbstractAs part of a multi-year monitoring study of pollination dynamics in a second generation Douglasfir (Pseudotsuga menziesii (Mirb.) Franco) seed orchard, we estimated with the aid of eight microsatellite markers three important reproductive biology characteristics affecting the genetic worth and diversity of seed crops; namely parental reproductive success, pollen contamination, and selfing rate. The obtained results were compared to those from two previous years to gauge appropriate seed crop management practices and ultimately allow approximate generalization of seed crop genetic quality. We determined that 80% of parental gametes were produced by 52% of the parents, 13% of paternal gametes resulted from pollen contamination (i.e., gene flow), and 12% of the seed were the product of selfing. The obtained results were in line with those observed for 2005 and 2009 where 80% of gametes being produced by 37-48% of the parents, 10-18% pollen contamination, and 15-17% selfing rate. The observed reproductive biology parameters differences are attributable to the various crop management practices implemented (i.e., bloom delay and supplemental-mass-pollination) across years and calls for justification due to the observed minimal differences on seed crops genetic quality.

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Section: Introductionmentioning

confidence: 99%

Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysis

Korecký¹,

El‐Kassaby²

2016

Silva Fenn.

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“…Given the paternal inheritance of chloroplast in conifers, cp-DNA microsatellites were also used for the same purpose (Plomion et al 2001;Stoehr et al 1998Stoehr et al , 2006. With a wider access to highly multi-allelic nuclear microsatellites, these became the markers of choice for conifers to study contamination rates in seed orchards of conifer species such Maritime pine (Pinus pinaster) (Fernandes et al 2008), Cryptomeria japonica (Moriguchi et al 2004), and Douglas Fir (Pseudotsuga menziesii) (Slavov et al 2005a); assess the outcome of SMP in Douglas Fir ; reconstruct pedigrees to correct breeding values in maritime pine (Gaspar et al 2009) and red spruce (Picea rubens) (Doerksen and Herbinger 2010;El-Kassaby et al 2010); and carry out "Breeding-without-Breeding" strategies in Douglas Fir and Larch (Larix occidentalis) (El-Kassaby et al 2011;ElKassaby and Lstiburek 2009) and Scots Pine (Pinus sylvestris) .…”

Section: Introductionmentioning

confidence: 99%

Performance of microsatellites for parentage assignment following mass controlled pollination in a clonal seed orchard of loblolly pine (Pinus taeda L.)

Grattapaglia

Diener

Santos

2014

Tree Genetics & Genomes

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Mass controlled pollination (MCP), involving large-scale application of pollen on physically isolated female reproductive organs, has been a lower cost alternative to controlled pollination for the commercial production of genetically improved seeds. Nevertheless, rare are the studies that examined the efficacy of operational MCP and no such assessment has been done in loblolly pine to date. The success of MCP was assessed by a microsatellite-based investigation of the realized versus expected parentage of a set of 300 Pinus taeda offspring in 19 families generated in two subsequent rounds of MCP in 2005 and 2006 in a clonal seed orchard in Brazil. Multi-locus combined probability of parentage exclusion both theoretical and realized from actual testing was >99 % for single parent and parent pair testing when using nine or ten markers. Parentage assignments carried out under a maximum likelihood framework revealed a significantly higher success rate of MCP in 2006 (84 %) following technical improvements adopted to minimize pollen contamination and maximize male reproductive success, although significant variability in the correct maternity and full parentage was seen among individual families. The observed patterns of unexpected parentage indicated that this variability likely resulted from mislabeling of clonal ramets of the parents used in the crosses which impacted both maternity and paternity. Preventing pollen contamination will not be sufficient for successful MCP if inaccuracies exist in the identity of the clonal plants that ultimately provide pollen and female strobili, showing that DNA marker auditing and correction of identity of all ramets in a clonal seed orchard should be a standard practice in the operational implementation of MCP.

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“…Another study conducted a full and partial reconstruction pedigree of lodgepole pines ( Pinus contorta ) and found that the full pedigree reconstruction was superior to the partial pedigree reconstruction, enabling the estimation of both paternal- and maternal-related fertility parameters [39]. In Douglas fir ( Pseudotsuga menziesii ), pedigree reconstruction was used to identify the maternal and paternal parents of each seed and estimate the clonal reproductive success and selfing rate and determine the proportion of seeds sired by outside pollen sources [40]. Similarly, Funda et al compared the reproductive success of three seed orchards [lodgepole pine, Douglas fir, and western larch ( Larix occidentalis )] based on pedigree reconstructions [41].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of efficiency of controlled pollination based parentage analysis in a Larix gmelinii var. principis-rupprechtii Mayr. seed orchard

Sun

Dong

et al. 2017

PLoS ONE

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Controlled pollination (CP) is an important tool for breeding programs to improve seed quality, as it rapidly generates desirable genotypes and maximizes genetic gains. However, few studies have evaluated the success rate of CP, especially in Larix gmelinii var. principis-rupprechtii Mayr. seed orchards. In this study, we estimated the rate of correct parentage in 257 CP progeny in an L. gmelinii var. principis-rupprechtii seed orchard from ten candidate parents using 13 microsatellites. The parentage exclusion probabilities of all combined loci in the single parent and parent pair tests were > 0.99, which was sufficient to distinguish the relatedness of the sampled individuals. Comparing the maximum likelihood-based parentage analysis results with breeding records revealed that the percentages of correctly identified maternal and paternal parents were 22.6% and 35.0% at 95% CL, respectively, suggestive of parent mislabeling and pollen contamination in the CP population. We conducted a pedigree reconstruction by identifying the expected parents and assigned maternity, paternity, and parent pairs to 176 (68.5%), 199 (77.4%), and 132 (51.4%) progeny, respectively. This study provides a reference for future selection of elite genotypes for commercial production. To increase the efficiency of CP, molecular markers should be used to correctly identify individuals in seed orchards before conducting CP.

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Female Reproductive Success Variation in a Pseudotsuga menziesii Seed Orchard as Revealed by Pedigree Reconstruction from a Bulk Seed Collection

Cited by 25 publications

References 39 publications

Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysis

Pollination dynamics variation in a Douglas-fir seed orchard as revealed by microsatellite analysis

Performance of microsatellites for parentage assignment following mass controlled pollination in a clonal seed orchard of loblolly pine (Pinus taeda L.)

Evaluation of efficiency of controlled pollination based parentage analysis in a Larix gmelinii var. principis-rupprechtii Mayr. seed orchard

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