Feline one-way mixed leukocyte reaction

Stiff, Mary I.; Olsen, Richard G.

doi:10.1016/0165-2427(84)90022-9

Cited by 10 publications

(3 citation statements)

References 4 publications

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“…Allogeneic skin transplantation in cats has been shown to cause the production of cytotoxic antibodies against the donor's lymphocytes [108,109]. Also there is some controversy as to the lack of polymorphism of FLA reported by some workers using mixed lymphocyte reactions [106,107,[110][111][112]. Using gel electrophoresis of homogenized feline lymphocytes followed by Western blot analysis and immunochemical staining with antibodies directed against human class I and II MHC antigens, feline lymphocytes were found to bear determinants that are as polymorphic as human lymphocytes [113].…”

Section: Major Histocompatibility Complexmentioning

confidence: 99%

Feline immune system

Lin

1992

Comparative Immunology, Microbiology and Infectious Diseases

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Section: Major Histocompatibility Complexmentioning

confidence: 99%

Feline immune system

Lin

1992

Comparative Immunology, Microbiology and Infectious Diseases

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“…In contrast to earlier suggestions (Pollack et al 1982), recent reports suggest a considerable level of polymorphism. Others were able to elicit primary mixed lymphocyte reactions (Wolfe et al 1984, Stiff andOlsen, 1984). Others were able to elicit primary mixed lymphocyte reactions (Wolfe et al 1984, Stiff andOlsen, 1984).…”

Section: Introductionmentioning

confidence: 99%

The primary structure of a feline class I gene: Striking similarity toHLA-A

et al. 1989

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The sequence of a feline class I pseudogene and its comparison with class I genes from other species is presented. The gene isolated is a pseudogene because of the presence of four stop codons and two frame shift mutations in the first- and second-domain encoding exons, as well as a mutation in a splice acceptor site in the third intron. By sequence comparison with the other class I sequences determined to date, the FLA pseudogene is most closely related to the HLA-A locus products (88% nucleotide identity.

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“…Early observations that cats show prolonged allograft survival (14)(15)(16), fail to develop lymphocytotoxic alloantibodies after pregnancy or transfusions (13), and succumb to multiple viral infections have led to speculation that cats may have limited or no polymorphism at the MHC (7,13). However, recent reports suggest that domestic cats do have polymorphic class I genes and MHC gene products (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of FLA, the cat major histocompatibility complex.

Winkler¹,

Schultz²,

Cevario³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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The major histocompatibility complex (MHC) of the domestic cat (termed FLA) has been refractile to genetic and serological definition largely because of repeated failure to detect cytotoxic antibodies in multiparous cats or to elicit antibody following allogeneic lymphocyte immunization. We have developed a protocol for producing cytotoxic alloantisera in the cat following rejection of multiple surgical skin grafts. Of 59 cats subjected to grafting, 13 produced lymphocytotoxic antisera which had varying specificities among a panel of outbred cat cells. A population cluster analysis of the 13 alloantisera permitted the identification of six clusters of overlapping FLA specificities. The major histocompatibility complex (MHC) is a multigene cluster in mammals encoding two distinct classes of molecules involved in the presentation of foreign antigen to the T-cell receptor (1-5). Class I genes encode a 45-kDa glycoprotein that is noncovalently linked with an invariant light chain, P2-microglobulin (f82m), on the surfaces of virtually all nucleated cells (4, 6). Class II genes encode the heavy (32-35 kDa) and light (25-28 kDa) subunits of the noncovalently associated af3 heterodimeric glycoproteins expressed on the surfaces of antigen-presenting cells and activated T cells (5). By serological analysis, both class I and class II antigens are abundantly polymorphic in most outbred species. DNA restriction analysis has also revealed extensive polymorphism of the MHC of mice, humans, and several other species (7,8). The function of both class I and class II antigens is to present foreign, often viral, antigen to the T cell receptor. In this way, the cellular immune response is directed to parasitized cells instead of free virus particles (9,10).The domestic cat is an important model for at least two viral diseases of the immune system in humans, human T cell leukemia and acquired immunodeficiency syndrome (AIDS) (11,12). The characterization of the feline MHC as a prerequisite for the study of disease progression has been difficult because of an apparent inability to raise typing alloantisera in the cat (13 MATERIALS AND METHODSDetails of allograft transplantation are described elsewhere (ref. 21; C.W. and S.O., unpublished data). Briefly, split thickness skin grafts were surgically exchanged between selected cats at 21-day intervals. In some cases, intraperitoneal booster injections of peripheral blood lymphocytes (PBLs) from 15 ml of blood were performed at 4-day intervals after grafting (21). Serum was collected from graft cats weekly and used in a cat complement-dependent cytotoxicity assay. A modification of a two-stage assay using 51Cr release as an indicator ofcell death was used to detect alloantibodies against donor cat lymphocytes. The monoclonal antibody W6/32 (23), specific for human class I heavy chain, and the monoclonal antibodies IB5 (human DR and DQ specific) (24) and ISCR3 (mouse I-EK specific) (25) were used as positive controls in immunoprecipitation experiments.Interleukin 2-stimulated or fr...

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Feline one-way mixed leukocyte reaction

Cited by 10 publications

References 4 publications

Feline immune system

Feline immune system

The primary structure of a feline class I gene: Striking similarity toHLA-A

Genetic characterization of FLA, the cat major histocompatibility complex.

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