Feline immune system

Lin, Dah-Sheng

doi:10.1016/0147-9571(92)90097-b

Cited by 12 publications

(6 citation statements)

References 98 publications

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“…However, the FIV-cat system, being the smallest natural model of lentivirus infection, has a number of logistical and practical assets that should not be overlooked (Table 6), and as discussed above, has already proven to be a powerful tool for deciphering the biology, pathogenesis, and susceptibility to drugs and vaccine-induced immunity of immunodeficiency-inducing lentiviruses. The only real logistical drawbacks of studies with FIV are a shortage of immunological reagents and the relatively poor knowledge of the immune system of cats (114). These two shortcomings are rapidly changing (252) and could be overcome by appropriate fostering of FIV research.…”

Section: Discussionmentioning

confidence: 99%

Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

Bendinelli

Pistello

Lombardi

et al. 1995

Clinical Microbiology Reviews

141

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Section: Discussionmentioning

confidence: 99%

Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

Bendinelli

Pistello

Lombardi

et al. 1995

Clinical Microbiology Reviews

141

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“…The rhesus monkey, widely used as a model to study infection and immunity (5,6) due to its genetic relationship with humans, is used for the development and testing of vaccines as is the rabbit (7), although evolutionarily closer to mouse than to human. The cat is for example a model for the study of the immunodeficiency virus due to the similarities between the feline immunodeficiency virus and the human one (8,9), and the dog is a reliable model for the immune response during the development (10,11). The ferret is an animal model of predilection for the pathogenesis of different respiratory viruses (12) as it has a lung physiology similar to that of human (13).…”

Section: Introductionmentioning

confidence: 99%

IMGT® Biocuration and Comparative Study of the T Cell Receptor Beta Locus of Veterinary Species Based on Homo sapiens TRB

et al. 2020

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IMGT ® , the international ImMunoGeneTics information system ® is the global reference in immunogenetics and immunoinformatics. By its creation in 1989 by Marie-Paule Lefranc (Université de Montpellier and CNRS), IMGT ® marked the advent of immunoinformatics, which emerged at the interface between immunogenetics and bioinformatics. IMGT ® is specialized in the immunoglobulins (IG) or antibodies, T cell receptors (TR), major histocompatibility (MH), and proteins of the IgSF and MhSF superfamilies. T cell receptors are divided into two groups, αβ and γ δ TR, which express distinct TR containing either α and β, or γ and δ chains, respectively. The TRβ locus (TRB) was recently described and annotated by IMGT ® biocurators for several veterinary species, i.e., cat (Felis catus), dog (Canis lupus familiaris), ferret (Mustela putorius furo), pig (Sus scrofa), rabbit (Oryctolagus cuniculus), rhesus monkey (Macaca mulatta), and sheep (Ovis aries). The aim of the present study is to compare the genes of the TRB locus among these different veterinary species based on Homo sapiens. The results reveal that there are similarities but also differences including the number of genes by subgroup which may demonstrate duplications and/or deletions during evolution.

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“…Immunoglobulin G (IgG) is a class of antibodies produced by immune cells in response to infections. The level of IgG is commonly targeted for the detection of feline infectious diseases and is widely utilized in commercial detection kits available globally . Herein, enrichment selection of a single-chain variable fragment was performed to select feline IgG-bound scFv for biosensor development.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the development of a rapid detection kit remains essential and challenging. The IgG level is typically targeted for feline infectious detection and has been incorporated into commercial detection kits available worldwide . Monoclonal antibodies specific to feline IgG are vital components in feline detection kits.…”

Section: Introductionmentioning

confidence: 99%

“…The IgG level is typically targeted for feline infectious detection and has been incorporated into commercial detection kits available worldwide. 2 Monoclonal antibodies specific to feline IgG are vital components in feline detection kits. Some feline infectious disease detection kits utilize the IgG response for diagnosing diseases, such as feline immunodeficiency virus (FIV) 3 , 4 and toxoplasmosis.…”

Section: Introductionmentioning

confidence: 99%

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Generation of a Single-Chain Variable Fragment Antibody against Feline Immunoglobulin G for Biosensor Applications

et al. 2023

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For many decades, feline infectious disease has been among the most common health problems and a leading cause of death in cats. These diseases include toxoplasmosis, feline leukemia virus (FeLV), and particularly feline immunodeficiency virus (FIV) disease. Early diagnosis is essential to increase the chance of successful treatment. Generally, measurement of the IgG level is considered to be indicative of an individual’s immune status for a particular pathogen. The antibodies specific to feline IgG are crucial components for the development of a detection kit. In this study, feline IgG-bound scFv was selected using phage display technology. Three rounds of biopanning were conducted against purified feline IgG. Through an indirect enzyme-linked immunosorbent assay (ELISA), two scFv clones demonstrating the best binding ability to feline IgG were chosen for biochemical characterization. In addition, the selected scFv (N14) was expressed and purified in a bacterial system. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the size of the purified N14 was 29 kDa. A sandwich ELISA was used to evaluate the binding capacity of the purified scFv to feline IgG. As expected, the purified N14 had the capacity to bind feline IgG. Furthermore, N14 was modified to create a scFv-alkaline phosphatase (scFv-AP) fusion platform. The surface plasmon resonance (SPR) results revealed that N14-AP bound to feline IgG with an affinity binding value of 0.3 ± 0.496 μM. Additionally, the direct ELISA demonstrated the binding capacity of N14-AP to feline IgG in both cell lysate and purified protein. Moreover, N14-AP could be applied to detect feline IgG based on electrosensing with a detection limit of 10.42 nM. Overall, this study successfully selected a feline IgG-bound scFv and developed a scFv-AP platform that could be further engineered and applied in a feline infectious disease detection kit.

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Feline immune system

Cited by 12 publications

References 98 publications

Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

IMGT® Biocuration and Comparative Study of the T Cell Receptor Beta Locus of Veterinary Species Based on Homo sapiens TRB

Generation of a Single-Chain Variable Fragment Antibody against Feline Immunoglobulin G for Biosensor Applications

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