Felid herpesvirus 1 glycoprotein G is a structural protein that mediates the binding of chemokines on the viral envelope

Alphaherpesviruses and Chemokines: Pas de Deux Not Yet Brought to Perfection

“…The FeHV-1 gG homologue may be a determinant for cell and tissue tropism in vivo and/or may aid in virus entry. Although it has been shown that FeHV-1 gG can act as a vCKBP when present on the virion surface, preincubation of virions with chemokines, including CXCL1, CCL3, or XCL1, did not alter the infectivity of FeHV-1, and these data would therefore not support a role for gG in cell and tissue tropism in the chosen in vitro system (20). However, a cell-type-specific interaction between FeHV-1 gG and GAG-bound chemokines on target cells is easily conceivable.…”

“…It is possible that membrane-bound gG acts as a bona fide viroreceptor, providing a decoy that prevents the interaction of chemokines with cellular receptors and inhibits the biological activity of chemokines. In addition, FeHV-1 gG is a structural protein and is present on the surfaces of virus particles (20). This observation begs the speculation that membrane-bound gG, besides functioning as a viroreceptor, might also play a role in virus attachment to cells which present chemokines bound to GAGs.…”

Cloning of the Koi Herpesvirus Genome as an Infectious Bacterial Artificial Chromosome Demonstrates That Disruption of the Thymidine Kinase Locus Induces Partial Attenuation in Cyprinus carpio koi

“…Next, we tried to clone circular intermediates of the FL BAC genome into bacteria by classical approaches that we have used successfully in the past for the BAC cloning of other herpesviruses (9,11,16). Surprisingly, despite the screening of more than 500 independent clones, we were unable to select a single clone carrying the FL BAC genome.…”

“…After an incubation period of 2 h, cells were washed with phosphate-buffered saline and then overlaid with Dulbecco's modified essential medium (Invitrogen) containing 4.5 g of glucose/liter and 10% FCS. The supernatants of infected cultures were harvested at successive intervals after infection, and the amount of infectious virus was determined by plaque assays with CCB cells as described previously (9).…”

The Major Portal of Entry of Koi Herpesvirus in Cyprinus carpio Is the Skin

“…After an incubation period of 2 h, the cells were washed with PBS and then overlaid with Dulbecco's modified essential medium (DMEM) (Invitrogen) containing 4.5 g/liter glucose and 10% FCS. The supernatants of infected cultures were harvested at successive intervals after infection, and the amount of infectious virus was determined by plaque assay on CCB cells as described previously (5).…”