Feedback Control of the Arachidonate Cascade in Rheumatoid Synoviocytes by 15-deoxy-Δ12,14-Prostaglandin J2

15‐Deoxy‐Δ12,14‐prostaglandin J2 inhibitsCX3CL1/fractalkine expression in human endothelial cells

“…[28][29][30] We found that ciglitazone, another PPAR-γ agonist, had no effect on the cytokineinduced fractalkine expression; and a specific PPAR-α agonist WY14643 was also inert. Thus 15d-PGJ 2 may inhibit the endothelial fractalkine expression in a manner independent of PPAR.…”

Sphingosine 1‐phosphate/sphingosine 1‐phosphate receptor 1 signaling in rheumatoid synovium: Regulation of synovial proliferation and inflammatory gene expression

“…RA synoviocytes or MH7A cells (2 ϫ 10 4 ) were plated in flat-bottomed 24-well microplates and were cultured with S1P (0-0.1 M) in the presence or absence of 100 ng/ml TNF␣ (Sigma) or 10 pg/ml IL-1␤ (Sigma). After 72 hours of incubation, PGE 2 synthesis was determined by assaying supernatants with a PGE 2 enzymelinked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (Assay Designs, Ann Arbor, MI) (33).…”

Molecular Basis for the Direct Inhibition of AP-1 DNA Binding by 15-Deoxy-Δ12,14-prostaglandin J2

“…cyPG have received considerable attention during the past decade due to their action as antiproliferative agents and inducers of apoptosis in a variety of normal and cancer cell types (6,7). cyPG also display anti-inflammatory effects both in cellular and in animal models (2,8,9). This has led to the proposal that cyPG, and in particular 15d-PGJ 2 , may contribute to the resolution of inflammation through various mechanisms that include the inhibition of the expression of pro-inflammatory genes, the induction of apoptosis in activated macrophages, and the activation of the transcription factor peroxisome proliferator activated receptor ␥ (PPAR␥) (10,11).…”