Feed Restriction Significantly Alters Lipogenic Gene Expression in Broiler Breeder Chickens

Abstract

Broiler breeder pullets were divided into two groups at 21 wk of age. One group was given free access to feed (ad libitum) and the other fed a limited amount of feed (restricted). At 22 wk, all birds were photostimulated and maintained throughout an egg-laying cycle ending at 36 wk. Samples of liver and abdominal fat pad were collected just before photostimulation (prelight), after photostimulation at first egg and at peak egg production (plateau). Hepatic expression of sterol regulatory element binding protein-1, ATP-citrate lyase, fatty acid synthase, malic enzyme, acetyl-CoA carboxylase and stearoyl-CoA (Delta9) desaturase 1 genes in ad libitum birds declined from their highest levels just before photostimulation as the birds came into and maintained egg production. In contrast, the restricted birds had significant (P < 0.05) increases in the expression of these genes after photostimulation at first egg with a subsequent decline as they reached peak egg production. Hepatic expression of fatty acid binding protein, VLDL apolipoprotein (apoVLDL-II) and apoB genes increased significantly (P < 0.05) in both ad libitum and restricted breeders after photostimulation, whereas apoA1 gene expression declined during this time. Abdominal fat pad weights were significantly (P < 0.05) higher in the ad libitum compared with restricted birds after photostimulation. Lipoprotein lipase in this tissue showed a pattern of expression similar to that observed for the hepatic lipogenic enzyme genes. In conclusion, feed restriction during the pullet-to-breeder transition period significantly (P < 0.05) altered hepatic lipogenic gene expression in broiler breeders.

Changes in fat metabolism of black-bone chickens during early stages of infection with Newcastle disease virus

“…Moreover, the changes in protein levels of PPARg, FAS and ATGL were basically consistent with the changes in mRNA expression. It has been found that changes in feed intake had significant effects on genes expression in the animal for a long time (Richards et al, 2003;Sun et al, 2006). However, NDV infection has more quick and severe changes on gene expression in our study.…”

Microarray analysis of genes differentially expressed in the liver of lean and fat chickens

“…In birds, lipogenesis (the conversion of glucose to TGs) takes place primarily in the liver, whereas the adipocyte serves as the storage site for TGs (Cogburn et al, 2004). Hepatic lipogenesis plays a direct role in the accumulation of lipid in the avian adipocyte, and 80% to 85% of the FAs stored in adipose tissue are generated through lipogenesis in the liver or from the diet (Richards et al, 2003).…”

Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues

“…In fat tissues, glucose and other nutrients are converted into fatty acids by the FASregulated pathway (Richards et al, 2003). Therefore, overexpression of FAS would increase triglyceride deposition in the body, which may cause obesity (Richards et al, 2003;Matsubara et al, 2005).…”