Fecal microflora of Greek healthy neonates

Mitsou, Evdokia K.; Kirtzalidou, Ekaterini; Oikonomou, Ioannis; Liosis, George; Kyriacou, Adamantini

doi:10.1016/j.anaerobe.2007.11.002

Cited by 74 publications

(79 citation statements)

References 36 publications

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“…The above results demonstrated that Clostridium butyrium affects offspring through breast milk, while having a direct effect when orally administered after weaning. This is in accordance with the findings of a previous study on breast-fed subjects, which reported that infants were able to acquire probiotics via breast milk (17). Clostridium butyrium does not colonize in the normal intestinal flora and keeps a balance of ## P<0.01 vs. Ba; ++ P<0.01 vs. Bb.…”

Section: Discussionsupporting

confidence: 82%

Effect of Clostridiumï¿½butyricum supplementation on the development of intestinal flora and the immune system of neonatal mice

et al. 2017

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Abstract. The objective of the present study was to examine whether Clostridium butyricum supplementation has a role in the regulation of the intestinal flora and the development of the immune system of neonatal mice. A total of 30 pregnant BALB/c mice, including their offspring, were randomly divided into three groups: In the maternal intervention group (Ba), maternal mice were treated with Clostridium butyricum from birth until weaning at postnatal day 21 (PD21) followed by administration of saline to the offspring at PD21-28; in the offspring intervention group (Ab), breast-feeding maternal mice were supplemented with saline and offspring were directly supplemented with Clostridium butyricum from PD21-28; in the both maternal and offspring intervention group (Bb), both maternal mice and offspring were supplemented with Clostridium butyricum at PD 0-21 and at PD21-28. While mice in the control group were given the same volume of normal saline. Stool samples from the offspring were collected at PD14, -21 and -28 to observe the intestinal flora by colony counts of Enterococcus spp., Enterobacter spp., Bifidobacterium spp. and Lactobacillus spp. Detection of intestinal secreted immunoglobulin A (sIgA) levels and serum cytokine (interferon-γ, and interleukin-12, -4 and -10) levels in offspring was performed to evaluate the effect on their immune system. The results revealed that compared with the control group, offspring in the Ba group displayed significantly decreased stool colony counts of Enterococcus spp. (t=3.123, P<0.01) at PD14 and significantly decreased counts of Enterobacter spp. at P<0.05 and t=2.292, P<0.05, respectively). Compared with the control group, the stool colony counts of Bifidobacterium spp. and Lactobacillus spp. were significantly increased in the Ba group at PD21 (t=3.085, P<0.01 and t=2.8508, P<0.05, respectively). The Ab group had significantly higher stool colony counts of Bifidobacterium spp. and Lactobacillus spp. at PD28, compared with the control group (Q=7.679, P<0.01 and Q=6.149, P<0.01, respectively). There were no significant differences identified in the sIgA levels of the intestinal fluid and serum cytokine levels between the control group and the intervention groups. In conclusion, Clostridium butyricum administered to breast-feeding maternal mice was able to regulate the intestinal flora balance in their offspring. However, due to insignificant effects on sIgA level and the associated cytokines, Clostridium butyricum had a limited influence on the balance of type 1 vs. type 2 T-helper cells. However, using Clostridium butyricum as an invention may be a safe method for improving the balance of intestinal flora and associated processes in offspring.

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Section: Discussionsupporting

confidence: 82%

Effect of Clostridiumï¿½butyricum supplementation on the development of intestinal flora and the immune system of neonatal mice

et al. 2017

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“…Previous studies found these bacteria in two thirds of the subjects (36,37). However, those studies investigated the situation at the third and fourth day of life (36,37), which might have given the enterococci time to colonize and multiply to a higher degree. Enterococci can be contaminants from the environment at the outpatient accommodation facility and may not necessarily originate from the mother.…”

Section: Discussionmentioning

confidence: 82%

“…Enterococci are opportunistic pathogens causing, for example, nosocomial infections (35). Previous studies found these bacteria in two thirds of the subjects (36,37). However, those studies investigated the situation at the third and fourth day of life (36,37), which might have given the enterococci time to colonize and multiply to a higher degree.…”

Section: Discussionmentioning

confidence: 87%

The Pioneer Gut Microbiota in Human Neonates Vaginally Born at Term—A Pilot Study

et al. 2011

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“…The interaction between host and gut microbiome starts right from birth and continues throughout the life. The microbiota colonize the gut soon after birth and the mother of the host is the first source for these gut microbiota [5,6]. A number of factors, including diet [7][8][9], age [10], gender, genetic composition [11], geographic location [2], and health/disease status [6] of the individual influence the gut microbiota after birth.…”

Section: Introductionmentioning

confidence: 99%

Gut microbiome diversity in acute infective and chronic inflammatory gastrointestinal diseases in North India

et al. 2016

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The disease profile in the Indian population provides a unique opportunity for studying the host microbiome interaction in both infectious (amebiasis) and autoimmune diseases like inflammatory bowel disease (IBD) from a similar environment and genetic background. Analysis of fecal samples from untreated amebic liver abscess (ALA) patients, Entamoeba histolytica (Eh)-negative and -positive asymptomatic individuals, and pus samples from naive ALA patients revealed a significant reduction in Lactobacillus in asymptomatic individuals (Eh ?ve) and ALA patients. Two anaerobic genera, namely Bacteroides and Peptostreptococcus, were detected in naive ALA pus samples. Analysis of fecal samples from amoebic colitis patients showed a significant decline in population of Bacteroides, Clostridium coccoides and leptum subgroup, Lactobacillus, Campylobacter, and Eubacterium, whereas a significant increase in Bifidobacterium was observed. Mucosa-associated bacterial flora analysis from IBD patients and healthy controls revealed a significant difference in concentration of bacteria among predominating and subdominating genera between ulcerative colitis (UC), Crohn's disease (CD) patients, and controls. In contrast to the mucosal studies, we found a significant increase in lactobacilli population in fecal samples of active UC patients. Another study revealed a significant decrease of Clostridium coccoides and leptum clusters in fecal samples of active UC patients along with decreased concentrations of fecal SCFAs, especially of nbutyrate, iso-butyrate, and acetate. We therefore found similar perturbations in gut microbiome in both infectious and autoimmune diseases, indicating inflammation to be the major driver for changes in gut microbiome.

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Fecal microflora of Greek healthy neonates

Cited by 74 publications

References 36 publications

Effect of Clostridiumï¿½butyricum supplementation on the development of intestinal flora and the immune system of neonatal mice

Effect of Clostridiumï¿½butyricum supplementation on the development of intestinal flora and the immune system of neonatal mice

The Pioneer Gut Microbiota in Human Neonates Vaginally Born at Term—A Pilot Study

Gut microbiome diversity in acute infective and chronic inflammatory gastrointestinal diseases in North India

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