An iron density profile is accurately determined in Large Helical Device (LHD) using a space-resolved extreme ultraviolet (EUV) spectrometer, the absolute intensity calibration of which is carried out by bremsstrahlung continuum measurement. The effective intensity coefficients R (eV cm3 s-1) of FeXV to FeXXIV are precisely calculated on the basis of a collisional-radiative model for iron density determination. The total iron density at the plasma center is found to be almost 4 or 5 orders of magnitude smaller than the electron density. The application of the present result to the study of impurity transport demonstrates a new way of examining the radial structure of transport coefficients and of determining the total impurity density.