Fe Sparing and Fe Recycling Contribute to Increased Superoxide Dismutase Capacity in Iron-Starved <i>Chlamydomonas reinhardtii</i>

Page, M. Dudley; Allen, Michael D.; Kropat, Janette; Urzica, Eugen I.; Karpowicz, Steven J.; Hsieh, Scott I.; Loo, Joseph A.; Merchant, Sabeeha

doi:10.1105/tpc.112.098962

Cited by 82 publications

(92 citation statements)

References 129 publications

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“…2). As observed previously (20,40), iron starvation results in a rapid decrease in ferredoxin in the first 24 h, whereas CTH1, a diiron-containing enzyme was more slowly reduced. When the levels of FAB2 were monitored, we detected a similar profile as seen for ferredoxin with a dramatic decrease in the enzyme abundance in the first 24 h of iron starvation ( Fig.…”

Section: Resultssupporting

confidence: 60%

“…Several studies, including the present one, demonstrated that under iron limitation and iron deprivation conditions, ferredoxin abundance decreases in C. reinhardtii (22,23,27,40), suggesting that combined defects in all the iron-dependent enzymes required for FA desaturation have a negative impact on polyunsaturated FA levels. Noticeably, our study showed that ferredoxin and at least one FA desaturase (FAB2) have a similar pattern of protein expression and both have diminished abundance starting at 8 -12 h of iron starvation.…”

Section: Iron Starvation Has a Major Impact On Glycerolipid Content Imentioning

confidence: 99%

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Remodeling of Membrane Lipids in Iron-starved Chlamydomonas

Urzica¹,

Vieler

Hong-Hermesdorf³

et al. 2013

Journal of Biological Chemistry

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Background: Iron starvation triggers lipid droplet and triacylglycerol (TAG) accumulation in Chlamydomonas reinhardtii. Results: The overall lipid profile shows a decrease in the absolute content of monogalactosyldiacylglycerol (MGDG) and an increase in saturated and monounsaturated fatty acids. Conclusion: Iron starvation has an early and distinct effect on membrane lipids, before onset of chlorosis. Significance: Iron deficiency affects distribution of lipid type as well as fatty acid profile.

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Section: Resultssupporting

confidence: 60%

Section: Iron Starvation Has a Major Impact On Glycerolipid Content Imentioning

confidence: 99%

Remodeling of Membrane Lipids in Iron-starved Chlamydomonas

Urzica¹,

Vieler

Hong-Hermesdorf³

et al. 2013

Journal of Biological Chemistry

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“…Where tested, most of the highly regulated genes (FOX1, FTR1, FEA1, and MSD3) are controlled at the transcriptional level (Allen et al, 2007a;Page et al, 2012). Deletion analysis identified candidate iron response elements (FeREs) in the 59 flanking regions of the FOX1, FTR1, FEA1, and ATX1 genes (Deng and Eriksson, 2007;Fei and Deng, 2007;Fei et al, 2009Fei et al, , 2010.…”

Section: Transcriptional Regulation Of Iron Deficiency Responsesmentioning

confidence: 99%

Systems and Trans-System Level Analysis Identifies Conserved Iron Deficiency Responses in the Plant Lineage

et al. 2012

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“…However, the 1.4-to 1.9-fold upregulation of superoxide dismutases FSD1, MSD1, and MSD2, targeted to chloroplast, cytosol, and mitochondria, respectively (Page et al, 2012), also suggests the increased generation of superoxide (O 2 2 ) under long-term HS. As the dismutases convert superoxide to H 2 O 2 , another source for the obviously increased accumulation of H 2 O 2 might also be superoxide generated, e.g., during photosynthetic LEF by the Mehler reaction at PSI or in the mitochondrial respiratory chain at complexes I and III (Mehler, 1951;Turrens, 1997;Asada, 2006).…”

Section: Decreased Electron Sinks Under Long-term Hs Appears To Incrementioning

confidence: 99%

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

et al. 2014

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We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42°C for 24 h and back to 25°C for ‡8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.

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Fe Sparing and Fe Recycling Contribute to Increased Superoxide Dismutase Capacity in Iron-Starved Chlamydomonas reinhardtii

Cited by 82 publications

References 129 publications

Remodeling of Membrane Lipids in Iron-starved Chlamydomonas

Remodeling of Membrane Lipids in Iron-starved Chlamydomonas

Systems and Trans-System Level Analysis Identifies Conserved Iron Deficiency Responses in the Plant Lineage

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

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