Fc Receptor-Mediated Immunity Against <i>Bordetella pertussis</i>

Rodrı́guez, Marı́a Eugenia; Hellwig, Sandra M.M.; Hozbor, Daniela Flavia; Leusen, Jeanette H.W.; Pol, W. Ludo van der; Winkel, Jan G. J. van de

doi:10.4049/jimmunol.167.11.6545

Cited by 60 publications

(66 citation statements)

References 52 publications

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“…However, results from previous studies using similar OPA protocols and PMNs as effector cells indicate that the internalized B. pertussis bacteria are readily killed in this process (23,25,33). In addition to OPA, the direct complement killing of the bacteria also may play a role.…”

Section: Discussionmentioning

confidence: 62%

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Aase¹,

Herstad²,

Merino³

et al. 2011

Clin. Vaccine Immunol.

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Waning vaccine-induced immunity against Bordetella pertussis is observed among adolescents and adults. A high incidence of pertussis has been reported in this population, which serves as a reservoir for B. pertussis. A fifth dose of reduced antigen of diphtheria-tetanus-acellular-pertussis and inactivated polio vaccine was given as a booster dose to healthy teenagers. The antibody activity against B. pertussis antigens was measured prior to and 4 to 8 weeks after the booster by different assays: enzyme-linked immunosorbent assays (ELISAs) of IgG and IgA against pertussis toxin (PT) and filamentous hemagglutinin (FHA), IgG against pertactin (PRN), opsonophagocytic activity (OPA), and IgG binding to live B. pertussis. There was a significant increase in the IgG activity against PT, FHA, and PRN following the booster immunization (P < 0.001). The prebooster sera showed a geometric mean OPA titer of 65.1 and IgG binding to live bacteria at a geometric mean concentration of 164.9 arbitrary units (AU)/ml. Following the fifth dose, the OPA increased to a titer of 360.4, and the IgG concentration against live bacteria increased to 833.4 AU/ml (P < 0.001 for both). The correlation analyses between the different assays suggest that antibodies against FHA and PRN contribute the most to the OPA and IgG binding.

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Section: Discussionmentioning

confidence: 62%

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Aase¹,

Herstad²,

Merino³

et al. 2011

Clin. Vaccine Immunol.

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“…We have only observed efficient phagocytosis of B. pertussis by (29), by mutagenesis (32), or by neutralizing antibodies (22,31). In contrast, one study reported efficient phagocytosis of antibodyopsonized B. pertussis (26). In this study, phagocytosis was monitored by a flow cytometry assay that involved incubation at 4°C to allow bacterial attachment followed by a shift to 37°C to permit phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

“…A common technique to uncouple the process of attachment from internalization involves incubating the cells at low temperatures, which prevents internalization but not attachment, followed by a shift to 37°C, which restores conditions permissive for internalization. In one study (26), efficient phagocytosis of B. pertussis was reported for an assay that uncoupled attachment and phagocytosis by using a temperature shift. However, the expression of CR3 (Fig.…”

Section: Cr3 Receptor Expressionmentioning

confidence: 99%

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Influence of CR3 (CD11b/CD18) Expression on Phagocytosis ofBordetella pertussisby Human Neutrophils

Mobberley‐Schuman

Weiss

2005

Infect Immun

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CR3 (CD11b/CD18) is expressed on neutrophils, and the engagement of CR3 can promote phagocytosis. CR3 serves as the receptor for the Bordetella pertussis adhesin filamentous hemagglutinin (FHA) and for the adenylate cyclase toxin (ACT), which blocks neutrophil function. The influence of CR3, FHA, and ACT on the phagocytosis of B. pertussis by human neutrophils was examined. The surface expression and function of CR3 are regulated. Tumor necrosis factor alpha (TNF-␣) and gamma interferon (IFN-␥) increased CR3 surface expression, but only TNF-␣ increased the ability of neutrophils to phagocytose B. pertussis, suggesting that elevated CR3 expression alone is not sufficient to promote phagocytosis. Purified FHA and pertussis toxin also increased the surface expression of CR3 on neutrophils, while ACT and the B subunit of pertussis toxin did not affect CR3 expression. FHA-mediated attachment to CR3 can lead to phagocytosis, especially in the absence of ACT. FHA mutants failed to attach and were not phagocytosed by neutrophils. Similarly, an antibody to CR3 blocked both attachment and phagocytosis. The addition of exogenous FHA enhanced the attachment and phagocytosis of wild-type B. pertussis and FHA mutants. Mutants lacking the SphB1 protease, which cleaves FHA and allows the release of FHA from the bacterial surface, were phagocytosed more efficiently than wild-type bacteria. ACT mutants were efficiently phagocytosed, but wild-type B. pertussis or ACT mutants plus exogenous ACT resisted phagocytosis. These studies suggest that the activation and surface expression of CR3, FHA expression, and the efficiency of ACT internalization all influence whether B. pertussis will be phagocytosed and ultimately killed by neutrophils.

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“…Immunoglobulin G (IgG) fractions from pooled sera of pertussis patients with high anti-B. pertussis antibody titers, as measured by enzyme-linked immunosorbent assay (27), were isolated as previously described (32). Polyclonal rabbit anti-B.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Trafficking ofBordetella pertussisin Human Macrophages

et al. 2010

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Although Bordetella pertussis has been observed to survive inside macrophages, its ability to resist or evade degradation in phagolysosomes has not been defined. We here investigated the trafficking of B. pertussis upon entry into human macrophages. During the first hours following phagocytosis, a high percentage of bacteria were destroyed within acidic compartments positive for the lysosome-associated membrane proteins (LAMP). However, roughly one-fourth of the bacteria taken up evade this initial killing event, remaining in nonacidic compartments. Forty-eight hours after infection, the number of intracellular bacteria per cell increased, suggesting that B. pertussis is capable of replicating in this type of compartment. Viable bacteria accumulated within phagosomal compartments positive for the early endosomal marker Rab5 but not the late endosomal marker LAMP. Moreover, B. pertussis-containing phagosomes acquired exogenously added transferrin, indicating that intracellular bacteria have access to extracellular components and essential nutrients via the host cell recycling pathway. Overall, these results suggest that B. pertussis survives and eventually replicates in compartments with characteristics of early endosomes, potentially contributing to its extraordinary ability to persist within hosts and populations.

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Fc Receptor-Mediated Immunity Against Bordetella pertussis

Cited by 60 publications

References 52 publications

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Influence of CR3 (CD11b/CD18) Expression on Phagocytosis ofBordetella pertussisby Human Neutrophils

Intracellular Trafficking ofBordetella pertussisin Human Macrophages

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