Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats

Messora, Michel Reis; Pereira, Luciano José; Foureaux, Renata de Carvalho; Oliveira, Luana Farias de; Sordi, Caio G.; Alves, Ana J.N.; Napimoga, Marcelo Henrique; Nagata, Maria José Hitomi; Ervolino, Edílson; Furlaneto, Flávia Aparecida Chaves

doi:10.1016/j.archoralbio.2016.02.014

Cited by 60 publications

(69 citation statements)

References 38 publications

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“…The right mandibular first molars of the animals of these groups were subjected to SRP with Mini Five 1–2 curettes (Hu-Friedy Co. Inc., Chicago, IL, USA) through ten distal-mesial traction movements on both buccal and lingual surfaces. The furcation and interproximal areas were scaled with the same curettes through cervico-occlusal traction movements [19]. …”

Section: Methodsmentioning

confidence: 99%

“…Using an appropriated software (Data Viewer ® , version 1.5.0, Bruker, Kontich, Belgium), the generated 3 dimensional models were rotated into a standard position as the following criteria: (1) in transaxial plane, the mandibular first molar (M1) had its axis vertically positioned, (2) in coronal plane, the mandibular bone was vertically orientated, with the mesial root of the M1 in the upper position of the image and (3) in sagittal plane, the occlusal surface of M1 was horizontally positioned [19]. Linear measurements on alveolar bone level (ABL) were performed at four different sites: buccal, lingual, interproximal and furcation [19].…”

Section: Methodsmentioning

confidence: 99%

“…Linear measurements on alveolar bone level (ABL) were performed at four different sites: buccal, lingual, interproximal and furcation [19]. For buccal, lingual and interproximal sites, the linear distances from cementoenamel junction (CEJ) to alveolar bone crest (ABC) were measured [20].…”

Section: Methodsmentioning

confidence: 99%

“…For buccal, lingual and interproximal sites, the linear distances from cementoenamel junction (CEJ) to alveolar bone crest (ABC) were measured [20]. For the interproximal site, coronal dataset was analyzed using appropriated software (CT-Analyser ® , version 1.13.5.1+, Bruker, Kontich, Belgium) [19]. For the furcation site, ABL was assessed by measuring the distance between the roof of the furcation and the ABC in the furcation area.…”

Section: Methodsmentioning

confidence: 99%

“…For the furcation site, ABL was assessed by measuring the distance between the roof of the furcation and the ABC in the furcation area. The four linear measurements obtained from each animal were summed to express the ABL value [19]. …”

Section: Methodsmentioning

confidence: 99%

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Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Ricoldi¹,

Furlaneto²,

Oliveira³

et al. 2017

PLoS ONE

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Lactobacillus probiotics have been investigated in periodontitis. However, the effects of the genus Bifidobacterium on periodontitis are hardly known. This study evaluated the effects of the probiotic (PROB) Bifidobacterium animalis subsp. lactis (B. lactis) HN019 as an adjunct to scaling and root planing (SRP) in rats with experimental periodontitis (EP). At baseline, 32 rats were assigned to 4 groups: C (control), PROB, EP-SRP and EP-SRP-PROB. In groups EP-SRP and EP-SRP-PROB, the mandibular first molars of the animals received a ligature. At day 14, the ligatures were removed and SRP was performed. Animals of groups PROB and EP-SRP-PROB were orally administered with 10 mL/day of 109 colony forming units of B. lactis HN019 for 15 days, starting at day 14. Animals were euthanized at day 29. Histomorphometric, microtomographic and immunohistochemical analyses were performed. Microbiological effects of B. lactis on biofilm were also evaluated. Data were statistically analyzed (ANOVA, Tukey; Kruskal-Wallis, Dunn’s; Two-tailed t-test; p<0.05). Group EP-SRP-PROB presented reduced alveolar bone resorption and attachment loss when compared with Group EP-SRP (p<0.05). Group EP-SRP-PROB showed significantly fewer osteoclasts, increased expression of anti-inflammatory cytokines and reduced expression of proinflammatory cytokines compared with Group EP-SRP (p<0.05). B. lactis promoted a higher ratio between aerobic and anaerobic bacteria in biofilm samples (p<0.05). B. lactis HN019 may have a role in the treatment of EP in rats, as an adjunct to SRP.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Ricoldi¹,

Furlaneto²,

Oliveira³

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

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Chronological analysis of periodontal bone loss in experimental periodontitis in mice

Hernández Martínez,

Felix Silva,

Salvador

et al. 2023

Clinical & Exp Dental Res

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ObjectivesPeriodontal disease is understood to be a result of dysbiotic interactions between the host and the biofilm, causing a unique reaction for each individual, which in turn characterizes their susceptibility. The objective of this study was to chronologically evaluate periodontal tissue destruction induced by systemic bacterial challenge in known susceptible (BALB/c) and resistant (C57BL/6) mouse lineages.Material and MethodsAnimals, 6–8 weeks old, were allocated into three experimental groups: Negative control (C), Gavage with sterile carboxymethyl cellulose 2%—without bacteria (Sham), and Gavage with carboxymethyl cellulose 2% + Porphyromonas gingivalis (Pg‐W83). Before infection, all animals received antibiotic treatment (sulfamethoxazole/trimethoprim, 400/80 mg/5 mL) for 7 days, followed by 3 days of rest. Microbial challenge was performed 3 times per week for 1, 2, or 3 weeks. After that, the animals were kept until the completion of 42 days of experiments, when they were euthanized. The alveolar bone microarchitecture was assessed by computed microtomography.ResultsBoth C57BL/6 and BALB/c mice exhibited significant bone volume loss and lower trabecular thickness as well as greater bone porosity compared to the (C) and (Sham) groups after 1 week of microbial challenge (p < .001). When comparing only the gavage groups regarding disease implantation, time and lineage, it was possible to observe that within 1 week of induction the disease was more established in BALB/c than in C57BL/6 (p < .05).ConclusionsOur results reflected that after 1 week of microbial challenge, there was evidence of alveolar bone loss for both lineages, with the loss observed in BALB/c mice being more pronounced.

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The Effects of Omega‐3 Supplementation on Serum Oxidative Stress Parameters in Experimental Periodontitis in an Animal Model

Doğan

2021

Euro J Lipid Sci & Tech

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Experimental studies suggest that omega‐3 may be beneficial at preventing alveolar bone loss (ABL) by modulating host immune response. The aim of this study is to evaluate the effects of omega‐3 supplementation on ABL and serum oxidative biomarkers in a ligature‐induced periodontitis model. Twenty‐four Wistar albino rats are divided into 3 groups: control (C, n = 8); ligature‐induced periodontitis (L, n = 8); and omega‐3 plus ligature‐induced periodontitis (O+L, n = 8). All rats are fed with ad libitum diet, and O+L group is additionally supplemented with omega‐3 fish oil by oral gavage for 44 days. Experimental periodontitis is induced by placement of ligatures around the maxillary second molars of rats in L and O+L groups. ABL is measured histomorphometrically and serum 8‐hydroxy‐2'‐deoxyguanosine, total antioxidant capacity, and total oxidant status (TOS) are analyzed. ABL is higher in L and O+L groups than the C group. Omega‐3 supplementation is significantly reduced ABL in group O+L, compared to L group. Furthermore, TOS levels are lower in O+L group than L group. It is suggested that omega‐3 administration may reduce ABL and serum TOS levels, which supports the antioxidant role of omega‐3 on periodontal disease pathogenesis. Practical applications: Adjunctive use of omega‐3 supplementation in periodontitis seems beneficial, although biochemical mechanisms underlying the conflicting results have not been completely understood. On the other hand, oxidative stress has been used as an appropriate target for host modulation therapies in periodontal disease. Omega‐3 supplementation in ligature‐induced periodontitis in an animal model successfully reduces alveolar bone loss by modulating serum total oxidant status, which may provide a novel pathway in periodontal disease pathogenesis.

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Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats

Abstract: Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.

Cited by 60 publications

References 38 publications

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Chronological analysis of periodontal bone loss in experimental periodontitis in mice

The Effects of Omega‐3 Supplementation on Serum Oxidative Stress Parameters in Experimental Periodontitis in an Animal Model

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