In the recent decades, peoples concentrate more on reduced-consumption of diets containing saturated fatty acids and replace them with essential polyunsaturated ones, including omega-3 and omega-6 fatty acids, due to their promising nutritional benefits. Therefore, the production of chicken meat having lower fat compositions, and riches in pro-healthy fatty acid and other macro-and micronutrients deserves great attention in the food industry. The research aimed to characterize meat oils of two commercially available, domestic and poultry, chickens in the Northern part of Bangladesh (Rajshahi), followed by the investigation of their nutritional compositions and hypoglycemic effect in vivo. In this work, the oil was isolated from the dried meat sources by Soxhlet extractor and purified using a rotary evaporator. We subsequently characterized meat oil in terms of various parameters, such as iodine value, saponification number, unsaponifiable matter, free fatty acid (FFA) contents, peroxide value, and acid value. Besides, the nutritional parameters of chicken oil were investigated as percentages of essential fatty acids and several other micro-and macronutrients using gas-liquid chromatography and other conventional methods. Finally, Alloxan-induced Wister rats were fed with oil and chicken flesh to investigate the hypoglycemic effects. The experimental analyses show that chicken oil possesses a significant proportion of omega-3 and omega-6 fatty acid, macro-and micronutrients. Besides, oil and flesh were found to lower blood glucose levels in diabetic rats despite flesh could not significantly show hypoglycemic properties.