Fat Chance: The Rejuvenation of Irradiated Skin

Borrelli, Mimi R.; Patel, Ronak A.; Sokol, Jan; Nguyen, Dung; Momeni, Arash; Longaker, Michael T.; Wan, Derrick C.

doi:10.1097/gox.0000000000002092

Cited by 31 publications

(13 citation statements)

References 86 publications

(102 reference statements)

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“…The mechanisms by which ASCs promote fat graft vascularization are thought to be largely mediated via paracrine signaling. 4 Here, we…”

Section: Discussionmentioning

confidence: 95%

“…2,3 The adipose-derived stromal cells (ASCs) within the stromal vascular fraction (SVF) of fat are thought to drive these regenerative effects. 4 Evidence in support for this hypothesis has stemmed from several clinical and preclinical studies that demonstrate, compared to fat alone, grafts enriched with ASCs, also known as cellassisted lipotransfer, undergo less resorption and have improved vascularization and histological structure. [5][6][7] Recent work has indicated that ASCs are a heterogeneous population comprised of distinct subpopulations of cells with differing functional capacities; bone morphogenetic protein receptor (BMPR)-1A…”

Section: Introductionmentioning

confidence: 99%

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CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat

Borrelli

Patel

Blackshear

et al. 2020

Stem Cells Translational Medicine

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Fat grafting is a surgical technique able to reconstruct and regenerate soft tissue. The adipose-derived stromal cells (ASCs) within the stromal vascular fraction are believed to drive these beneficial effects. ASCs are increasingly recognized to be a heterogeneous group, comprised of multiple stem and progenitor subpopulations with distinct functions. We hypothesized the existence of an ASC subpopulation with enhanced angiogenic potential. Human ASCs that were CD34+CD146+, CD34+CD146−, or CD34+ unfractionated (UF) were isolated by flow cytometry for comparison of expression of proangiogenic factors and endothelial tube-forming potential. Next, lipoaspirate was enriched with either CD34+CD146+, CD34+CD146−, CD34+ UF ASCs, or was not enriched, and grafted beneath the scalp skin of immunodeficient CD-1 Nude mice (10 000 cells/200 μL/graft). Fat retention was monitored radiographically more than 8 weeks and fat grafts were harvested for histological assessment of quality and vascularization. The CD34+CD146+ subpopulation comprised 30% of ASCs, and exhibited increased expression of vascular endothelial growth factor and angiopoietin-1 compared to CD34+CD146− and CD34+ UF ASCs, and increased expression of fibroblast growth factor-2 compared to CD34+CD146− ASCs. The CD34+CD146+ subpopulation exhibited enhanced induction of tube-formation compared to CD34+CD146− ASCs. Upon transplantation, fat enriched CD34 +CD146+ ASCs underwent less resorption and had improved histologic quality and vascularization. We have identified a subpopulation of CD34+ ASCs with enhanced angiogenic effects in vitro and in vivo, likely mediated by increased expression of potent proangiogenic factors. These findings suggest that enriching lipoaspirate with CD34+CD146+ ASCs may enhance fat graft vascularization and retention in the clinical setting.

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“…The mechanisms by which ASCs promote fat graft vascularization are thought to be largely mediated via paracrine signaling. 4 Here, we…”

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat

Borrelli

Patel

Blackshear

et al. 2020

Stem Cells Translational Medicine

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“…Autologous fat grafting has gained acceptance to improve skin texture and postinflammatory hyperpigmentation associated with contour deformities of face (M. M. Bashir et al, 2019). Fat, being an autologous tissue, seems to be an ideal choice as filling material and is preferred owing to its abundant availability in areas like abdomen, buttocks, thighs, and arms without any significant donor site morbidity (Borrelli et al, 2019). Fat injection sessions can be repeated for further volume enhancement and fine‐tuning if needed (M. M. Bashir et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Fat grafts enriched with ex‐vivo expanded adipose stem cells improve hyperpigmentation of face

Bashir

Afzaal

et al. 2023

Cell Biology International

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Facial contour deformities associated with pigmentary changes are of major concern for plastic surgeons being difficult to treat. No definite ideal treatment option is available to address simultaneously both the contour defects as well as related hyperpigmentation. The aim of the current study is to compare long-term effects of conventional adipose tissue grafting and ex-vivo expanded ASC-enriched adipose tissue grafting for the treatment of contour deformities related pigmentary changes on the face. In this study, 80 patients of contour deformities of face with hyperpigmentation were recruited after informed consent. Two techniques, that is, conventional fat grafting (C-FG) and fat grafts enriched with expanded adipose stem cells (FG-ASCs) were used to address the pigmentation. Both techniques were explained to patients and enrolled patients were divided into two groups, that is, C-FG and FG-ASCs as per patients' choice and satisfaction. Patients of the FG-ASCs group were treated with FG-ASCs while patients of C-FGs group were treated with C-FG (without expanded ASCs). Patients were followed for 12 months and improvement in face pigmentation was assessed clinically as well as measured objectively. Patients' satisfaction was also documented as highly satisfied, satisfied, and unsatisfied. This clinical trial was registered at www.clinicaltrials.gov with ID: NTC03564808. Mean age of patients was 24.42 (±4.49), 66 patients were females.Forehead was involved in 61.20% cases, cheek in 21.20% cases, chin in 11.20% cases, and nose in 6.20% cases. In GF-ASCs group, the integrated color density was decreased (1.08 × 10 6 ± 4.64 × 10 5 ) as compared with C-FG group (2.80 × 10 5 ± 1.69 × 10 5 ). Patients treated with fat grafts enriched with expanded ASCs were significantly more satisfied as compared with patients treated with C-FG only. In conclusion, ASC-enriched autologous fat grafting is preferred option for improving the contour deformities related increased pigmentation of face skin.

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“…Some of these proposed therapeutics include antioxidants like tocopherol targeting transforming growth factor beta, autologous fat transfer targeting fibroblast activity, and adipose‐derived stem cells which work through the release of growth factors and cytokines contributing to the mitigation of radiation‐damaged skin. 7 , 8 , 9 Although these therapies show clinical promise, to date, no single therapeutic option has been consistently effective in the management of CRF. 2 …”

Section: Introductionmentioning

confidence: 99%

Laser therapy as a treatment for chronic radiation fibrosis

et al. 2022

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Background Chronic radiation fibrosis (CRF) is a long‐term sequala of radiation therapy that has a significant impact on patient quality of life. There is no standard of care or single therapeutic modality that has been found to be consistently effective. Objective To describe our experience using fractional 10,600 nm carbon dioxide (CO2) laser therapy and vascular laser therapy in a series of patients with CRF. Methods Patients presenting to the dermatology service for CRF were evaluated for laser therapy eligibility. Patients were eligible if they had a clinical diagnosis of CRF confirmed by physical examination. Results We identified five patients with CRF treated with fractional ablative CO2 laser and vascular laser. Patients were a median age of 57 years old, and the amount of time between the initiation of radiotherapy and laser treatment ranged between 3 months and 40 years. The satisfactory response was achieved in all cases. Limitations Lack of standardized laser protocol, small sample size, lack of a control group, different anatomical locations Conclusion Fractional ablative and vascular laser therapy may serve as an additional treatment for CRF, leading to functional improvements.

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Fat Chance: The Rejuvenation of Irradiated Skin

Cited by 31 publications

References 86 publications

CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat

CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat

Fat grafts enriched with ex‐vivo expanded adipose stem cells improve hyperpigmentation of face

Laser therapy as a treatment for chronic radiation fibrosis

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