Fasting-induced torpor in Mus musculus and its implications in the use of murine models for human obesity studies

Behavioral thermoregulation in mice inoculated with influenza virus

“…Since infected mice decrease their food intake, we compared the Tb of mice fed ad lib with that of food-deprived mice. We found that 48 h of starvation significantly lowered Tb of healthy mice, and this is consistent with results of other studies showing that food deprivation can lead to a decrease in Tb [e.g., (6,7,18)]. However, the fall in Tb of mice which were food-deprived for 48 h was less than the fall in Tb of infected mice after 2 days of very low food intake.…”

Behavioral thermoregulation in mice inoculated with influenza virus

“…Since infected mice decrease their food intake, we compared the Tb of mice fed ad lib with that of food-deprived mice. We found that 48 h of starvation significantly lowered Tb of healthy mice, and this is consistent with results of other studies showing that food deprivation can lead to a decrease in Tb [e.g., (6,7,18)]. However, the fall in Tb of mice which were food-deprived for 48 h was less than the fall in Tb of infected mice after 2 days of very low food intake.…”

Leptin Raises Defended Body Temperature without Activating Thermogenesis

“…The effect of leptin during the dark phase was much more pronounced. In agreement with earlier data (Himms-Hagen, 1985;Webb et al, 1982), the pair-fed mice, in the relative cold of 21 C and in the absence of sufficient food, went into torpor at the end of the night: the Tb dropped conspicuously (Figure S1E). The much-lowered Tb resulted necessarily in a diminished metabolic rate (Figure S1F).…”

MitoRACE: evaluating mitochondrial function in vivo and in single cells with subcellular resolution using multiphoton NADH autofluorescence

“…Mitochondrial NADH flux measured by mitoRACE showed acute responsiveness to both physiological and pharmacological modulation, and the magnitude of changes in flux consequent to these perturbations was similar to values previously reported by in vivo studies evaluating changes in oxygen consumption associated with muscle contraction (Hood et al 1986;Marcinek et al 2003), dietary fasting (Webb et al 1982;Marvyn et al 2016) and mitochondrial uncoupling (Marcinek et al 2003). In agreement with previous studies evaluating whole muscle oxygen uptake in skeletal muscle (Hood et al 1986;Marcinek et al 2003), we found that physiological modulation of metabolism by muscle contraction was associated with a significantly larger increase in mitochondrial flux (ß7-fold) (Hood et al 1986;Marcinek et al 2003) compared to pharmacological modulation by uncoupling (ß3 fold) (Marcinek et al 2003), reflecting the different metabolic mechanisms involved in these states.…”

Behavioral thermoregulation in mice inoculated with influenza virus

“…Since infected mice decrease their food intake, we compared the Tb of mice fed ad lib with that of food-deprived mice. We found that 48 h of starvation significantly lowered Tb of healthy mice, and this is consistent with results of other studies showing that food deprivation can lead to a decrease in Tb [e.g., (6,7,18)]. However, the fall in Tb of mice which were food-deprived for 48 h was less than the fall in Tb of infected mice after 2 days of very low food intake.…”

Leptin Raises Defended Body Temperature without Activating Thermogenesis

“…The effect of leptin during the dark phase was much more pronounced. In agreement with earlier data (Himms-Hagen, 1985;Webb et al, 1982), the pair-fed mice, in the relative cold of 21 C and in the absence of sufficient food, went into torpor at the end of the night: the Tb dropped conspicuously (Figure S1E). The much-lowered Tb resulted necessarily in a diminished metabolic rate (Figure S1F).…”

MitoRACE: evaluating mitochondrial function in vivo and in single cells with subcellular resolution using multiphoton NADH autofluorescence

“…Mitochondrial NADH flux measured by mitoRACE showed acute responsiveness to both physiological and pharmacological modulation, and the magnitude of changes in flux consequent to these perturbations was similar to values previously reported by in vivo studies evaluating changes in oxygen consumption associated with muscle contraction (Hood et al 1986;Marcinek et al 2003), dietary fasting (Webb et al 1982;Marvyn et al 2016) and mitochondrial uncoupling (Marcinek et al 2003). In agreement with previous studies evaluating whole muscle oxygen uptake in skeletal muscle (Hood et al 1986;Marcinek et al 2003), we found that physiological modulation of metabolism by muscle contraction was associated with a significantly larger increase in mitochondrial flux (ß7-fold) (Hood et al 1986;Marcinek et al 2003) compared to pharmacological modulation by uncoupling (ß3 fold) (Marcinek et al 2003), reflecting the different metabolic mechanisms involved in these states.…”

Behavioral thermoregulation in mice inoculated with influenza virus

“…Since infected mice decrease their food intake, we compared the Tb of mice fed ad lib with that of food-deprived mice. We found that 48 h of starvation significantly lowered Tb of healthy mice, and this is consistent with results of other studies showing that food deprivation can lead to a decrease in Tb [e.g., (6,7,18)]. However, the fall in Tb of mice which were food-deprived for 48 h was less than the fall in Tb of infected mice after 2 days of very low food intake.…”

Leptin Raises Defended Body Temperature without Activating Thermogenesis

“…The effect of leptin during the dark phase was much more pronounced. In agreement with earlier data (Himms-Hagen, 1985;Webb et al, 1982), the pair-fed mice, in the relative cold of 21 C and in the absence of sufficient food, went into torpor at the end of the night: the Tb dropped conspicuously (Figure S1E). The much-lowered Tb resulted necessarily in a diminished metabolic rate (Figure S1F).…”

MitoRACE: evaluating mitochondrial function in vivo and in single cells with subcellular resolution using multiphoton NADH autofluorescence

“…Mitochondrial NADH flux measured by mitoRACE showed acute responsiveness to both physiological and pharmacological modulation, and the magnitude of changes in flux consequent to these perturbations was similar to values previously reported by in vivo studies evaluating changes in oxygen consumption associated with muscle contraction (Hood et al 1986;Marcinek et al 2003), dietary fasting (Webb et al 1982;Marvyn et al 2016) and mitochondrial uncoupling (Marcinek et al 2003). In agreement with previous studies evaluating whole muscle oxygen uptake in skeletal muscle (Hood et al 1986;Marcinek et al 2003), we found that physiological modulation of metabolism by muscle contraction was associated with a significantly larger increase in mitochondrial flux (ß7-fold) (Hood et al 1986;Marcinek et al 2003) compared to pharmacological modulation by uncoupling (ß3 fold) (Marcinek et al 2003), reflecting the different metabolic mechanisms involved in these states.…”