Fast monitoring of tooth erosion caused by medicaments used in the treatment of respiratory diseases by ATR-FTIR and μ-EDXRF analysis

Abstract

The present study aimed to evaluate the erosive potential of four most commonly prescribed syrup medicaments for respiratory diseases. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy combined with multivariate statistical analysis and micro-energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping was performed. Fifty-five root dentin fragments obtained from the buccal surface of 30 bovine teeth were prepared and divided into five experimental groups (n = 10): control-artificial saliva (S), acebrofilin hydrochloride (AC), ambroxol hydrochloride (AM), bromhexine hydrochloride (BR), and salbutamol sulfate (SS). The S group was stored only in artificial saliva and the other groups were treated with the medicaments (immersed for 1 min in 3 mL of the medication, three times daily, with 1-h intervals between the immersion cycles, during 5 days, 15 immersion cycles). There were a significant decrease in the Ca and P weight percentages (wt%) for dentin after medication treatments, except for AC (p > 0.05). Mineral content of dentin showed a clear gradation with increasing Ca and P wt% reduction in the order S < AC < AM < BR < SS. SS resulted in a significant increase in Ca/P ratio when compared to the control (p < 0.001). ATR-FTIR combined with multivariate, statistical analysis can quickly and reliably indicate extent of dentin erosion. Considering syrups with high-erosive potential should always follow with proper oral hygiene practices or search for an alternative medications void of such detrimental effects. Regular and prolonged use of these medicaments might bear the risk of causing erosion.

Chemical and morphological evaluation of enamel and dentin near cavities restored with conventional and zirconia modified glass ionomer subjected to erosion‐abrasion

“…In this study, SEM was selected to analyze the effect of the abrasion, erosion, and saliva on enamel and dentin morphology to measure substance loss accurately and provide visual information on surface precipitates and change of the surface morphology. Conversely, μ‐EDXRF provides a specific method to determine the concentration of chemical elements on substratum surfaces, being previously used in some studies in dentistry (De Carvalho Filho et al, ; Gomes et al, , ; Soares et al, ; Soares & De Carvalho Filho, ; Soares, De Oliveira, et al, ; Soares et al, ; Soares et al, ).…”

“…We declare that all researchers directly involved in animal experimentation followed the Ethical Principles in Animal Experimentation; Informed Consent: The research does not involve human participants and there is not informed consent). A total of 25 freshly extracted bovine anterior teeth were selected based on their dimensions, similar morphology, and absence of any cracks, staining, or defects (Gomes et al, ). Subsequently, the teeth were cleaned and stored in a 0.1% aqueous thymol solution (Soares et al, ).…”

“…The root dentin was sectioned again in the middle, resulting in two blocks per tooth ( n = 50, approximately 8 mm long, 8 mm wide, and 6 mm thick; Nahorny et al, ). In sequence, the tooth crowns were prepared and sectioned to obtain two enamel blocks per tooth ( n = 50, approximately 8 mm long, 8 mm wide, and 4 mm thick) (Gomes et al, ). All 100 slabs were ground and polished to produce parallel surfaces (labial and lingual sites) with wet 600 and 1,500‐grit silicon carbide paper (Arotec, Cotia, SP, Brazil) at 150 rpm, and subsequently subjected to ultrasonication in deionized water for 10 min (Maxiclean 1,450, Merse, Campinas, SP, Brazil) to remove residual particles.…”

“…Surface area mapping ( n = 100) was performed on each dentin and enamel specimen to evaluate the Ca and P weight percentages (wt%). The parameters used were as follows: 40 × 30 points; 20 μm steps, 5 s per point count rate; 15 kV voltage in the tube (Gomes et al, ). μ‐EDXRF analysis was performed with points that were 50 μm in diameter at a penetration depth of only a few microns (de Sá Ferreira et al, ).…”

Adverse effects of respiratory disease medicaments and tooth brushing on teeth: A scanning electron microscopy, X‐ray fluorescence and infrared spectroscopy study

“…Dental erosion is the focus of many researchers and clinicians due to high prevalence and multiple etiologies. According to biotribology, is better defined as dental corrosion, characterized by the loss of dental structure as a result of demineralization by acid substances from intrinsic or extrinsic factors (Baumann, Kozik, Lussi, & Carvalho, ; Fita & Kaczmarek, ; Gomes, Bhattacharjee, Carvalho, & Soares, ; Kanzow, Wegehaupt, Attin, & Wiegand, ; Lussi & Carvalho, ; Nahorny et al, ; Passos, Melo, Vasconcellos, Rodrigues, & Santiago, ; Poggio, Gulino, Mirando, Colombo, & Pietrocola, ; Scatena, Galafassi, Gomes‐Silva, Borsatto, & Serra, ; Soares & De Carvalho Filho, ; Thomas, Pai, & Amit, ; Yönel et al, ). Use of acidic medicaments is one such extrinsic factors of erosion.…”