Fast degrading elastomer stented fascia remodels into tough and vascularized construct for tracheal regeneration

Wu, Wei; Jia, Sansan; Chen, Wan-Li; Liu, Xuzheng; Zhang, Siqian

doi:10.1016/j.msec.2019.02.108

Cited by 14 publications

(13 citation statements)

References 38 publications

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“…Similar mechanical behaviour, even though for a different scaffold architecture, has been recorded for three-layer systems made of porous PGS tubes wrapped with PCL electrospun fibres and reinforced by PCL rings (tensile load at break of around 5.5 N), where PGS significantly contributed to the elastomeric response [28]. The use of electrospun sheets to increase tensile strength in composites has been described in the literature for microfibre-based netting materials [29][30][31].…”

Section: Characterisation Of the Tensile Response Of The Pcl-pgs-bgs supporting

confidence: 65%

Multi-layer Scaffolds of Poly(caprolactone), Poly(glycerol sebacate) and Bioactive Glasses Manufactured by Combined 3D Printing and Electrospinning

2020

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Three-dimensional (3D) printing has been combined with electrospinning to manufacture multi-layered polymer/glass scaffolds that possess multi-scale porosity, are mechanically robust, release bioactive compounds, degrade at a controlled rate and are biocompatible. Fibrous mats of poly (caprolactone) (PCL) and poly (glycerol sebacate) (PGS) have been directly electrospun on one side of 3D-printed grids of PCL-PGS blends containing bioactive glasses (BGs). The excellent adhesion between layers has resulted in composite scaffolds with a Young’s modulus of 240–310 MPa, higher than that of 3D-printed grids (125–280 MPa, without the electrospun layer). The scaffolds degraded in vitro by releasing PGS and BGs, reaching a weight loss of ~14% after 56 days of incubation. Although the hydrolysis of PGS resulted in the acidification of the buffer medium (to a pH of 5.3–5.4), the release of alkaline ions from the BGs balanced that out and brought the pH back to 6.0. Cytotoxicity tests performed on fibroblasts showed that the PCL-PGS-BGs constructs were biocompatible, with cell viability of above 125% at day 2. This study demonstrates the fabrication of systems with engineered properties by the synergy of diverse technologies and materials (organic and inorganic) for potential applications in tendon and ligament tissue engineering.

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Section: Characterisation Of the Tensile Response Of The Pcl-pgs-bgs supporting

confidence: 65%

Multi-layer Scaffolds of Poly(caprolactone), Poly(glycerol sebacate) and Bioactive Glasses Manufactured by Combined 3D Printing and Electrospinning

2020

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“…Sixteen studies were isolated pertaining to the application of electrospun nanofibers as tissue engineered tracheal grafts (TETG) for reconstruction of long segment tracheal defects. 1,[4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] Although the specifics of scaffold design and fabrication vary for each study, Clarke and colleagues describe a representative process: First a custom mandrel was created from stainless steel with dimensions matching those obtained from native juvenile sheep tracheas. 16 Once the desired polymer blend was selected, this solution was electrospun using a custom designed apparatus comprised of 20 gauge blunt tip needles and a high voltage direct current (DC) power supply (Figure 3).…”

Section: Tracheal Reconstructionmentioning

confidence: 99%

“…8,14 Electrospun polycaprolactone (PCL) scaffolds have also been explored in TETGs. 6,12,15,17,18 Townsend et al studied TETGs derived from PCL in an ovine model and showed that these grafts were suturable, airtight and durable. 12 However, an overgrowth of fibrous tissue around the site of reconstruction was noted.…”

Section: Sheepmentioning

confidence: 99%

Applications of Electrospinning for Tissue Engineering in Otolaryngology

Heilingoetter

Smith

Malhotra

et al. 2020

Ann Otol Rhinol Laryngol

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Objective: In tissue engineering, biomaterials create a 3D scaffold for cell-to-cell adhesion, proliferation and tissue formation. Because of their similarity to extracellular matrix and architectural adaptability, nanofibers are of particular interest in tissue engineering. Electrospinning is a well-documented technique for nanofiber production for tissue engineering scaffolds. Here we present literature on the applications of electrospinning in the field of otolaryngology. Review Methods: A PubMed database search was performed to isolate articles published about applications of electrospun nanofibers for tissue engineering in otolaryngology. Study design, size, material tested, site of application within the head and neck, and outcomes were obtained for each study. Results: Almost all data on electrospinning in otolaryngology was published in the last 6 years (84%), highlighting its novelty. A total of 25 pre-clinical studies were identified: 9 in vitro studies, 5 in vivo animal studies, and 11 combination studies. Sites of application included: tracheal reconstruction (n = 16), tympanic membrane repair (n = 3), cranial nerve regeneration (n = 3), mastoid osteogenesis (n = 1) and ear/nose chondrogenesis (n = 2). Implications for Practice: Tissue engineering is a burgeoning field, with recent innovative applications in the field of otolaryngology. Electrospun nanofibers specifically have relevant applications in the field of otolaryngology, due in part to their similarity to native extracellular matrix, with emerging areas of interest being tympanic membrane repair, cranial nerve regeneration and tracheal reconstruction.

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“…The silk fibroin tube and the polycaprolactone ring were prefrozen at −80°C for 2 h. Then, they were put into a freeze dryer for vacuum drying, and the polycaprolactone ring was fixed on the silk fibroin tube at a spacing of 3 mm using an electrostatic spinning technique 22 . Finally, a biodegradable biomimetic tracheal scaffold with a composite of biomaterials and synthetic materials was obtained.…”

Section: Methodsmentioning

confidence: 99%

Preparation and evaluation of a silk fibroin–polycaprolactone biodegradable biomimetic tracheal scaffold

Liu

Feng

et al. 2022

J Biomed Mater Res

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In tracheal tissue engineering, the construction of tracheal scaffolds with adequate biodegradable mechanical capacity and biological functions that mimic the structure of a natural trachea is challenging. To explore the feasibility of preparing biomimetic degradable scaffolds with C‐type cartilage rings and an inner tracheal wall of polycaprolactone and silk fibroin. A mold was made according to the diameter of a rabbit trachea, and a silk fibroin tube and polycaprolactone ring attached to the tube were obtained by solution casting. The ring was fixed to the tube at a specific spacing using electrostatic spinning technology to construct a biomimetic tracheal scaffold; its porous structure was observed by scanning electron microscopy, its degradation properties were determined by in vitro enzymatic hydrolysis and its mechanical properties were obtained by pressure testing. The composite scaffold was transplanted subcutaneously into a rabbit model, and the scaffold was taken at 1, 2, and 4 weeks after surgery for sectioning to observe pre‐vascularization. The Medical Ethics Committee of Guangdong Provincial People's Hospital approved the study. The general view of the biomimetic scaffold: the polycaprolactone ring was fixed firmly on the outer wall of the silk fibroin tube; the two corresponded in size, and they fitted closely. The surface of the polycaprolactone ring was smooth and dense, while the surface of the silk fibroin tube could be seen as a uniform porous structure. Scanning electron microscopy showed that the surface and profile of the fibroin tube had a uniform pore size and distribution. The pores were connected to form a network. In vitro, enzymatic hydrolysis experiments confirmed that the fibroin was degraded easily, with most being degraded at the end of week 1. The degradation slowed at 2, 3, and 4 weeks, while the degradation of polycaprolactone was extremely slow. A compression test showed that the compressive resistance of the silk fibroin–polycaprolactone biomimetic scaffolds was much better than that of the rabbit trachea at close thickness. In the tissue staining experiments, as the material degraded, fibrous tissues and blood vessels grew to replace the material, allowing the scaffold to obtain a blood supply and better mechanical properties. A quantitative analysis of CD31 showed that the results for the vascularization of the scaffold were better at 4 weeks than at 2 weeks following subcutaneous grafting (P < .05). The results confirmed that it is feasible to prepare porous, degradable silk fibroin–polycaprolactone biomimetic scaffolds with good mechanical properties and epithelial biological functions by mold casting.

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Fast degrading elastomer stented fascia remodels into tough and vascularized construct for tracheal regeneration

Cited by 14 publications

References 38 publications

Multi-layer Scaffolds of Poly(caprolactone), Poly(glycerol sebacate) and Bioactive Glasses Manufactured by Combined 3D Printing and Electrospinning

Multi-layer Scaffolds of Poly(caprolactone), Poly(glycerol sebacate) and Bioactive Glasses Manufactured by Combined 3D Printing and Electrospinning

Applications of Electrospinning for Tissue Engineering in Otolaryngology

Preparation and evaluation of a silk fibroin–polycaprolactone biodegradable biomimetic tracheal scaffold

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