Fast anisotropic growth of the biomineralized zinc phosphate nanocrystals for a facile and instant construction of laccase@Zn3(PO4)2 hybrid nanoflowers

Facile preparation of amorphous cobalt phosphate as inorganic carrier for direct separation and immobilization of his-tagged β-glucosidase from cell lysate

“…The target enzyme does not need to be extracted in advance, but can be purified by and immobilized directly from the crude enzyme solution onto the support material. This is significantly different from previous reports of similar materials for immobilizing enzymes, 44,45 since those works still use pure enzymes (purchased from biological companies) for immobilization. The additional cost of using pure enzymes limits the large-scale application of immobilized enzyme catalysts in industry.…”

“…Phosphate materials can be prepared by a simple one-step precipitation method and used as inorganic carrier materials for immobilization of enzymes. The stability of the immobilized enzyme is enhanced by biomineralization [24][25][26][27] to obtain enzyme-phosphate hybrid nanoflowers. Although phosphate has been only used for immobilization of pure enzymes, the interaction between metal ions in phosphate and related amino acids of enzymes has been reported.…”

Co-immobilized bienzyme of horseradish peroxidase and glucose oxidase on dopamine-modified cellulose–chitosan composite beads as a high-efficiency biocatalyst for degradation of acridine

“…This is also due to the carrier protection and the altered structure of the protein of the immobilized bienzyme, which reduced the effect of pH factors. 43 …”

“…This is also due to the carrier protection and the altered structure of the protein of the immobilized bienzyme, which reduced the effect of pH factors. 43 3.6 Effect of HBT dosage on degradation of acridine by Ce-Cs@DA/HRP-GOD beads Fig. 6A shows the effect of the HBT dosage on the degradation rate of acridine by the co-immobilized biocatalyst Ce-Cs@DA/ HRP-GOD beads.…”

Trypsin/Zn3(PO4)2 Hybrid Nanoflowers: Controlled Synthesis and Excellent Performance as an Immobilized Enzyme

“…Generally, PINFs can be classified according to the types of the inorganic component and the organic component [ 2 , 6 , 7 ]. A series of inorganic phosphate, such as zinc [ 7 , 8 ], copper [ 9 , 10 ], calcium [ 11 , 12 ], manganese [ 13 , 14 ] and cobalt [ 15 , 16 ] phosphate, have been employed to construct PINFs. Additionally, several organic biological macromolecules, including enzymes [ 17 , 18 ], proteins [ 19 , 20 ] and nucleic acids [ 21 , 22 ], have been reported for the synthesis of PINFs.…”