Fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics

Pujol‐Vila, Ferran; Vigués, Núria; Díaz‐González, María; Muñoz-Berbel, Xavier; Mas, Jordi

doi:10.1016/j.bios.2014.08.030

Cited by 19 publications

(6 citation statements)

References 22 publications

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“…5d). Even the EC 50 , around 3.9 mg L -1 , coincided with that obtained with the reflectomery assay, and was in agreement with those previously reported for optical kinetic analysis of ferricyanide reduction [20] and with the standard method Microtox® [22]. According to this, chromatic analysis of BPDs represents a suitable method for quantitative determination of water toxicity, independently on the transduction method.…”

Section: Bacterial Entrapment Efficiency and Stability On Pdssupporting

confidence: 90%

“…This intriguing fact may be derived from inherent nature of kinetic and single measurement analysis. That is, kinetic analysis relies on a reversible and non-accumulative magnitude, and conversely single point analysis relies on an irreversible and accumulative magnitude [20]. Thus, the present cellulose-based bioassay represents a low-cost, simple, robust and reliable strategy for quick in-situ determination of toxicity with minimal instrumentation and without problems of portability.…”

Section: Bacterial Entrapment Efficiency and Stability On Pdsmentioning

confidence: 99%

“…Considering this fact, our group has recently developed a toxicity bioassay based on the optical determination of the bacterial ferricyanide reduction kinetics [20].…”

Section: Introductionmentioning

confidence: 99%

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Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

Pujol‐Vila

Vigués

Guerrero-Navarro

et al. 2016

Analytica Chimica Acta

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Water quality assessment requires a continuous and strict analysis of samples to guarantee compliance with established standards. Nowadays, the increasing number of pollutants and their synergistic effects lead to the development general toxicity bioassays capable to analyse water pollution as a whole. Current general toxicity methods, e.g. Microtox(®), rely on long operation protocols, the use of complex and expensive instrumentation and sample pre-treatment, which should be transported to the laboratory for analysis. These requirements delay sample analysis and hence, the response to avoid an environmental catastrophe. In an attempt to solve it, a fast (15 min) and low-cost toxicity bioassay based on the chromatic changes associated to bacterial ferricyanide reduction is here presented. E. coli cells (used as model bacteria) were stably trapped on low-cost paper matrices (cellulose-based paper discs, PDs) and remained viable for long times (1 month at -20 °C). Apart from bacterial carrier, paper matrices also acted as a fluidic element, allowing fluid management without the need of external pumps. Bioassay evaluation was performed using copper as model toxic agent. Chromatic changes associated to bacterial ferricyanide reduction were determined by three different transduction methods, i.e. (i) optical reflectometry (as reference method), (ii) image analysis and (iii) visual inspection. In all cases, bioassay results (in terms of half maximal effective concentrations, EC50) were in agreement with already reported data, confirming the good performance of the bioassay. The validation of the bioassay was performed by analysis of real samples from natural sources, which were analysed and compared with a reference method (i.e. Microtox). Obtained results showed agreement for about 70% of toxic samples and 80% of non-toxic samples, which may validate the use of this simple and quick protocol in the determination of general toxicity. The minimum instrumentation requirements and the simplicity of the bioassay open the possibility of in-situ water toxicity assessment with a fast and low-cost protocol.

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Section: Bacterial Entrapment Efficiency and Stability On Pdssupporting

confidence: 90%

Section: Bacterial Entrapment Efficiency and Stability On Pdsmentioning

confidence: 99%

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Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

Pujol‐Vila

Vigués

Guerrero-Navarro

et al. 2016

Analytica Chimica Acta

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“…Being a model organism for laboratories, almost every aspect of E.coli, from its genome to its proteinmaking capability, has been exploited (Lee, 2009). In bacterial metabolism, oxygen acts as the hydrogen acceptor in the TCA cycle under the aerobic condition for growth and respiration (Hadjipetrou, 1966).…”

Section: Introductionmentioning

confidence: 99%

Chromatic Toxicity Bioassay of Water through Bacterial Ferricyanide Reduction

Shamim

Shaikh

Kanwal

et al. 2021

Preprint

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Water quality assessment demands a precise anatomization of specimens that comply with acclaimed water purity standards. Today, the growing number of toxicants and their synergistic consequences make it necessary to develop general toxicity assays able to examine and determine water pollution. Contemporary general toxicity methods hinder specimen analysis due to their prolonged operation protocols. Also, the equipment involved is very expensive that not everyone can afford it. In an effort to resolve these drawbacks, a quick and cost effective toxicity bioassay based on chromatic changes related to bacterial ferricyanide reduction is introduced here. E.coli cells (Model Bacteria) were stably confined on four supports: Cellulose-based Paper Discs, Silica 60, Polystyrene, and Acrylic Beads, which remained useful for a long period at -20ºC. Copper was used as a model toxic agent to perform Bioassay Assessment. Chromatic changes related to bacterial ferricyanide reduction were determined by visual inspection. Cellulose Paper Discs, Polystyrene, and Acrylic beads showed good results and better viability, while Silica 60 proved itself as a weak support and resulted in poor viability.

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“…The starting iron donors for PB formation are a specific concentration mixture of potassium ferricyanide and ferric ammonium citrate. Bacteria readily reduce ferricyanide to ferrocyanide. − However, this reduced form cannot spontaneously react with ferric ammonium citrate at the provided dilution. The bond between iron and citrate cannot be spontaneously broken by the presence of ferrocyanide or bacteria but requires light activation.…”

mentioning

confidence: 99%

Toward Rapid Detection of Viable Bacteria in Whole Blood for Early Sepsis Diagnostics and Susceptibility Testing

et al. 2021

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Sepsis is a serious bloodstream infection where the immunity of the host body is compromised, leading to organ failure and death of the patient. In early sepsis, the concentration of bacteria is very low and the time of diagnosis is very critical since mortality increases exponentially with every hour after infection. Common culture-based methods fail in fast bacteria determination, while recent rapid diagnostic methods are expensive and prone to false positives. In this work, we present a sepsis kit for fast detection of bacteria in whole blood, here achieved by combining selective cell lysis and a sensitive colorimetric approach detecting as low as 10 3 CFU/mL bacteria in less than 5 h. Homemade selective cell lysis buffer (combination of saponin and sodium cholate) allows fast processing of whole blood in 5 min while maintaining bacteria alive (100% viability). After filtration, retained bacteria on filter paper are incubated under constant illumination with the electrochromic precursors, i.e., ferricyanide and ferric ammonium citrate. Viable bacteria metabolically reduce iron(III) complexes, initiating a photocatalytic cascade toward Prussian blue formation. As a proof of concept, we combine this method with antibiotic susceptibility testing to determine the minimum inhibitory concentration (MIC) using two antibiotics (ampicillin and gentamicin). Although this kit is used to demonstrate its applicability to sepsis, this approach is expected to impact other key sectors such as hygiene evaluation, microbial contaminated food/beverage, or UTI, among others.

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Fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics

Cited by 19 publications

References 22 publications

Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

Chromatic Toxicity Bioassay of Water through Bacterial Ferricyanide Reduction

Toward Rapid Detection of Viable Bacteria in Whole Blood for Early Sepsis Diagnostics and Susceptibility Testing

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