Fas receptor is not present on ejaculated human sperm

Perticarari, Sandra; Ricci, Giuseppe; Boscolo, Rita; Santis, Michela De; Pagnini, G.; Martinelli, Monica; Presani, Gianni

doi:10.1093/humrep/den113

Cited by 16 publications

(21 citation statements)

References 34 publications

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“…Caspase-8 was activated to a certain level, most probably as a result of caspase ''cross talk'' (Grunewald et al, 2005c;Paasch et al, 2004b). These results were confirmed in a very recent study (Perticarari et al, 2008) suggesting that spermatozoal mitochondria are extremely susceptible to specific agonists of apoptosis. Betulinic acid molecules are currently being used experimentally in anticancer treatments, their side effects on the male reproductive system should be carefully considered.…”

Section: Triggers and Inhibitors Of Caspase Activation In Human Spermsupporting

confidence: 66%

“…While recent studies deny the presence of the Fas receptors on human sperm (Perticarari et al, 2008), earlier studies detected it on the surface of subsets of ejaculated spermatozoa using various techniques like western blotting and immuncytochemistry (Grunewald et al, 2001;Sakkas et al, 1999). However, their functional impact is unclear.…”

Section: Triggers and Inhibitors Of Caspase Activation In Human Spermmentioning

confidence: 99%

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Impact of caspase activation in human spermatozoa

Grunewald

Sharma

Paasch

et al. 2009

Microscopy Res & Technique

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Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase-positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech. 00:000-000, 2009. V

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Section: Triggers and Inhibitors Of Caspase Activation In Human Spermsupporting

confidence: 66%

Section: Triggers and Inhibitors Of Caspase Activation In Human Spermmentioning

confidence: 99%

Impact of caspase activation in human spermatozoa

Grunewald

Sharma

Paasch

et al. 2009

Microscopy Res & Technique

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“…In the majority of the studies, Fas was not found on ejaculated sperm cells in normozoospermic men, [23][24][25] or if present, only in low percentages. 26 Some studies did not even observe Fas on spermatozoa from infertile men 23 or only at low percentages.…”

Section: Discussionmentioning

confidence: 93%

“…26 Some studies did not even observe Fas on spermatozoa from infertile men 23 or only at low percentages. 25 Studies have found Fas-positive sperm cells in infertile men, and reported inverse relationships between Fas and normal sperm cell morphology, concentration and motility. 14,24,26 These findings indicate that abortive apoptosis may have taken place.…”

Section: Discussionmentioning

confidence: 99%

Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics

Specht

Spanò²,

Hougaard³

et al. 2012

Asian J Androl

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Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P,0.01), more motile sperms (P50.04) and fewer sperm head defects (P50.05). These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone (FSH) and sexual hormone-binding globulin (SHBG) were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.

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“…In addition, the authors suggested that the Fas system is important in eliminating defective spermatozoa, but can show irregularities in sperm quality [27]. Also, Perticarari et al explained that the presence of Fas on ejaculated sperm is due to abortive apoptosis [60].…”

Section: Discussionmentioning

confidence: 99%

Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

Giudice

Lima

Cenedeze

et al. 2010

J Assist Reprod Genet

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Purpose To assess FasL mRNA levels in ejaculated sperm from adolescent patients with and without varicocele. Methods Semen was obtained by masturbation following 2-4 days of ejaculatory abstinence, from 14 adolescents with varicocele grades II and III (study group), and 20 adolescents without varicocele (control group). Seminal analysis was done according to World Health Organization guidelines and morphology using Kruger's strict criteria. The Fas-ligand (FasL) gene expression was performed using reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR) analysis, according to the expression level of the housekeeping cyclophilin A gene. A Student's t-test was applied to compare the groups, and Spearman's rank test in order to verify possible correlations (p<0.05).Results Quantitative RQ-PCR demonstrated that the expression of FasL mRNA in sperm from the varicocele group was higher than in the control group. Also, sperm concentration was higher in the controls, when compared to the varicocele group. When submitted to correlation analysis, adolescents with varicocele presented a correlation between sperm concentration and FasL gene expression levels (r=−0.470), not observed in controls. Conclusion Our results allow us to conclude that, in adolescents with varicocele presenting lower sperm concentration, FasL mRNA levels are higher than in adolescents without varicocele.

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Fas receptor is not present on ejaculated human sperm

Abstract: There is no detectable quantity of Fas on human ejaculated sperm.

Cited by 16 publications

References 34 publications

Impact of caspase activation in human spermatozoa

Impact of caspase activation in human spermatozoa

Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics

Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

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