FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis

Desjardins, Stuart D; Simmonds, James; Guterman, Inna; Kanyuka, Kostya; Burridge, Amanda J.; Tock, Andrew J.; Sanchez-Moran, Eugenio; Franklin, F. Christopher H.; Henderson, Ian R.; Edwards, Keith J; Uauy, Cristobal; Higgins, James D.

doi:10.1038/s41467-022-31438-6

Cited by 2 publications

(88 citation statements)

References 61 publications

(88 reference statements)

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“…A VIGS approach has been utilised to analyse the effect of FANCM hypomorphs on recombination in F 1 tetraploid wheat hybrids, where silencing had no effect on CO numbers, but altered CO distributions [ 55 ]. We have recently shown that FANCM promotes formation of class I COs, whilst preventing the formation of class II COs in tetraploid and hexaploid wheat [ 56 ]. In tetraploid wheat, the gain in class II COs is equal to the loss of class I COs, thus no net change in recombination occurred in fancm .…”

Section: How Do Dna Repair Genes Modulate Recombination?mentioning

confidence: 99%

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Unravelling mechanisms that govern meiotic crossover formation in wheat

Higgins

Osman

Desjardins

et al. 2022

Biochemical Society Transactions

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Wheat is a major cereal crop that possesses a large allopolyploid genome formed through hybridisation of tetraploid and diploid progenitors. During meiosis, crossovers (COs) are constrained in number to 1–3 per chromosome pair that are predominantly located towards the chromosome ends. This reduces the probability of advantageous traits recombining onto the same chromosome, thus limiting breeding. Therefore, understanding the underlying factors controlling meiotic recombination may provide strategies to unlock the genetic potential in wheat. In this mini-review, we will discuss the factors associated with restricted CO formation in wheat, such as timing of meiotic events, chromatin organisation, pre-meiotic DNA replication and dosage of CO genes, as a means to modulate recombination.

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Section: How Do Dna Repair Genes Modulate Recombination?mentioning

confidence: 99%

“…However, in the hexaploid fancm null mutant, the loss of class I COs is relatively less than the tetraploid, giving an overall 31% increase in COs. This gain in COs associates with H3K27me3 and the additional COs are likely to be in the same ‘hot’ regions as wild type [ 56 ].…”

Section: How Do Dna Repair Genes Modulate Recombination?mentioning

confidence: 99%

Unravelling mechanisms that govern meiotic crossover formation in wheat

Higgins

Osman

Desjardins

et al. 2022

Biochemical Society Transactions

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“…First, plants are more tolerant to harmful genome variations. For example, the mutation of chromosome recombination-related genes such as Fanconi Anemia Complementation Group M ( FANCM ) causes cancers and other severe syndromes in animals but does not cause obvious vegetative or reproductive growth defects in plants ( 10 , 11 ). Second, plants can produce a large number of descendants that allow us to screen large populations for rare mutants and infrequent characters.…”

Section: Introductionmentioning

confidence: 99%

Characterization and acceleration of genome shuffling and ploidy reduction in synthetic allopolyploids by genome sequencing and editing

Zhang

Liu

et al. 2022

Nucleic Acids Research

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Polyploidy and the subsequent ploidy reduction and genome shuffling are the major driving forces of genome evolution. Here, we revealed short-term allopolyploid genome evolution by sequencing a synthetic intergeneric hybrid (Raphanobrassica, RRCC). In this allotetraploid, the genome deletion was quick, while rearrangement was slow. The core and high-frequency genes tended to be retained while the specific and low-frequency genes tended to be deleted in the hybrid. The large-fragment deletions were enriched in the heterochromatin region and probably derived from chromosome breaks. The intergeneric translocations were primarily of short fragments dependent on homoeology, indicating a gene conversion origin. To accelerate genome shuffling, we developed an efficient genome editing platform for Raphanobrassica. By editing Fanconi Anemia Complementation Group M (FANCM) genes, homoeologous recombination, chromosome deletion and secondary meiosis with additional ploidy reduction were accelerated. FANCM was shown to be a checkpoint of meiosis and controller of ploidy stability. By simultaneously editing FLIP genes, gene conversion was precisely introduced, and mosaic genes were produced around the target site. This intergeneric hybrid and genome editing platform not only provides models that facilitate experimental evolution research by speeding up genome shuffling and conversion but also accelerates plant breeding by enhancing intergeneric genetic exchange and creating new genes.

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FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis

Cited by 2 publications

References 61 publications

Unravelling mechanisms that govern meiotic crossover formation in wheat

Unravelling mechanisms that govern meiotic crossover formation in wheat

Characterization and acceleration of genome shuffling and ploidy reduction in synthetic allopolyploids by genome sequencing and editing

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