False‐positive HLA antibody screen associated with Campath administration

Lyon, David C.; Hsu, Mei‐Yu; Colombe, Beth; Ballas, Samir K.

doi:10.1046/j.1537-2995.2001.41121626.x

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…Infliximab (anti-TNFa), commonly used in Crohn's disease, was tested in one report and was found to not interfere significantly with FC-XM [16]. Known FC-XM-interfering biologic agents are summarized in Table 1 [20][21][22][23][24][25][26][27][28][29]. Anti-thymocyte globulin (ATG, rabbit) Thymoglobulin…”

Section: Known Biologic Interferersmentioning

confidence: 99%

Antibody Therapeutics as Interfering Agents in Flow Cytometry Crossmatch for Organ Transplantation

Kueht

Dongur

Mujtaba

et al. 2023

JPM

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Donor–recipient matching is a highly individualized and complex component of solid organ transplantation. Flowcytometry crossmatching (FC-XM) is an integral step in the matching process that is used to detect pre-formed deleterious anti-donor immunoglobulin. Despite high sensitivity in detecting cell-bound immunoglobulin, FC-XM is not able to determine the source or function of immunoglobulins detected. Monoclonal antibody therapeutic agents used in a clinic can interfere with the interpretation of FC-XM. We combined data from the prospectively maintained Antibody Society database and Human Protein Atlas with a comprehensive literature review of PubMed to summarize known FC-XM-interfering antibody therapeutics and identify potential interferers. We identified eight unique FC-XM-interfering antibody therapeutics. Rituximab (anti-CD20) was the most-cited agent. Daratumuab (anti-CD38) was the newest reported agent. We identified 43 unreported antibody therapeutics that may interfere with FC-XM. As antibody therapeutic agents become more common, identifying and mitigating FC-XM interference will likely become an increased focus for transplant centers.

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Section: Known Biologic Interferersmentioning

confidence: 99%

Antibody Therapeutics as Interfering Agents in Flow Cytometry Crossmatch for Organ Transplantation

Kueht

Dongur

Mujtaba

et al. 2023

JPM

View full text Add to dashboard Cite

show abstract

“…Non-HLA antibodies (e.g., autoantibody) may also bind to the cells producing a positive result (Lyon et al, 2002). Most notably, nonspecific immunoglobulin binding to Fc receptors can be mistaken as a positive result.…”

Section: Flow Cytometrymentioning

confidence: 99%