Failure of Lymphocytes from Hypogammaglobulinaemic Subjects to Transform in Culture

Studies on the in vitro behavior of agammaglobulinemic lymphocytes

“…These data are in contrast to those reported by Fudenberg and Hirschhorn (15), Cline and Fudenberg (16), and Elves, Roath, and Israels (17). They have reported that lymphocytes have failed to proliferate upon stimulation with antigen or PHA, and failed to produce y-globulin.…”

The Potential of Lymphocytes from Patients with Leukaemia and Reticuloses to Transform under the Influence of Phytohaemagglutinin

“…In an earlier report we described such a normal degree of transformation (7), and more recently we have also described a low degree of transformation in a group of patients with this disease who had the complication of hypogammaglobulinaemia (8). Others have regularly found a decreased degree of transformation in cultures of lymphocytes from patients with this type of leukaemia (1,2,3,16,19,21).…”

Impairment of Phytohaemagglutinin-Induced Blastic Transformation in Lymphnodes from Thymectomized Mice

“…It is pertinent, however, that some lymphoid populations exhibiting a greatly decreased immu nological reactivity, such as lymphocytes from the thymus (12,48,76,82) or from the blood of patients with chronic lymphatic leukaemia (4,8,56,58,63,71,77,81), Hodgkin's disease (2,35), ataxia-telangiectasia (59), or with certain types of agamma-D ukor/D ietrich, Impairm ent of Phytohaemagglutinin-Induced globulinacmia (13,27) have repeatedly been found to respond very poorly to PHA in vitro. Moreover, peripheral blood lymphocytes from patients undergoing immunosuppressive chemotherapy were shown to exhibit a greatly reduced transformation rate following exposure to PHA (36).…”