Faecal leukocyte esterase activity is an alternative biomarker in inflammatory bowel disease

Abstract

Background: Leukocyte cytosolic proteins (e.g., calprotectin) are emerging biomarkers for inflammatory bowel disease. Leukocyte aryl esterase activity has been commonly used for sensitive detection of leukocytes in human body fluids such as urine. Urine test strip results are generally reported in categories. As automated strip readers allow quantitative data to be reported, sensitive quantitative detection of leukocytes in body fluids has become possible. Here, we explored the use of leukocyte esterase as a potential alternative faecal biomarker for inflammatory bowel disease. Methods: We evaluated leukocyte esterase activity in faecal extracts and compared Cobas u 411 (Roche) quantitative reflectance data with calprotectin concentration for 107 routine samples. Stability of leukocyte esterase for trypsin digestion was carried out by adding trypsin to the extract. Incubation occurred at 37 °C for 24 h or 48 h. Results: Reproducibility of the reflectance signal was good (within-run imprecision: 6.1%; between-run imprecision: 6.2%). Results were linear in the range 10

Serum platelet factor 4 is a reliable activity parameter in adult patients with inflammatory bowel disease

“…Nevertheless, these results suggest that measuring serum PF4 is feasible, and the encouraging data aid designing future studies. Moreover, since stool markers like calprotectin have become a standard in IBD disease monitoring, [11] comparisons between serum PF4 and stool markers should been made. A modified Mayo score and the Harvey–Bradshaw Index were used as indicators of UC disease activity and CD disease activity, respectively, but they also have drawbacks and may be affected by the subjective descriptions provided by patients.…”

Translational safety biomarkers of colonic barrier integrity in the rat

“…Calprotectin in feces (stored at −20°C) was measured according to the manufacturer's instructions, using the same assay as for serum. Similar as described by Dumoulin et al (), fecal leukocyte esterase was determined on the Urisys 2400 instrument using the same fecal extracts generated for calprotectin analysis. Routine hematology and clinical chemistry profiles were measured using the automated analyzers listed in Table .…”

“…d ‐Lactate was included as an endogenous permeability biomarker (Sun et al, ). In addition, metabolomics, routine hematology and clinical chemistry, and the inflammatory or damage‐related biomarkers α 2 ‐macroglobulin, calprotectin, a panel of cytokines, fecal occult blood, leukocyte esterase and a panel of prostaglandins were used to assess intestinal barrier integrity (Cury et al, ; Dumoulin et al, ; Lambert, ; Lied et al, ; Martin et al, ).…”

Quantitative urine test strip reading for leukocyte esterase and hemoglobin peroxidase

“…Leukocyte esterase is a useful marker for cells of granulocytic origin [11,12]. The leukocyte test is based on the classical hydrolysis of an amino acid ester by leukocyte esterase to its corresponding alcohol, which reacts with a diazonium salt to produce a violet azo-dye [13].…”