Factors influencing the development of dental erosion in vitro: enamel type, temperature and exposure time

Abstract

The influence of temperature, duration of exposure, and enamel type on the development and progression of dental erosion has been determined. Three experiments were devised as follows. Eroded lesions were produced on enamel samples with orange juice: (1) at different temperatures; (2) for different lengths of time; and (3) on bovine permanent, human deciduous and human permanent enamel. Lesion parameters (mineral loss and lesion depth) were quantified using transverse microradiography. Both lesion parameters were significantly lower at 4 degrees C when compared with 20 degrees C and 37 degrees C, and at 20 degrees C when compared with 37 degrees C. Lesion parameters increased significantly as the length of exposure increased, and were positively correlated (r=0.98, P<0.05) to the exposure time. Both parameters were significantly greater in bovine enamel than human permanent and deciduous enamel, and in human deciduous than permanent enamel. Lesion progression, as measured by mineral loss, was in the ratio 2.0:1.5:1.0 for bovine:human deciduous:human permanent, and by lesion depth, 1.7:1. 3:1.0. In conclusion, the erosiveness of orange juice was less pronounced at a lower temperature, and increased with an increased exposure time. Erosion progressed twice as fast in bovine permanent than in human permanent enamel, and 1.5 times more rapidly in human deciduous than in permanent enamel.

Viability of using enamel and dentin from bovine origin as a substitute for human counterparts in an intraoral erosion model

“…This result corroborates previous observations by Meurman and Frank (9), who did not find any noticeable morphological difference between eroded enamel specimens originated from cattle or human beings. On the other hand, the present finding is inconsistent with the results of an in vitro study in which progression of erosive lesions in bovine enamel was observed to occur twice faster (10). This may be ascribed to the fact that although bovine enamel may have been more susceptible to demineralization due to its wider interprismatic region, intraorally this structural difference may not have played a role.…”

“…While in an in vitro study (9) surface ultrastructure of bovine and human enamel following erosive episodes was indistinguishable, in another investigation eroded lesions have been shown to progress twice as fast in bovine than in human enamel (10). As a result, enamel originated from cattle has been suggested to wear more than human enamel under laboratory conditions (11).…”

Effect of foods and drinks on primary tooth enamel after erosive challenge with hydrochloric acid

“…Although it is a weak acid, citric acid chelates calcium from hydroxyapatite 18 and promotes the dissolution of crystals by binding the hydrogen ion to the carbonate and/or phosphate ion. 22 Although the manufacturer does not mention the presence of acidulants (e.g., citric acid) or any other acids, fermented milk (Yakult ® ) has a pH of 3.67 and may cause superficial mineral loss in bovine surface enamel after 20 min of exposure, interspersed with exposure to artificial saliva.…”

“…Prior to the initial microhardness test, the specimens were immersed in artificial saliva as described by McKnight-Hanes and Whitford 17 and modified according to Amaechi et al 18 for 24 h at 37ºC.…”

Influência do laser de CO2 associado ao fluoreto estanhoso no controle da erosão induzida por ácido clorídrico em esmalte de dentes decíduos-análise do desgaste e da permeabilidade

“…Fragments were flatenned, polished and fixed in resin blocks. Two piece of tapes were placed on the surface, leaving an exposed area 3x1mm 2 . For the formation of erosion lesions, specimens were submitted to an acid challenge by 4 cycles of 2 minutes each, with 0.01 M hydrochloric acid solution (pH 2).…”