Factors influencing regeneration and Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris L.)

Mukeshimana, Gerardine; Ma, Yu-Min; Walworth, Aaron E.; Song, Guo Qing; Kelly, James D.

doi:10.1007/s11816-012-0237-0

Cited by 27 publications

(60 citation statements)

References 53 publications

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“…In the present study, an efficient regeneration procedure based on multiple bud formation from epicotyl sections was developed. This result, in agreement with Varisai Mohamed et al Mukeshimana et al 2013) where shoots were regenerated from embryonic axis and from calli from cotyledonary nodes and apical meristems (Arellano et al 2009), this system is based on the production of multiple buds from non-meristematic tissue. The regeneration efficiency that we report is high compared with the results obtained previously by Delgado-Sánchez et al (2006), Gatica Arias et al (2010), and similar to those reported by Kwapata et al (2012) and Quintero-Jiménez et al (2010).…”

Section: Discussionsupporting

confidence: 91%

“…Regeneration Currently, an efficient transformation protocol for common bean demands that a regeneration system be developed using non-meristem-containing tissues as explants (Mukeshimana et al 2013). However, an efficient regeneration system for common bean using non-meristem-containing tissues as explants is a challenge (Mukeshimana et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…However, an efficient regeneration system for common bean using non-meristem-containing tissues as explants is a challenge (Mukeshimana et al 2013). In the present study, an efficient regeneration procedure based on multiple bud formation from epicotyl sections was developed.…”

Section: Discussionmentioning

confidence: 99%

“…However, Agrobacterium inoculation contributed to detrimental effects on common bean regeneration. The recent attempts to transform common bean using Agrobacterium failed (Amugune et al 2011;Mukeshimana et al 2013), and transformed tissue could not be recovered mainly due to the poor regeneration. In this study, after Agrobacterium inoculation the percentage of multiple bud formation diminished from 65.5 to 32.8%.…”

Section: Discussionmentioning

confidence: 99%

“…Even though the recovered putative transgenic plants were chimeras, this established protocol shows the advantage of being efficient for the regeneration of transformed common bean tissue. Since none of the published common bean transformation procedures using Agrobacterium have recovered transformed plants-either chimeric or completely transgenicdue to the inefficient regeneration (Amugune et al 2011;Mukeshimana et al 2013), the protocol described here is an advance compared with the previous attempts above. This is an important step concerning Agrobacterium-mediated transformation in P. vulgaris and it can also be used to assist follow-up research regarding genetic transformation and breeding of this important legume species.…”

Section: Discussionmentioning

confidence: 99%

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Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Collado

Bermúdez-Caraballoso

García

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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Common bean is recalcitrant to genetic transformation, due to limited regeneration capacity and low DNA transfer rates. The effect of different parameters on T-DNA transfer from Agrobacterium tumefaciens, was studied by measuring transient expression of the β-glucuronidase gene in Phaseolus vulgaris L. cv. CIAP7247F. Epicotyl containing seedling explants were inoculated with Agrobacterium EHA101 and C58C1Rif R (pMP90) strains harboring the binary vector pTJK136 with GusA gene on the T-DNA. Parameters studied were temperature and light regime during co-cultivation, explant injury, and the acetosyringone concentration for vir gene induction. The co-cultivation temperature and photoperiod had a significant effect on Agrobacterium DNA transfer. In addition, explant injury and supplementation of the cocultivation medium with acetosyringone increased the GUS activity. Optimal T-DNA transfer was obtained under the following conditions: co-cultivation at 25°C in darkness, injuring the explants with carborundum, and supplementation of the co-cultivation medium with 200 μM acetosyringone. This T-DNA delivery system was combined with a direct organogenesis protocol using epicotyl explants and fertile regenerants were recovered from tissue transformed with Agrobacterium. However, no transmission of transgenes to progeny could be observed, suggesting that the obtained plants were chimeras.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Collado

Bermúdez-Caraballoso

García

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

Huertas

Karpińska

Ngala

et al. 2022

Food and Energy Security

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Micronutrient deficiencies (hidden hunger), particularly in iron (Fe) and zinc (Zn), remain one of the most serious public health challenges, affecting more than three billion people globally. A number of strategies are used to ameliorate the problem of micronutrient deficiencies and to improve the nutritional profile of food products. These include (i) dietary diversification, (ii) industrial food fortification and supplements, (iii) agronomic approaches including soil mineral fertilisation, bioinoculants and crop rotations, and (iv) biofortification through the implementation of biotechnology including gene editing and plant breeding.These efforts must consider the dietary patterns and culinary preferences of the consumer and stakeholder acceptance of new biofortified varieties. Deficiencies in Zn and Fe are often linked to the poor nutritional status of agricultural soils, resulting in low amounts and/or poor availability of these nutrients in staple food crops such as common bean. This review describes the genes and processes associated with Fe and Zn accumulation in common bean, a significant food source in Africa that plays an important role in nutritional security. We discuss the conventional plant breeding, transgenic and gene editing approaches that are being deployed to improve Fe and Zn accumulation in beans. We also consider the requirements of successful bean biofortification programmes, highlighting gaps in current knowledge, possible solutions and future perspectives.

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CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges

et al. 2021

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Legumes are an excellent source of proteins and health‐promoting phytochemicals. Recognizing their importance in human nutrition and sustainable agricultural production, significant efforts are currently being made to accelerate genetic gain related to yield, stress resilience, and nutritional quality. Recent increases in genomic resources for multiple legume crops have laid a solid foundation for application of transformative breeding technologies such as genomic selection and genome editing for crop improvement. In this review, we focus on the recent plant‐specific advances in CRISPR/Cas9‐based gene editing technology and discuss the challenges and opportunities to harnessing this innovative technology for targeted improvement of traits in legume crops. Gene‐editing methods have been successfully established for soybean, cowpea, chickpea, and model legumes such as Medicago truncatula and Lotus japonicus. However, the recalcitrance of other legumes to in vitro gene transfer and regeneration has posed a serious challenge to application of gene editing. We discuss various modifications to in vitro culture methods, in terms of the choice of explant, media composition, and DNA delivery and gene‐editing detection methods that can potentially improve the rate of transformation and regeneration of whole plant in legume crops. Although gene‐editing technology can bring enormous benefits to legume breeding, regulatory hurdles are a cause for serious concern. We compare the regulatory environments existing in the European Union and the United States of America. A favorable regulatory framework and public acceptance are important factors in realizing CRISPR's potential benefits to global food security.

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Factors influencing regeneration and Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris L.)

Cited by 27 publications

References 53 publications

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges

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