2021
DOI: 10.33448/rsd-v10i2.12264
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Factors affecting the in vitro production of bovine embryos in a commercial program

Abstract: Variability in the production of bovine blastocysts per session of ultrasound-guided follicularaspiration(OPU) is still an obstacle in commercial production.Thus, the objective of the current work was to verify factors that influence in vitro embryo production (PIVE), using data from a commercial laboratory.Data from 2014 to 2016 were analyzed, referring to 799 OPU sessions of adult bovine females of the breeds: Nelore (n=83), Girolando (n=73), Brangus (n=49), HPB (n=20), and Senepol (n=10).The influence of fi… Show more

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Cited by 5 publications
(6 citation statements)
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References 30 publications
(31 reference statements)
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“…In Black Japanese cows in the hot (28.3 ± 0.3 °C, 66.5% RH) and cool (17.5 °C, 62.9% RH) seasons, 11.2 ± 0.8 and 23.2 ± 1.9 COCsT were obtained by OPU [35] and in Gyr cows for 14 days in controlled environments at 25 and 38 °C, 11.2 ± 2.8 and 14.3 ± 2.5 COCsT were obtained [36]. In addition to climatological and environmental factors, genotype, aspiration frequency and semen used, among others, affect COCsT in an OPU-IVF program, [37]. In this for each kg of increase in BW of the donors, the COCsT increased with 𝛽 ̂= 0.002±0.001, well fed donors have greater energy reserves that are used in reproductive events [38].…”
Section: Discussionmentioning
confidence: 92%
“…In Black Japanese cows in the hot (28.3 ± 0.3 °C, 66.5% RH) and cool (17.5 °C, 62.9% RH) seasons, 11.2 ± 0.8 and 23.2 ± 1.9 COCsT were obtained by OPU [35] and in Gyr cows for 14 days in controlled environments at 25 and 38 °C, 11.2 ± 2.8 and 14.3 ± 2.5 COCsT were obtained [36]. In addition to climatological and environmental factors, genotype, aspiration frequency and semen used, among others, affect COCsT in an OPU-IVF program, [37]. In this for each kg of increase in BW of the donors, the COCsT increased with 𝛽 ̂= 0.002±0.001, well fed donors have greater energy reserves that are used in reproductive events [38].…”
Section: Discussionmentioning
confidence: 92%
“…The recovery of grade C oocytes was higher than grades A, B, D and E oocytes in the present study. Results reported by Looney et al (1994), Manik et al (2003), Chaubal et al (2006), Sakhong et al (2012) Nogueira et al (2021), breed, blood group and frequency of aspirations have a strong influence on embryonic production. Wilson et al (2006) produced more number of grade 2 embryos followed by grade 1 and grade 3 respectively in contrast to the present study.…”
Section: Grading Of Embryosmentioning
confidence: 86%
“…Sperm straws were thawed for 30 seconds in 37  C water after being frozen. After thawing, a discontinuous Percoll gradient was used (Sigma GE17-0891-01) (45 and 90% (vol/vol) Percoll) (Maziero et al, 2020;Nogueira et al, 2021). Semen was inserted in a 15 mL tube with 5 ml capacitation medium.…”
Section: In Vitro Fertilization Of Oocytesmentioning
confidence: 99%
“…The final stage of in vitro production (IVP) is the in vitro culturing of bovine embryos, which begins with a zygote culture and normally takes 7 to 8 days. At this stage, the embryo undergoes its first cleavage division, the embryonic genome is activated and the morula and blastocyst are created (W renzycki, 2018;Ramos-Deus et al, 2020;Nogueira et al, 2021).…”
Section: Introductionmentioning
confidence: 99%