Factors Affecting Genetic Resistance of Mice to Mouse Typhoid

Gowen, John W.; Calhoun, M. Lois

doi:10.1093/infdis/73.1.40

Cited by 28 publications

(10 citation statements)

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“…The major histocompatibility complex appears to play only a minor role, if any, in host resistance to bacterial infection. Resistance to infection by Corynebacterium kutscheri (12,17), Mycobacterium lepraemurium (6, 7, 13), Mycobacterium tuberculosis (1), Listeria monocytogenes (4,5,8,22), Rickettsia tsutsugamushi, Rickettsia akari (2,9), and Salmonella typhimurium (8, 14, 15, 18-20) has been demonstrated to be genetically regulated. In general, non-H-2 genes have the predominate role in regulation of host resistance to other organisms.…”

Section: Discussionmentioning

confidence: 99%

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Genetic studies of the murine corneal response to Pseudomonas aeruginosa

Berk

Leon

Hazlett³

1981

Infect Immun

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The murine genetic control of resistance to Pseudomonas aeruginosa eye infection previously has been demonstrated to be regulated by two complementing dominant genes, PsCRJ and PsCR2. The PsCRJ locus apparently is not associated with the H-2 complex, whereas the PsCR2 locus could not definitively be associated with H-2. In this study we attempted to demonstrate a possible H-2 linkage of the PsCR2 locus. A panel of inbred congenic strains varying with either the H-2 haplotype or genetic background from inbred partners of C57BL/10, C3H, A, and BALB/c strains were characterized for their P. aeruginosa infectivity phenotypes. These studies indicated that the PsCR2 locus is not associated with the H-2 locus. Furthernore, variations of the H-2 haplotype did not change the resistance patterns observed in these strains. However, BALB.B and BALB.K congenic lines were resistant to P. aeruginosa eye infection, whereas BALB/cJ mice were susceptible. Examination of hybrids (BALB.K x BALB/cJ)Fl and (BALB.B x BALB/cJ)Fl demonstrated that an autosomal dominant gene(s), PsCR, confers resistance. Segregation analysis for the H-2 haplotype and the PsCR gene in offspring of backcross matings with the BALB/cJ parental strain suggested that this PsCR gene is not linked to the H-2 complex and has an inheritance pattem of a single locus or several tightly linked loci.

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Section: Discussionmentioning

confidence: 99%

“…The use of inbred mice by some investigators has led to an increased interest in genetic control of natural resistance to infection (2, 4,6,8,12). However, there appears to be virtually nothing in the literature on genetic control of eye infections in experimental animals.…”

mentioning

confidence: 99%

Genetic studies of the murine corneal response to Pseudomonas aeruginosa

Berk

Leon

Hazlett³

1981

Infect Immun

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“…reported a peripheral granulocytosis in 3 to 4 month old mice 24 hours after 10/zg of S. typhosa endotoxin given intraperitoneally correlated with increased resistance to a pseudomonad infection, although repeated endotoxin treatment produced a diminution in white blood cells closely approaching the normal control level while the increased resistance still persisted (13). In studying factors concerned with innate rather than acquired immunity, Gowen and Calhoun found the degree of resistance to S. ~yphimurium in selected, resistant inbred mice to be correlated with the mean number of circulating leucocytes (14). But Weir, et al found mice bred for low leucocyte counts to be more resistant to the same mouse pathogen (15).…”

Section: Discussionmentioning

confidence: 99%

The Stimulation of Non-Specific Host Resistance to Infection by Chemically Modified Endotoxin

Sultzer

Freedman

1962

The Journal of Experimental Medicine

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In recent years the use of endotoxins to alter the natural equilibrium between host and parasite has engendered m a n y intriguing questions. One such problem of primary interest is whether the lethal and pyrogenic effects of these materials are necessarily related to their property of increasing resistance of the host to infection. With the development of a relatively detoxified endotoxin, this subject became amenable to direct investigation (1, 2).The experiments reported in this paper deal with several properties of the detoxified derivative in stimulating host defense mechanisms as directly compared to the original endotoxin. The essentially equivalent capacity to acutely increase resistance to various bacterial infections has been demonstrated, as well as the differences produced b y varying the methods of treatment and infection. Studies dealing with the passive transfer of protection and the effects on the level of circulating leucocytes in the mouse are also discussed. Materials and MethodsAnimals.--Male mice of the ICR strain weighing 16 to 18 gm (Bellewood Farm, Englishtown, New Jersey) were housed 10 to a metal cage, fed Purina lab chow and water ad libitum. All animals were used routinely within 1 week after receipt.Bacterial Cultures.--Three representative Gram-negative bacteria were employed, including strains of Escherichia coli and Salmonella typhimurium previously described (3) and a strain of Pseudomonas aeruginosa isolated in this laboratory. A strain of Staphylococcus aureus (Smith) known to be virulent for mice was kindly provided by Dr. Ian M. Smith. Each organism was grown in brain heart infusion broth (BHI) (Difco Laboratories, Inc., Detroit) for 18 hours at 35°C prior to each experiment. A standard inoculum was determined for E. coli, Ps. aeruginosa, and S. aureus which produced 70 per cent to 80 per cent mortality within 3 days in those animals injected intraperitoneally. In those mice infected with S. typhimurium, half the deaths would occur within I day, or, with a smaller inoculum, by 3 days. In certain experiments, an E. coli bacteremia was produced by intravenous injection of 0.25 ml of an 18 hour culture into one of the tail veins, a dose which produced 90 per cent to 100 per cent deaths within 3 days. For the Gram-negative cultures, appropriate dilutions were made in sterile BHI broth. The staphylococcal culture was centrifuged, the cells washed once, and resuspended to their original volume in sterile saline. Viable numbers of organisms were determined by triplicate plate counts on BHI agar. All inocula were kept chilled in an ice bath during the challenge procedure and 0.25 ml of the appropriate dilutions was used for intraperitoneal injections.

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“…(1,11) . É conhecido que ratos e camundongos podem exibir variações dos parâmetros bioquímicos relacionadas com sexo, linhagem e genótipo, podendo ser influenciados pela idade, pela dieta, pelo manuseio, pelo meio ambiente, entre outros fatores (8) . Por essa razão, o conhecimento dos valores dos diferentes parâmetros fisiológicos é critério importante para a avaliação da homeostase, das modificações induzidas por processos patológicos e dos resultados obtidos nos procedimentos experimentais (9) .…”

Section: Introductionunclassified