Factor VIII-related antigen in Kaposi's sarcoma in young homosexual men

Flotte, T. J.

doi:10.1001/archderm.120.2.180

Cited by 12 publications

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Cytomegalovirus infection and viral‐induced transformation of human endothelial cells

Smiley

Mar

Huang

1988

Journal of Medical Virology

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Human cytomegalovirus (CMV) has been associated with vascular pathology. In vivo, CMV is present in vessel wall cells during acute and chronic infections as well as in atherosclerotic lesions. CMV nucleic acids and proteins have also been detected within Kaposi's sarcoma lesions. Because of these associations, we studied the interaction of CMV with human endothelial cells with particular attention to its oncogenicity in this cell type. Our data demonstrate that human endothelial cells are permissive to viral replication but that the viral replication cycle is delayed compared with fibroblast cells. Persistent infections can result with minimal cytopathology. CMV can transform these cells to anchorage-independent growth, and noninfectious virus is still capable of inducing this transforming event. Our results demonstrate that productive or persistent CMV infection of endothelial cells and viral-induced transformation can occur, thus providing an in vitro correlate of in vivo events.

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Cytomegalovirus infection and viral‐induced transformation of human endothelial cells

Smiley

Mar

Huang

1988

Journal of Medical Virology

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Kaposi's sarcoma: Report of five cases treated with radiotherapy

Ampil

1986

Journal of Surgical Oncology

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An immunohistological study of factor VIII related antigen and kaposi's sarcoma using polyclonal and monoclonal antibodies

Millard

Heryet

1985

The Journal of Pathology

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Immunohistochemical techniques have been used to demonstrate Factor VIII related antigen (FVIIIRA) in endothelial cells and to study a variety of tumours including Kaposi's sarcoma. Conflicting reports on the presence of this antigen within the spindle cells of Kaposi's sarcoma have been made. These differences may be due to variations in techniques, in the immunoreagents used and in the method of fixation and handling of the specimen. A major factor may also be the lack of a uniform interpretation of positive peroxidase labelling. This study was confined to paraffin-embedded tissues and compared the results and distribution of FVIIIRA labelling in Kaposi's sarcoma using two commercially available polyclonal antibodies and three monoclonal antibodies. The effects of predigesting the sections with trypsin and protease were also evaluated. Positive labelling of tumour spindle cells was accepted only when it matched that seen in labelled endothelial cells. The polyclonal antibodies provided satisfactory localization of FVIIIRA and gave positive results more frequently than the monoclonal antibodies both with and without enzyme digestion. Endothelial labelling was evident in vessels both within and around the tumour but none was seen in the tumour spindle cells. Most previous studies gave no definition of positive labelling but spindle cells were reported as labelled. However, in those few reports where a definition was given the findings are analogous to those in this report, demonstrating the importance of defining positive labelling.

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Factor VIII-related antigen in Kaposi's sarcoma in young homosexual men

Cited by 12 publications

References 0 publications

Cytomegalovirus infection and viral‐induced transformation of human endothelial cells

Cytomegalovirus infection and viral‐induced transformation of human endothelial cells

Kaposi's sarcoma: Report of five cases treated with radiotherapy

An immunohistological study of factor VIII related antigen and kaposi's sarcoma using polyclonal and monoclonal antibodies

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