Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment

Huang, Chen; Zhan, Yunni; Cheng, Jinyuan; Wang, Jia; Meng, Xianzhi; Zhou, Xuelian; Fang, Guigan; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2021.124696

Cited by 62 publications

(20 citation statements)

References 24 publications

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“…Recently, Huang et al. reported a DES using guaiacol as the HBD with AlCl 3 as a catalyst [18] . Our group proposed to use p ‐hydroxybenzoic acid (PB), an atypical aromatic unit found in certain hardwood lignins (e. g., poplar, willow, and aspen), as a DES component to pretreat hardwood and successfully delignified poplar wood without additional catalyst [19] …”

Section: Introductionmentioning

confidence: 99%

Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent

et al. 2021

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Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. Herein, we leveraged a plant genetic engineering approach to specifically incorporate C6C1 monomers into the lignin structure. By expressing the bacterial ubiC gene in sorghum, p‐hydroxybenzoic acid (PB)‐rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild‐type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB‐rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (∼190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the β‐Ο‐4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB‐based DES and engineered PB‐rich biomass is a promising strategy to achieve a sustainable closed‐loop biorefinery.

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Section: Introductionmentioning

confidence: 99%

Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent

et al. 2021

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“…BL-oil was a complex organic solvent composed of 22.78% acetic acid, 6.11% phenol, and other organic hydrogen donors, and it can be mixed with ChCl to pretreat CS. It was deduced that acidic donors might combine with ChCl to absorb the OH-groups of phenol, thus forming a large amount of strong anionic and neutral hydrogen bonds. , The mixed system had strong reactivity to provide protons for the depolymerization of lignocellulose. Moreover, the destruction of the cross-linking structure further exposed the structural ash bonding in the physical structure of ligncellulose .…”

Section: Resultsmentioning

confidence: 99%

Pretreatment Influence of an Imitative Deep Eutectic Solvent Composed of Biomass Light Oil and Choline Chloride on Boosting Selective Saccharification during Corn Stalk Pyrolysis

Zhang

et al. 2021

ACS Sustainable Chem. Eng.

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As a novel pretreatment method, a deepeutectic solvent (DES) can effectively deconstruct lignocellulose to promote its selective saccharification. In this research, biomass light oil (BL-oil) and choline chloride (ChCl) were employed to prepare a novel, low-cost imitative DES to boost pyrolytic saccharification of corn stalk (CS). The physical−chemical properties of original CS and pretreated samples were investigated. Furthermore, the influence of mass ratio of BL-oil and ChCl as well as pretreated temperature on pyrolytic product distribution was studied. The results showed that the imitative DES had a similar pretreated effect to commercial DESs, which can destroy the structure of CS to facilitate the degradation of hemicellulose and lignin. In addition, the imitative DES was more conducive to removal of alkali metals and alkaline earth metals. The yield of levoglucosan increased from 2.59 to 59.87 mg/g under the optimum imitative DES pretreated condition. The preparation and utilization of a biomass-derived imitative DES broadened the application of biomass pyrolysis oil and realized the "closed-loop" concept of biorefinery.

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Section: Introductionmentioning

confidence: 99%

“…Deep eutectic solvents (DESs) have recently been considered as a novel solvent system to pretreat lignocellulosic biomass for lignin isolation and subsequent polysaccharide saccharification. 7 DESs emerge as an alternative to ionic liquid, but compared with ionic liquid, they feature designability, biocompatibility, biodegradability, and recyclability. During DES fractionation, the inherent H-bond interactions of DES render its strong competition with the inherent linkages in lignocellulose, thus leading to the fractionation of biomass.…”

Section: ■ Introductionmentioning

confidence: 99%

Direct Extraction of Uniform Lignin Microspheres from Bamboo Using a Polyol-Based Deep Eutectic Solvent

Cheng

Huang

Liu

et al. 2023

ACS Sustainable Chem. Eng.

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Conventional preparation of lignin micro-/nanoparticles (LMNPs) often needs multiple steps accompanied by high cost, low efficiency, and low yield. Herein, this study established a polyhydric alcohol-based deep eutectic solvent (PA-DES) fractionation for direct LMNP assembly from raw lignocellulose. The results indicated that lignin was significantly extracted by 41.55−70.33% depending on the PA-DES varieties. With lignin removal, the substrate showed high glucan enzymatic saccharification from 70.44 to 100%. Specially, over 90% lignin could be recovered in each PA-DES, and the obtained LMNPs showed quite uniform spherical morphology with a tunable size of 550−3254 nm and narrow polydispersity index (PDI) of 0.295−0.398. The proposed DES showed a near theoretical recovery yield, which still featured excellent fractionation performance even after three cycles of reuse. The findings highlight the simultaneous utilization of cellulose and lignin in a one-pot biomass fractionation under mild conditions with sugars and LMNPs as the coproducts.

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Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment

Cited by 62 publications

References 24 publications

Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent

Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p‐Hydroxybenzoic Acid‐Based Deep Eutectic Solvent

Pretreatment Influence of an Imitative Deep Eutectic Solvent Composed of Biomass Light Oil and Choline Chloride on Boosting Selective Saccharification during Corn Stalk Pyrolysis

Direct Extraction of Uniform Lignin Microspheres from Bamboo Using a Polyol-Based Deep Eutectic Solvent

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