Facile preparation of MIL-88B-Fe metal–organic framework with high peroxidase-like activity for colorimetric detection of hydrogen peroxide in milk and beer

Abstract

Hydrogen peroxide (H 2 O 2 ) was widely applied as the bactericide and bleach agent in food industry due to its strong oxidation, whose residue had also posed a hazard to public health at the same time. Therefore, it was indispensable to establish a simple, highly effective and accurate method for the detection of H 2 O 2 in foods. Herein, the MIL-88B-Fe metal-organic framework (MOF) was prepared by a facile solvothermal process and utilized as the peroxidase mimic for H 2 O 2 detection. The resultant MIL-88B-Fe MOF exhibited an excellent peroxidase mimetic activity to effectively catalyse the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H 2 O 2 , generating a blue coloured product. Under the optimal testing conditions, the linear absorbance response is well correlated with the H 2 O 2 concentration ranging from 10 to 100 μM with a detection limit of 0.60 μM. The catalytic kinetic assay suggested that the MIL-88B-Fe MOF possessed the higher affinity towards both the substrate TMB and H 2 O 2 than horseradish peroxidase. Moreover, the MIL-88B-Fe MOF also displayed a high selectivity and anti-interference performance towards H 2 O 2 detection. In addition, the peroxidase mimetic catalytic oxidation TMB mechanism over the MIL-88B-Fe MOF was clarified by the photoluminescence test. Benefitting from the sensitive response and good anti-interference capacity, this method was applied in real milk and beer samples for H 2 O 2 detection and showed a favourable reproducibility and feasibility. These results indicated that the proposed method based on MIL-88B-Fe as peroxidase mimic might be a promising candidate for H 2 O 2 detection in food field.

A highly efficient metal oxide incorporated metal organic framework [Nd2O3-MIL(Fe)-88A] for the electrochemical detection of dichlorvos

“…3d, and they reveal two distinct peaks at 725.3 and 712.0 eV, which stand for the binding energies of Fe 2p 1/2 and Fe 2p 3/2 , respectively. 44,46 The spin–orbit peaks for Nd 3d 5/2 and Nd 3d 3/2 are oriented at 982.5 and 1004.1 eV, respectively 45 (Fig. 3e).…”

“…3b) were deconvoluted into three peaks at 288.5, 286.1 and 284.6 eV, corresponding to O–CO, C–O–C and C–C, respectively. 40,44 The O 1s displayed in Fig. 3c at 533.4, 531.9 and 530.1 eV corresponds to O–H, C–O–Fe & Nd–O and Fe–O–Fe, respectively.…”

“…3c at 533.4, 531.9 and 530.1 eV corresponds to O–H, C–O–Fe & Nd–O and Fe–O–Fe, respectively. 44,45 The Fe 2p core-level spectra of Nd 2 O 3 @MIL (Fe)-88A are depicted in Fig. 3d, and they reveal two distinct peaks at 725.3 and 712.0 eV, which stand for the binding energies of Fe 2p 1/2 and Fe 2p 3/2 , respectively.…”

Characterization and Gel Properties of Low-Molecular-Weight Carrageenans Prepared by Photocatalytic Degradation

“…We have reported the photocatalytic degradation of fucoidan and chondroitin sulfate with TiO 2 as well as H 2 O 2 , and the products of 3~5 kDa were obtained after a 6 h reaction [ 25 , 26 ]. As a strong oxidizing agent, H 2 O 2 could promote the oxidation degradation [ 37 ], but it is harmful to health and unfriendly to environment [ 38 ]. Moreover, its addition could increase the production cost.…”

Dual-mode sensing of hydrogen peroxide on self-assembled Ag nanoparticles anchored on polydopamine wrapping 2D Cu-MOFs

“…12 Xu et al fabricated a colorimetric sensor based on an MIL-88B-Fe MOF as a peroxidase mimic for H 2 O 2 with a low detection limit of 2 μM. 13 In order to eliminate the influence of external factors, such as background interference and material properties, on the accuracy of electrochemical or colorimetric assay, dual-signal determination systems have been employed in the field of analysis because they can self-calibrate the results. 14 Currently, electrochemical/colorimetric dual-signal systems have attracted increasing attention owing to their characteristics of high anti-interference ability and rapid reaction.…”