Facile Interfacial Electron Transfer of Hemoglobin

Zhou, Bo; Sun, Rong; Hu, Xiaofang; Wang, Lihua; Wu, Haiping; Song, Shiping; Chen, Fan

doi:10.3390/i6120303

Cited by 11 publications

(6 citation statements)

References 36 publications

(42 reference statements)

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“…Over recent years, carbon nanomaterials [11][12][13], metal oxides [14], metal nanoparticles [15], ionic liquids [16] and conducting polymers [17] have been utilized as immobilization matrices for Hb. In particular, the 2D carbon nanomaterial graphene (GR) exhibits electronic conductivity and thermal stability superior to other carbon nanoforms [18,19], and make it an ideal support material in the fabrication of biosensors [19,20].…”

Section: Introductionmentioning

confidence: 99%

Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide

Velusamy

Palanisamy

Chen

et al. 2017

Sensors and Actuators B: Chemical

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In the present work, we have investigated the electrochemical behavior and electrocatalysis of hemoglobin (Hb) immobilized on a glassy carbon electrode (GCE) modified with a graphenecellulose microfiber (GR-CMF) composite. The GR-CMF composite was characterized by scanning electron microscopy, elemental analysis, and Raman and Fourier transform infrared spectroscopy. Well-defined electrochemical redox characteristics of Hb were observed for Hb immobilized on a GR-CMF composite modified GCE, with a formal potential of-0.306 V and a peak to peak separation of approximately 67 mV. Due to the high biocompatibility of the GR-CMF composite, the electrochemical behavior of the Hb heme redox couple (Fe II /Fe III) was enhanced for Hb immobilized on the GR-CMF composite when compared to Hb immobilized on pristine GR. The heterogeneous electron transfer constant (ks) was calculated as 6.17 s-1 , and is higher than previously reported for Hb immobilized GR supports. The Hb immobilized GR-CMF composite modified electrode was used for the quantification of H2O2 under optimal conditions, and shows a wider linear amperometric response ranging from 0.05 to 926 µM. The limit of detection of the biosensor was 0.01 µM with the sensitivity of 0.49 µAµM-1 cm-2. The biosensor also showed high selectivity in the presence of the range of interfering compounds and exhibits good operational stability and practicality in the detection of H2O2.

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Section: Introductionmentioning

confidence: 99%

Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide

Velusamy

Palanisamy

Chen

et al. 2017

Sensors and Actuators B: Chemical

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“…An apparent activation energy Ea was calculated from the slope of the line to be 22.8 kJ·mol −1 , which is smaller compared with the activation energy for enzyme-catalyzed reactions (20-85 kJ·mol −1 ). One of the reasons for the small apparent activation energy is probably the catalytic effect of polyaniline on the oxidation of H 2 O 2 [12] . Also, this is why the operational potential of the biosensor can be controlled lower than that of other choline biosensors [1] .…”

Section: Effect Of Temperature On the Response Current Of The Biosensormentioning

confidence: 99%

“…Therefore, polyaniline could function as "moleculewires" to relay electron transfer [12] , which is in favor of the electron transfer in the enzyme-catalyzed reactions. Such properties of polyaniline offer a possibility for the oxidation of H 2 O 2 at lower potentials.…”

Section: Introductionmentioning

confidence: 99%

Bioelectrochemical response of a choline biosensor fabricated by using polyaniline

Ding

Shan

Yangshan

2009

Sci. China Ser. B-Chem.

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On the basis of the isoelectric point of an enzyme and the doping principle of conducting polymers, choline oxidase was doped in a polyaniline film to form a biosensor. The amperometric detection of choline is based on the oxidation of the H 2 O 2 enzymatically produced on the choline biosensor. The response current of the biosensor as a function of temperature was determined from 3 to 40℃. An apparent activation energy of 22.8 kJ·mol −1 was obtained. The biosensor had a wide linear response range from 5 × 10 −7 to 1 × 10 −4 M choline with a correlation coefficient of 0.9999 and a detection limit of 0.2 μM, and had a high sensitivity of 61.9 mA·M −1 ·cm −2 at 0.50 V and at pH 8.0. The apparent Michaelis constant and the optimum pH for the immobilized enzyme are 1.4 mM choline and 8.4, respectively, which are very close to those of choline oxidase in solution. The effect of selected organic compounds on the response of the choline biosensor was studied. choline, choline oxidase, biosensor, polyaniline matrix

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“…Ngamna et al have immobilized horseradish peroxidase poly(2methsulphonated polyaniline-5-sulphonic acid)/l-lysine composite [127]. Zhou et al have observed enhanced peroxidase activity in hemoglobin in sulfonated polyaniline on glassy carbon electrodes [128]. This result has been attributed to the facile interfacial transfer of hemoglobin mediated by sulphonated polyaniline.…”

Section: Enzymosensorsmentioning

confidence: 99%

Prospects of Organic Conducting Polymer Modified Electrodes: Enzymosensors

Singh

2012

International Journal of Electrochemistry

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Organic conducting polymer modified electrodes (OCPMEs) have emerged as potential candidates for electrochemical biosensors due to their easy preparation methods along with unique properties, like stability in air and being compatible with biological molecules in a neutral aqueous solution. OCPMEs are playing an important role in the improvement of public health and environment for the detection of desired analytes with high sensitivity and specificity. In this paper, we highlight the prospects of OCMEs-based electrochemical enzymosensors.

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Facile Interfacial Electron Transfer of Hemoglobin

Cited by 11 publications

References 36 publications

Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide

Graphene dispersed cellulose microfibers composite for efficient immobilization of hemoglobin and selective biosensor for detection of hydrogen peroxide

Bioelectrochemical response of a choline biosensor fabricated by using polyaniline

Prospects of Organic Conducting Polymer Modified Electrodes: Enzymosensors

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