Facile In-Situ Fabrication of a Ternary ZnO/TiO2/Ag Nanocomposite for Enhanced Bactericidal and Biocompatibility Properties

Abstract

This paper presents for the first time a successful fabrication of ternary ZnO/TiO2/Ag nanocomposites consisting of zinc oxide (ZnO), titania (TiO2) and silver (Ag) nanoparticles (NPs) synthesised using Morinda citrifolia fruit (MCF) extract. ZnONPs were synthesised using the co-precipitation method, and TiO2 and Ag were introduced into the precursor solutions under microwave irradiation to obtain ZnO/TiO2/Ag nanocomposites (NCs). This material demonstrated enhanced bactericidal effect towards bacterial pathogens compared to that of the binary TiO2/Ag, Ag and TiO2 alone. In vitro cytotoxicity results of the as-synthesised ZnO/TiO2/AgNCs on RAW 264.7 macrophages and A549 cell lines revealed a negative role in cytotoxicity, but contributed astoundingly towards antimicrobials as compared of Ag alone and binary Ag/TiO2. This study shows that the resultant ternary metal/bi-semiconductor nanocomposites may provide a therapeutic strategy for the eradication of bacterial pathogens without affecting the healthy mammalian cells.

[Retracted] Synthesis of Zinc Oxide (ZnO)‐Titanium Dioxide (TiO2)‐Chitosan‐Farnesol Nanocomposites and Assessment of Their Anticancer Potential in Human Leukemic MOLT‐4 Cell Line

“…The living (control) cells fluoresced green due to the diffusion of AO into the cellular membranes, but the treated cells turned apoptotic and fluoresced orange due to nuclear shrinkage. An earlier study conducted with ZnO/TiO 2 /Ag NCs also revealed similar observations [ 47 ]. The chromatin fragmentation that occurred in the NCs-treated cells indicated an uptake of EtBr dye, as observed with chitosan decorated silver nanoparticles in an earlier report [ 48 ].…”

Green synthesis of ZnO-TiO2/RGO nanocomposites using Senna surattensis extract: a novel approach for enhanced anticancer efficacy and biocompatibility

“…8a and b ), as shown in the previous study. 56 It can be seen in Fig. 8a and b that the cell viability was highly decreased for ZnO-TiO 2 NC due to the addition of the ZnO sheet due to their increasing surface charges.…”

Immobilization of ZnO-TiO2 Nanocomposite into Polyimidazolium Amphiphilic Chitosan Film, Targeting Improving Its Antimicrobial and Antibiofilm Applications

“…Finally, the presence of both hydrophilic and hydrophobic groups allows amphiphilic chitosan to self-assemble into micelles, which can enhance its solubility and bioavailability, as well as provide a means for the targeted delivery of the antimicrobial agent [52,53]. S2, Supplementary Materials) [61][62][63][64]. However, the newly synthesized PIACSB-coated TiO 2 -ZnO nanocomposite is particularly exciting due to its amazing antimicrobial activity (MIC/MBC→0.34/0.68 µg/mL, 0.20/0.40 µg/mL, and 0.15/0.30 µg/mL against S. aureus, E. coli, and P. aeruginosa, respectively) and unique properties.…”