Fabrication of vascular endothelial growth factor antibody bioconjugated ultrasmall near-infrared fluorescent Ag2S quantum dots for targeted cancer imaging in vivo

Abstract

BSA-stabilized Ag2S QDs were fabricated in aqueous solution via a one-pot approach, and subsequently bioconjugated with antiVEGF for targeted in vivo cancer imaging.

Near‐Infrared Fluorescent Ag2Se–Cetuximab Nanoprobes for Targeted Imaging and Therapy of Cancer

“…The optical properties, including absorption spectra and fluorescence emission spectra, of water‐soluble Ag 2 Se–OPA QDs and Ag 2 Se–PEG QDs should be one of the most concerns. As shown in Figure D, Ag 2 Se–OPA QDs and Ag 2 Se–PEG QDs still retained excellent NIR fluorescence emission, and the quantum yields were, respectively, up to 7.8% and 7.3%, which exceeded the lower limit (>1%) for NIR bioimaging . The fluorescence stability of water‐soluble Ag 2 Se QDs was also studied by monitoring the fluorescence intensity change of Ag 2 Se QDs during the storage in atmospheric environment at 4 °C for more than one month.…”

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

“…[17] Wang et al fabricated antibody-functionalized Ag 2 S QDs featuring high near infrared (NIR) fluorescence intensity, small size and low in vivo toxicity. [18] The Ag 2 S QDs were covalently immobilized with antibodies against vascular endothelial growth factor (VEGF). These conjugates were successfully demonstrated for targeted imaging of VEGF positive human glioblastoma tumors within mice.…”

“…[34–37] Improved oriented antibody adsorption has been reported using different pretreatment to either antibodies or to the supports, including UV light,[38] electric field, [39] electrochemistry,[40] and plasma-immersion ion implantation [18]. Jian and group has described that certain degree of orientation can be achieved for passively adsorbed IgG 1 and IgG 2a type antibodies on charged surfaces.…”

Near-infrared quantum dots based fluorescent assay of Cu2+ and in vitro cellular and in vivo imaging

“…didates for tissue imaging and biomarker detection. For instances, Yan's group has reported the use of Ag 2 S QDs for imaging the cancer cells [41]. CdTeSe/ZnS NIR QDs were utilized for the insulin detection in complex human plasma based on the NIR fluorescence energy transfer (FRET) [42].…”