Fabrication of Thermoresponsive Polymer Gradients for Study of Cell Adhesion and Detachment

Li, Linhui; Zhu, Yang; Li, Bo; Gao, Changyou

doi:10.1021/la802556e

Cited by 123 publications

(114 citation statements)

References 62 publications

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“…[60] This phenomenon was also observed for PNIPAAm brushes grafted on silicon and tissue-culture polystyrene (TCPS) surfaces. [61,62] Thermo-responsive cell adhesion/detachment behavior was found to weaken or even disappear when the PNIPAAm layer thickness was beyond a ''critical'' value.…”

Section: Stimuli-responsive Polymers and Diblock Copolymers For Surfamentioning

confidence: 99%

Surface Modification to Control Protein/Surface Interactions

Lin

et al. 2011

Macromolecular Bioscience

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Protein/surface interactions are well known to play an important role in various biological phenomena and to determine the ultimate biofunctionality of a given material once it is in contact with a biological environment. Control over the interactions between proteins and material surfaces are not only of great theoretical interest but also of crucial importance for many biomedical applications. In this Feature Article, we summarize various successful approaches used in our laboratory and other groups for controlling protein adsorption through chemical modification of the surface and/or the introduction of specific topographic features. Some perspectives on future research in these areas are also presented.

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Section: Stimuli-responsive Polymers and Diblock Copolymers For Surfamentioning

confidence: 99%

Surface Modification to Control Protein/Surface Interactions

Lin

et al. 2011

Macromolecular Bioscience

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“…As shown in Figure 5b, the cells did not exist on the surface of the silica-HBPS-g-PNIPAM at either 37 or 25 1C. According to the literature regarding PNIPAM-grafted-silicon substrates, 23 cell adhesion onto and detachment from PNIPAM-modified substrates did not work well when the thickness of the grafted PNIPAM was insufficient. The short length of grafted PNIPAM forms into a dense film structure on the surface of the substrate.…”

Section: Pnipam (mentioning

confidence: 86%

Poly(N-isopropylacrylamide)-modified silica beads with hyperbranched polysiloxysilane for three-dimensional cell cultivation

Park

Nabae

Surapati

et al. 2012

Polym J

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The immobilization of poly(N-isopropylacrylamide) (PNIPAM) onto the surface of silica beads via hyperbranched polysiloxysilane (HBPS) was studied to develop a new material for cell cultivation. HBPS terminated with a vinyl functional group was synthesized by self-polymerization of an AB 2 monomer. The terminal vinyl group was converted into a chain-transfer agent for reversible addition fragmentation chain transfer (RAFT) polymerization. The obtained HBPS was immobilized on the silica surface by mixing silica beads and HBPS in hexane. The graft polymerization of PNIPAM onto the silica surface was achieved using RAFT polymerization. The molecular weights of the grafted polymers obtained by cleavage from the surface of silica beads using NaOH aqueous solution were in the range of 6000-23 000 and the polydispersity index values were 1.4-1.6. The grafted silica beads have been tested for cell cultivation. Silica-HBPS-g-PNIPAM with a higher molecular weight (M n ) of PNIPAM (cleaved PNIPAM M n ¼ 14 800 and 23 700) showed a thermo-reversible phase transition at approximately 34 1C, and controlled cell adhesion and detachment was demonstrated with these beads.

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“…Other groups reported that plasma activation and plasma polymerization were also available for the surface modification with PIPPAm [41]. Recently, to precisely control chain length and density of grafted PIPAAm on the surfaces, atom transfer radical polymerization (ATRP) or reversible addition-fragmentation chain transfer (RAFT) polymerization are also used, which contributes to enhanced handling of cell attachment or detachment [42][43][44][45][46]. Furthermore, we have also developed the method to fabricate a cell sheet using the thermoresponsive surfaces that are prepared via a spin-coating method with a block copolymer consisting of PIPAAm and poly(butyl methacrylate) (PBMA) on polystyrene surfaces [47].…”

Section: Cell Sheet Engineering Based On Temperatureresponsive Culturmentioning

confidence: 99%

Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

et al. 2013

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Recently, regenerative medicine has been focused on as next-generation definitive therapies for several diseases or injuries for which there are currently no effective treatments. These therapies have been rapidly developed through the effective fusion between different fields such as stem cell biology and biomaterials. So far, we have proposed "cell sheet engineering" through our core technology that simply applies alterations of the temperature which allows regulation of the attachment or detachment of living cells on the culture surfaces grafted with the temperature-responsive polymer "poly(N-isoproplyacrylamide)". This technology enables us to construct bioengineered sheet-like tissues, termed "cell sheets", without the need of using biodegradable scaffolds and protease treatments that are traditionally used. Therefore, our carrier-free cell sheets not only are independent of the issues observed in conventional methods, but also showed further advantages in the reconstruction of the corneal epithelium or endothelium (e.g. improvement of optical transparency and cell-cell interactions to host stroma in reconstructed tissues). Moreover, our approach does not have issues such as immune rejection or limited number of donors, due to the use of cell sheets derived from autologous limbal (in patients with unilateral disease) or oral mucosal epithelial cells (in patients with bilateral disorders). Indeed, we have successfully performed the clinical application for the reconstruction of ocular surfaces through the transplantation of our carrier-free corneal epithelial cell sheets, as evidenced by improved visual acuity as well as long-term maintenance of postoperative health conditions on ocular surfaces in all patients. We have also proposed a novel approach for the reconstruction of the corneal endothelium using corneal endothelial cell sheets by demonstrating its successful application. Thus, our cell sheet engineering technique provides a breakthrough in the field of regenerative medicine applied to the cornea. cell sheet, temperature-responsive, carrier-free Citation:Umemoto T, Yamato M, Nishida K, et al. Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces. Chin Sci Bull, 2013, 58: 43494356,

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Fabrication of Thermoresponsive Polymer Gradients for Study of Cell Adhesion and Detachment

Cited by 123 publications

References 62 publications

Surface Modification to Control Protein/Surface Interactions

Surface Modification to Control Protein/Surface Interactions

Poly(N-isopropylacrylamide)-modified silica beads with hyperbranched polysiloxysilane for three-dimensional cell cultivation

Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

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