Fabrication of magnetic ordered mesoporous carbon for quantitative analysis of acidic phytohormones in mushroom samples prior to their determination by ultra-high-performance liquid chromatography–tandem mass spectrometry

Zhang, Qianchun; Chen, Yan; Yang, Yanqun; Liu, Yulan; Wen, Ming; Wang, Xingyi

doi:10.1556/1326.2022.01022

Cited by 2 publications

(1 citation statement)

References 35 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typically, ACN extraction is used in non-targeted metabolomic studies ( Yu et al., 2023a ). Chemical derivatization is essential for gas chromatography but not LC-MS-based multitargeted profiling since most known phytohormones are non-volatile substances ( Nwogha et al., 2023 ; Zhang et al., 2023 ). The versatility of LC methods has been significantly enhanced with the growing availability of chromatographic columns with various physicochemical features.…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Wang,

Sarsaiya,

Qian

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Pinellia ternata (Thunb.) Breit. (Araceae), a significant medicinal plant, has been used to treat various diseases for centuries. Terahertz radiation (THZ) is located between microwaves and infrared rays on the electromagnetic spectrum. THZ possesses low single-photon energy and a spectral fingerprint, but its effects on plant growth have not yet been investigated. The study’s primary objective was to examine the transcriptome and metabolome databases of the SY line to provide a new perspective for identifying genes associated with resistance and growth promotion and comprehending the underlying molecular mechanism. Variations in the biological characteristics of P. ternata grown under control and experimental conditions were analyzed to determine the effect of THZ. Compared with the control group, phenotypic variables such as leaf length, petiole length, number of leaves, leaf petiole diameter, and proliferation coefficient exhibited significant differences. P. ternata response to THZ was analyzed regarding the effects of various coercions on root exudation. The experimental group contained considerably more sugar alcohol than the control group. The transcriptome analysis revealed 1,695 differentially expressed genes (DEGs), including 509 upregulated and 1,186 downregulated genes. In the KEGG-enriched plant hormone signaling pathway, there were 19 differentially expressed genes, 13 of which were downregulated and six of which were upregulated. In the metabolomic analysis, approximately 416 metabolites were uncovered. There were 112 DEMs that were downregulated, whereas 148 were upregulated. The P. ternata leaves displayed significant differences in phytohormone metabolites, specifically in brassinolide (BR) and abscisic acid (ABA). The rise in BR triggers alterations in internal plant hormones, resulting in faster growth and development of P. ternata. Our findings demonstrated a link between THZ and several metabolic pathway processes, which will enhance our understanding of P. ternata mechanisms.

show abstract

Section: Discussion and Perspectivesmentioning

confidence: 99%

Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Wang,

Sarsaiya,

Qian

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Spatio-temporal plant hormonomics: from tissue to subcellular resolution

Petřík,

Hladík,

Zhang

et al. 2024

Journal of Experimental Botany

View full text Add to dashboard Cite

Due to technological advances in mass spectrometry, significant progress has been achieved recently in plant hormone research. Nowadays, plant hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly on their isolation, identification and spatiotemporal quantification in plants. This review represents a comprehensive meta-study of the advances in the phytohormone analysis by mass spectrometry over the past decade. To address current trends and future perspectives, Web of Science data were systematically collected and key features such as mass spectrometry-based analyses were evaluated using multivariate data analysis methods. Our findings showed that plant hormonomics is currently divided into targeted and untargeted approaches. Both aim to miniaturize the sample, allowing high-resolution quantification to be covered in plant organs as well as subcellular compartments. Therefore, we can study plant hormone biosynthesis, metabolism and signalling at a spatio-temporal resolution. Moreover, this trend has recently been accelerated by technological advances such as fluorescence-activated cell sorting or mass spectrometry imaging.

show abstract

Fabrication of magnetic ordered mesoporous carbon for quantitative analysis of acidic phytohormones in mushroom samples prior to their determination by ultra-high-performance liquid chromatography–tandem mass spectrometry

Cited by 2 publications

References 35 publications

Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Spatio-temporal plant hormonomics: from tissue to subcellular resolution

Contact Info

Product

Resources

About